Dmel\P{EP}Nmdar1^EP331 Insertion

General Information
Symbol	Dmel\P{EP}Nmdar1^EP331	Species	D. melanogaster
Name		FlyBase ID	FBti0007801
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Nmdar1	Viability / fertility
Causes allele(s)	Nmdar1^EP331	Stock availability	1 publicly available
LINE ID	EP(3)0331
Genomic Location
Chromosomal location	3R ( 83A6 )	Sequence location	3R:1,334,116..1,334,116 [+]
Map ( GBrowse )
Recent Updates
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)	3 (BDGP Project Members, 1994-1999)
Sequence Location	3R:1,334,116..1,334,116 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	83A6 ( inferred by FlyBase from sequence location )
Cytological location (reported)	83A7-83A7 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ025064 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Nmdar1 (BDGP Project Members, 2000-, Zhou et al., 2010, Qin and Dubnau, 2010)
Alleles and Phenotypes
Causes alleles	Nmdar1^EP331 (Xia et al., 2005, Zhou et al., 2010, Qin and Dubnau, 2010)

Lethality References viable \| recessive (Xia et al., 2005)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Nmdar1[EP331] mutants exhibit reduced short-term and long-term memory. Extinction learning appears to be normal in these mutants. (Qin and Dubnau, 2010) rut[1]/+; Nmdar1[EP331]/Nmdar1[EP331] double mutants exhibit significantly lower performance in standard Pavlovian learning assays compared to either single mutant. (Qin and Dubnau, 2010) Disruption of Nmdar1 through expression of Nmdar1[EP331] under the control of Scer\GAL4[hs.PB] does not result in a significant decrease in PI (preference index - with respect to light) in constant darkness conditions compared with control flies (indicating that phototaxis preference behaviour plasticity is inhibited by down-regulation of Nmdar1 during the critical period). Nmdar1[EP331]/Scer\GAL4[hs.PB] flies reared in a normal light/dark cycle for 4 days at 18[o]C that are then treated with heat-shock and transferred to constant darkness exhibit no significant difference in PI (preference index - with respect to light) in constant darkness conditions compared with control flies. (Zhou et al., 2010) Olfactory learning after Pavlovian conditioning is reduced in Nmdar1^EP331 mutants compared to wild-type flies. However, sensorimotor responses to odors and foot shock stimuli are not affected. Expression of one copy of Nmdar1^EP331 under the control of one copy of Scer\GAL4^P26.hs, following heat shock, yields an antisense transcript of Nmdar1. This acute repression of Nmdar1 leads to a transient, severe disruption of olfactory learning. This acute repression also disrupts one day training after spaced training, while one day memory after massed training is normal, showing that long term memory is defective, but anesthesia-resistant memory is normal. Sensorimotor responses to odors and foot shock stimuli are not affected. (Xia et al., 2005)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)0331 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17112 w¹¹¹⁸; P{EP}Nmdar1^EP331
Linkouts
Comments
	insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	dNR1(EP331) (Qin and Dubnau, 2010) dsNR1 (Zhou et al., 2010) EP331 (Zhou et al., 2010) ep(3)0331 (FlyBase, 1992-) EP(3)0331 (FlyBase, 1992-) P{EP}EP331 (FlyBase, 1992-) P{EP}Nmdar1^EP331 (FlyBase, 1992-, Zhou et al., 2010)
Secondary FlyBase IDs

References ( 10 )
Research paper	Qin and Dubnau, 2010, Genes Brain Behav. 9(2): 203--212 Genetic disruptions of Drosophila Pavlovian learning leave extinction learning intact. [FBrf0210733] Zhou et al., 2010, Genes Brain Behav. 9(3): 325--334 NMDA receptors-dependent plasticity in the phototaxis preference behavior induced by visual deprivation in young and adult flies. [FBrf0210919] Xia et al., 2005, Curr. Biol. 15(7): 603--615 NMDA receptors mediate olfactory learning and memory in Drosophila. [FBrf0187341] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{EP}Nmdar1EP331 Insertion

Dmel\P{EP}Nmdar1^EP331 Insertion