A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}Reg-5EP1063 Insertion

General Information
Symbol Dmel\P{EP}Reg-5EP1063 Species D. melanogaster
Name FlyBase ID FBti0007762
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) Reg-5 Viability / fertility
Causes allele(s) Reg-5EP1063 Stock availability 1 publicly available
LINE ID EP(2)1063
Genomic Location
Chromosomal location 2R ( 60E1 ) Sequence location 2R:20,680,883..20,680,883 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0115687 FBti0115609 FBti0042423 FBti0041064 FBti0007763 FBti0007762 FBti0055248 FBti0054949 FBti0053027 FBti0070014 FBti0052478 FBti0149019
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
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What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
2
(BDGP Project Members, 1994-1999)
Sequence Location
2R:20,680,883..20,680,883 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
60E1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
60D16-60D16 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ025022
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Reg-5
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
Reg-5EP1063
(Gene Disruption Project members, 2001-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)1063
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
17212
w1118; P{EP}Reg-5EP1063
hide Linkouts
hide Comments
Location 2R:20300960-20300961 confirmed by FlyBase alignment of dbGSS accession AQ025022 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
ep(2)1063
(FlyBase, 1992-)
EP(2)1063
(FlyBase, 1992-)
P{EP}EP1063
(FlyBase, 1992-)
P{EP}Reg-5EP1063
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]