A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}l(1)G0007EP764 Insertion

General Information
Symbol Dmel\P{EP}l(1)G0007EP764 Species D. melanogaster
Name FlyBase ID FBti0007326
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) l(1)G0007 Viability / fertility viable
Causes allele(s) l(1)G0007EP764 Stock availability 1 publicly available
LINE ID EP(X)0764
Genomic Location
Chromosomal location X ( 12E5 ) Sequence location X:14,116,361..14,116,361 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0038214 FBti0039397 FBti0012847 FBti0069961 FBti0048941 FBti0068262 FBti0067181 FBti0038770 FBti0045949 FBti0053758 FBti0056347 FBti0107693 FBti0036760 FBti0048903 FBti0147204 FBti0071291 FBti0071741 FBti0013211 FBti0015513 FBti0107576 FBti0106962 FBti0015631 FBti0028541 FBti0104308 FBti0007332 FBti0028552 FBti0020905 FBti0059352 FBti0111881 FBti0015668 FBti0007326 FBti0013891 FBti0071959 FBti0041787
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
X:14,116,361..14,116,361 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
12E5 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
12E1-12E1 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
12E4-12E5 (in situ hybridization reported)
(Spradling, 1997.8.22)
12E4-12E5 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ254753
(FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
l(1)G0007
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
l(1)G0007EP764
(Kraut et al., 2001, Gene Disruption Project members, 2001-)
Lethality
References
viable
(Spradling, 1997.8.22)
Sterility
References
hide Phenotype Manifest In
anterior fascicle & synapse
(Kraut et al., 2001)
intersegmental nerve
(Kraut et al., 2001)
hide Detailed Description
Statement
Reference
Mutant larvae expressing l(1)G0007EP764 under the control of Scer\GAL4elav-C155 have ISN neuron pathfinding defects. Mutant larvae expressing l(1)G0007EP764 under the control of Scer\GAL4elav-C155 have reduced or abnormal synapses.
(Kraut et al., 2001)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(X)0764
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
10123
w1118 P{EP}l(1)G0007EP764
hide Linkouts
hide Comments
Location X:14056265-14056266 confirmed by FlyBase alignment of dbGSS accession AQ254753 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(x) 0764
(Kraut et al., 2001)
EP(X)764
(McGovern et al., 2003)
EP(X)0764
(FlyBase, 1992-)
P{EP}CG1405EP764
(FlyBase, 1992-)
P{EP}CG32604EP764
(FlyBase, 1992-)
P{EP}EP764
(FlyBase, 1992-)
P{EP}EP764EP764
(FlyBase, 1992-)
P{EP}l(1)G0007EP764
(FlyBase, 2005, FlyBase, 1992-, )
P{w}EP764
(Spradling, 1997.8.22)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
McGovern et al., 2003, Mech. Dev. 120(10): 1193--1207
A targeted gain of function screen in the embryonic CNS of Drosophila. [FBrf0167840]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material
Kraut et al., 2001, Curr. Biol. 11(6):
A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
Spradling, 1997.8.22, EP lines from BDGP.
EP lines from BDGP. [FBrf0100829]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]