Dmel\P{EP}HSPC300^EP506 Insertion

General Information
Symbol	Dmel\P{EP}HSPC300^EP506	Species	D. melanogaster
Name		FlyBase ID	FBti0007613
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	CG3163, HSPC300	Viability / fertility	viable
Causes allele(s)	HSPC300^EP506, HSPC300^Δ54.3	Stock availability	1 publicly available
LINE ID	EP(2)0506
Genomic Location
Chromosomal location	2R ( 60B4 )	Sequence location	2R:19,916,330..19,916,330 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	2 (BDGP Project Members, 1994-1999)
Sequence Location	2R:19,916,330..19,916,330 [-] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	60B4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	60B1-60B1 (reported as inferred from sequence location) (BDGP Project Members, 2000-) 60B1-60B4 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026024 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	CG3163 (BDGP Project Members, 2000-) HSPC300 (Zhu and Bhat, 2011, Qurashi et al., 2007)
Alleles and Phenotypes
Causes alleles	HSPC300^EP506 (Zhu and Bhat, 2011, Qurashi et al., 2007) HSPC300^Δ54.3 (Qurashi et al., 2007)

Lethality References lethal \| embryonic stage \| maternal effect \| recessive (Qurashi et al., 2007) lethal \| partially (Qurashi et al., 2007) lethal \| pupal stage \| recessive (Qurashi et al., 2007) viable (Qurashi et al., 2007)
Sterility References
Phenotype Manifest In
	embryonic/larval neuromuscular junction (Qurashi et al., 2007) longitudinal connective \| maternal effect (Qurashi et al., 2007) mesothoracic bristle (Qurashi et al., 2007) presumptive embryonic/larval central nervous system \| maternal effect (Qurashi et al., 2007) ventral nerve cord commissure \| maternal effect (Qurashi et al., 2007)
Detailed Description
Statement Reference Homozygotes are 100% viable. HSPC300[EP506]/HSPC300[Δ54.3] animals show 30% viability. Zygotic null, maternal hypomorph embryos (derived from HSPC300[EP506]/HSPC300[Δ54.3] females mated to HSPC300[Δ54.3]/+ males) sometimes show abnormal crossing of the midline by axons (10% of embryos), and in the most severe cases, axons ectopically cross the midline several times. Commissures and longitudinal connectives are not properly formed. (Qurashi et al., 2007) Homozygotes die at the late pupal stage. Homozygous embryos (lacking zygotic HSPC300 function) have normal central nervous system axon morphology. Homozygous embryos derived from homozygous female germline clones (lacking zygotic and maternal HSPC300 function) show severe, though variable, nervous system defects ranging from broken and disorganised longitudinal connectives and commissures to remnants of axons or depletion of all central nervous system axon bundles. In addition, lethality is shifted to embryonic stages in these animals and the embryos appear overall disorganised. HSPC300[EP506]/HSPC300[Δ54.3] animals show 30% viability. Zygotic null, maternal hypomorph embryos (derived from HSPC300[EP506]/HSPC300[Δ54.3] females mated to HSPC300[Δ54.3]/+ males) sometimes show abnormal crossing of the midline by axons (10% of embryos), and in the most severe cases, axons ectopically cross the midline several times. Commissures and longitudinal connectives are not properly formed. The neuromuscular junctions of homozygous larvae are severely reduced in length (to 66% of wild type) and have supernumerary buds (88% increase compared to wild type). Heterozygous larvae also show a significant reduction in synaptic length compared to wild type. Homozygous pharate adults occasionally have bent thoracic bristles, although the number of bristles on the head and thorax are normal. (Qurashi et al., 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)0506 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17173 w¹¹¹⁸; P{EP}HSPC300^EP506
Linkouts
Comments
	Location 2R:19536407-19536408 confirmed by FlyBase alignment of dbGSS accession AQ026024 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	ep(2)0506 (FlyBase, 1992-) EP(2)0506 (FlyBase, 1992-) EP(2R)0506 (Qurashi et al., 2007) P{EP}EP506 (FlyBase, 2005, FlyBase, 1992-) P{EP}HSPC300^EP506 (FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 10 )
Research paper	Zhu and Bhat, 2011, Dev. Biol. 357(2): 283--294 The Hem protein mediates neuronal migration by inhibiting WAVE degradation and functions opposite of Abelson tyrosine kinase. [FBrf0214765] Qurashi et al., 2007, Neural Dev. 2: 18 HSPC300 and its role in neuronal connectivity. [FBrf0205239] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}HSPC300EP506 Insertion

Dmel\P{EP}HSPC300^EP506 Insertion