Dmel\P{EP}futsch^EP1419 Insertion

General Information
Symbol	Dmel\P{EP}futsch^EP1419	Species	D. melanogaster
Name		FlyBase ID	FBti0007440
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	CG11448, futsch	Viability / fertility
Causes allele(s)	futsch^EP1419	Stock availability	1 publicly available
LINE ID	EP(X)1419
Genomic Location
Chromosomal location	X ( 2A1 )	Sequence location	X:1,293,932..1,293,932 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	1 (BDGP Project Members, 1994-1999)
Sequence Location	X:1,293,932..1,293,932 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	2A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	1E1-1E1 (reported as inferred from sequence location) (BDGP Project Members, 2000-) 2A1-2A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025455 (Benos et al., 2001, FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	CG11448 (BDGP Project Members, 2000-) futsch (Hummel et al., 2000, Zhang et al., 2001, Roos et al., 2000, Gene Disruption Project members, 2001-, Benos et al., 2001, Lee et al., 2010, Gogel et al., 2006)
Alleles and Phenotypes
Causes alleles	futsch^EP1419 (Hummel et al., 2000, Zhang et al., 2001, Roos et al., 2000, Gene Disruption Project members, 2001-, Benos et al., 2001, Lee et al., 2010, Gogel et al., 2006)

Lethality References
Sterility References
Phenotype Manifest In
	bouton (Roos et al., 2000, Zhang et al., 2001) photoreceptor cell (Zhang et al., 2001)
Detailed Description
Statement Reference Co-expression of futsch[EP1419] pre-synaptically rescues the synaptic overgrowth phenotype found upon expression of Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] pre-synaptically under the control of Scer\GAL4[elav-C155]. However, synaptic defects caused by post-synaptic expression of Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] are not suppressed when futsch[EP1419] is expressed post-synaptically (under the control of Scer\GAL4[Mhc.PW]). The abnormal microtubule phenotype seen upon Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] presynaptic expression is rescued by a futsch[EP1419] expression (both under the control of Scer\GAL4[elav-C155]). Neuronal co-expression of futsch[EP1419] with Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] rescues the mitochondrial distribution phenotype seen upon Hsap\LRRK2[Scer\UAS.T:Zzzz\FLAG] expression alone. (Lee et al., 2010) Expression of futsch[EP1419] pre-synaptically, under the control of Scer\GAL4[elav-C155] has no significant effect on synaptic mitochondria. (Lee et al., 2010) When expression is driven by Scer\GAL4^elav-C155 the phenotype is similar to that of Fmr1 loss of function mutants - neuromuscular junction overgrowth with increased synaptic area, branching and bouton number. Neurotransmission at the NMJ is elevated, and in the photoreceptor is depressed. (Zhang et al., 2001) Expression of futsch^EP1419 and Rnor\MAP2^Scer\UAS.cAa driven by Scer\GAL4⁴⁴ results in frequent axonal swellings and misprojection phenotypes. In 1/5 of neuromeres sensory neurons project their axons in on one of the two commissures across the central nervous system (CNS) midline and fasciculate with their contralateral counterparts, which they never do in wild-type. (Hummel et al., 2000) When expression of futsch^EP1419 is driven by Scer\GAL4^sim.PS, changes in the morphology of individual growth cones is seen, and minor defects in the projection of individual axons are also seen. However these early axonal phenotypes do not lead to defects in the later formation of the central nervous system (CNS) axon pattern. (Hummel et al., 2000) When futsch^EP1419 is expressed under the control of Scer\GAL4^elav-C155, a slight reduction in average bouton size is seen (about 7.1μm², compared to 9.1μm² in wild-type). (Roos et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(X)1419 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10751 P{EP}futsch^EP1419 w¹¹¹⁸
Linkouts
Comments
	Location X:1256720-1256721 confirmed by FlyBase alignment of dbGSS accession AQ025455 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) Maps to clone: 132E8 (Benos et al., 2001) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP1419 (Lee et al., 2010, Gogel et al., 2006) EP(X)1419 (Hummel et al., 2000, FlyBase, 1992-) ep(X)1419 (Roos et al., 2000) ep(x)1419 (FlyBase, 1992-) P{EP}EP1419 (FlyBase, 1992-) P{EP}futsch^EP1419 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 14 )
Research paper	Lee et al., 2010, J. Neurosci. 30(50): 16959--16969 LRRK2 kinase regulates synaptic morphology through distinct substrates at the presynaptic and postsynaptic compartments of the Drosophila neuromuscular junction. [FBrf0212570] Gogel et al., 2006, Mol. Cell. Neurosci. 33(2): 188--199 The Drosophila microtubule associated protein Futsch is phosphorylated by Shaggy/Zeste-white 3 at an homologous GSK3beta phosphorylation site in MAP1B. [FBrf0194917] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Benos et al., 2001, Genome Res. 11(5): 710--730 From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823] Zhang et al., 2001, Cell 107(5): 591--603 Drosophila fragile X-related gene regulates the MAP1B homolog Futsch to control synaptic structure and function. [FBrf0141416] Hummel et al., 2000, Neuron 26(2): 357--370 Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development. [FBrf0128497] Roos et al., 2000, Neuron 26(2): 371--382 Drosophila Futsch regulates synaptic microtubule organization and is necessary for synaptic growth. [FBrf0128626] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}futschEP1419 Insertion

Dmel\P{EP}futsch^EP1419 Insertion