A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}zipEP856 Insertion

General Information
Symbol Dmel\P{EP}zipEP856 Species D. melanogaster
Name FlyBase ID FBti0007719
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) zip Viability / fertility
Causes allele(s) zipEP856 Stock availability none publicly available
LINE ID EP(2)0856
Genomic Location
Chromosomal location 2R ( 60E12 ) Sequence location 2R:20,898,638..20,898,638 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0143534 FBti0143064 FBti0143667 FBti0053564 FBti0099952 FBti0005226 FBti0102340 FBti0108492 FBti0110963 FBti0026313 FBti0007719 FBti0055898 FBti0125437_2 FBti0125437_1 FBti0067115 FBti0007775 FBti0104033 FBti0110106 FBti0036965 FBti0125438_1 FBti0125438_2 FBti0067691 FBti0046700 FBti0067694 FBti0051114 FBti0043207 FBti0066644 FBti0036247 FBti0125439 FBti0147303 FBti0050030
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2
(BDGP Project Members, 1994-1999)
Sequence Location
2R:20,898,638..20,898,638 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
60E12 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
60E8-60E8 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
60F1-60F3 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ024980
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
zip
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
zipEP856
(Gene Disruption Project members, 2001-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)0856
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 2R:20518715-20518716 confirmed by FlyBase alignment of dbGSS accession AQ024980 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
ep(2)0856
(FlyBase, 1992-)
EP(2)0856
(FlyBase, 1992-)
P{EP}EP856
(FlyBase, 1992-)
P{EP}zipEP856
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]