A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{EP}pAbpEP310 Insertion

General Information
Symbol Dmel\P{EP}pAbpEP310 Species D. melanogaster
Name FlyBase ID FBti0007560
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) pAbp Viability / fertility
Causes allele(s) pAbpEP310 Stock availability 1 publicly available
LINE ID EP(2)0310
Genomic Location
Chromosomal location 2R ( 55B9 ) Sequence location 2R:14,033,247..14,033,247 [-]
Map ( GBrowse ) detailed view FBti0026235 FBti0115174 FBti0069648 FBti0125264_2 FBti0125264_1 FBti0103360 FBti0030301 FBti0057651 FBti0111634 FBti0107525 FBti0107912 FBti0006528 FBti0025783 FBti0143710 FBti0007560 FBti0141108 FBti0076360 FBti0141141 FBti0006611 FBti0125265_2 FBti0125265_1 FBti0125266_2 FBti0124405 FBti0053661 FBti0040287 FBti0051994 FBti0046495 FBti0125266_1 FBti0039424 FBti0105372
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
2
(BDGP Project Members, 1994-1999)
Sequence Location
2R:14,033,247..14,033,247 [-]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
55B9 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
55B5-55B6 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
55B7-55B7 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025970
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
pAbp
(BDGP Project Members, 2000-, Blagden et al., 2009, Cziko et al., 2009, Pertceva et al., 2010)
hide Alleles and Phenotypes
Causes alleles
pAbpEP310
(Sigrist et al., 2000, Reiff et al., 2002, Gene Disruption Project members, 2001-, Hekmat-Scafe et al., 2005, Blagden et al., 2009, Cziko et al., 2009, Pertceva et al., 2010)
Lethality
References
viable
(Pertceva et al., 2010)
Sterility
References
male sterile
(Blagden et al., 2009)
hide Phenotype Manifest In
bouton
(Sigrist et al., 2000)
class IV dendritic arborizing neuron | somatic clone
(Cziko et al., 2009)
spindle & spermatocyte
(Blagden et al., 2009)
hide Detailed Description
Statement
Reference
17% of homozygous and 18% of pAbp[EP310]/pAbp[k10109] spermatocytes show defects in meiosis, with the most common phenotype being detachment of the meiotic spindles from astral microtubules.
(Blagden et al., 2009)
23% of pAbp[EP310]/+ ; larp[mtr-null]/+ double heterozygous spermatocytes show defects in meiosis. 16% of pAbp[EP310]/+ ; larp[S003108]/+ double heterozygous spermatocytes show defects in meiosis. The frequency of homozygous pAbp[EP310] spermatocytes that show defects in meiosis (17%) is increased by larp[mtr-null]/+ (to 31.4%) or larp[S003108]/+ (to 29.3%).
(Blagden et al., 2009)
Over-expression of pAbp[EP310] reduces dendrite branching, and alters dendritic morphology in larval class IV sensory neurons (induced under the control of Scer\GAL4[Act.PU]). Total dendrite length is significantly reduced in these neurons. The rough eye phenotype observed in Fmr1[sev.PW] flies is suppressed in a pAbp[EP310]/+ background.
(Cziko et al., 2009)
The density of T-bars in the boutons of pAbpEP310/Df(2R)Pcl7B mutants is the same as wild type.
(Reiff et al., 2002)
The mean amplitude of miniature postsynaptic currents (mEJCs) at the neuromuscular junction is indistinguishable from wild type in pAbpEP310/Df(2R)Pcl7B flies. Evoked postsynaptic currents (eEJCs) are significantly larger than in wild-type. The number of junctional boutons per neuromuscular junction is increased compared to wild type.
(Sigrist et al., 2000)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)0310
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
17261
w1118; P{EP}pAbpEP310
hide Linkouts
hide Comments
Location 2R:13660605-13660606 confirmed by FlyBase alignment of dbGSS accession AQ025970 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(2)310
(Hekmat-Scafe et al., 2005)
ep(2)0310
(FlyBase, 1992-)
EP(2)0310
(FlyBase, 1992-)
P{EP}EP310
(FlyBase, 1992-)
P{EP}pAbpEP310
(FlyBase, 2005, FlyBase, 1992-, Blagden et al., 2009, Pertceva et al., 2010)
P{EP}pAbpEP0310
(FlyBase, 1992-)
pabpEP310
(Cziko et al., 2009)
Secondary FlyBase IDs
hide References ( 14 )
Research paper
Pertceva et al., 2010, Cell Biol. Int. 34(10): 991--996
The role of Drosophila hyperplastic discs gene in spermatogenesis. [FBrf0211767]
Blagden et al., 2009, Dev. Biol. 334(1): 186--197
Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development. [FBrf0208825]
Cziko et al., 2009, Genetics 182(4): 1051--1060
Genetic Modifiers of dFMR1 Encode RNA Granule Components in Drosophila. [FBrf0208636]
Hekmat-Scafe et al., 2005, Genetics 169(3): 1477--1493
Seizure suppression by gain-of-function escargot mutations. [FBrf0183998]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Reiff et al., 2002, J. Neurosci. 22(21): 9399--9409
Differential regulation of active zone density during long-term strengthening of Drosophila neuromuscular junctions. [FBrf0152148]
Sigrist et al., 2000, Nature 405(6790): 1062--1065
Postsynaptic translation affects the efficacy and morphology of neuromuscular junctions. [FBrf0128816]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0125078]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]