Dmel\P{EP}faf^EP381 Insertion

General Information
Symbol	Dmel\P{EP}faf^EP381	Species	D. melanogaster
Name		FlyBase ID	FBti0007809
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	faf	Viability / fertility
Causes allele(s)	faf^EP381	Stock availability	1 publicly available
LINE ID	EP(3)0381
Genomic Location
Chromosomal location	3R ( 100D2 )	Sequence location	3R:27,603,009..27,603,009 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	3 (BDGP Project Members, 1994-1999)
Sequence Location	3R:27,603,009..27,603,009 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	100D2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	100E1-100E1 (reported as inferred from sequence location) (BDGP Project Members, 2000-) 100E1-100E2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025072 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	faf (BDGP Project Members, 2000-, Reiter et al., 2006)
Alleles and Phenotypes
Causes alleles	faf^EP381 (Abdelilah-Seyfried et al., 2000, Pena-Rangel et al., 2002, Watts et al., 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, DiAntonio et al., 2001, Collins et al., 2006, Reiter et al., 2006)

Lethality References viable (Stinchfield et al., 2012)
Sterility References
Phenotype Manifest In
	adult abdominal sensillum & trichogen cell (Abdelilah-Seyfried et al., 2000) anterior fascicle & bouton (Kraut et al., 2001) anterior fascicle & synapse (Kraut et al., 2001) anterior fascicle & synapse \| supernumerary (Kraut et al., 2001) bouton (Collins et al., 2006) bouton \| supernumerary (DiAntonio et al., 2001) dorsocentral bristle (Abdelilah-Seyfried et al., 2000) eye (Reiter et al., 2006) intersegmental nerve (Kraut et al., 2001) mushroom body (Watts et al., 2003) ommatidium (Reiter et al., 2006) scutellar bristle (Abdelilah-Seyfried et al., 2000) trichogen cell (Abdelilah-Seyfried et al., 2000) wing sensillum \| ectopic (Pena-Rangel et al., 2002)
Detailed Description
Statement Reference Animals derived from homozygous faf[EP381] females mated to dpp[hr4]/+ males show strong lethality (only 26% of the expected number survive). The embryos have a ventralised cuticle phenotype. (Stinchfield et al., 2012) Neural expression of faf^EP381, under the control of Scer\GAL4^elav-C155 leads to an increased number of synaptic boutons and branches and an increased synaptic span. (Collins et al., 2006) wnd¹/wnd² mutants suppress the synapse phenotype of faf^EP381, under the control of Scer\GAL4^elav-C155. (Collins et al., 2006) Expression of faf[EP381] under the control of Scer\GAL4[GMR.PF] at room temperature results in smooth glassy eyes (due to fusion of ommatidia) that are also moderately reduced in size. (Reiter et al., 2006) The faf[EP381] (Scer\GAL4[GMR.PF]) overexpression phenotype is strongly enhanced by co-expression with pbl[EP3415] as the ommatidial field is reduced to approximately 30% of normal in Scer\GAL4[GMR.PF]-driven pbl[EP3415] and faf[EP381] flies. (Reiter et al., 2006) The synergistic effect of pbl[EP3415] and faf[EP381] can be overcome by co-expression with Hsap\UBE3A[Scer\UAS.cRa] (all under the control of Scer\GAL4[GMR.PF]. Co-expression of Hsap\UBE3A[Scer\UAS.cRa] with faf[EP381] (both under the control of Scer\GAL4[GMR.PF]) partially suppresses the smooth eye phenotype induced by expression of faf[EP381] alone. (Reiter et al., 2006) Expression of faf^EP381 under the control of Scer\GAL4^Tab2-201Y results in misguidance and mushroom body overgrowth phenotypes. (Watts et al., 2003) pnr^MD237/+ adults expressing faf^EP381 under the control of Scer\GAL4^pnr-MD237 have a slight cleft in the abdominal dorsal midline and a reduced scutellum. (Pena-Rangel et al., 2002) Flies expressing faf^EP381 under the control of Scer\GAL4^ap-md544 have ectopic bristles on the dorsal surface of the wing blade. (Pena-Rangel et al., 2002) When faf^EP381 is driven by Scer\GAL4^elav-C155, a marked expansion in the size and complexity is seen in the synapses. There is an increase in both the number of synaptic boutons and in the synaptic span. There is also an increase in the number of synaptic branches. When the evoked excitatory junctional potentials (EJPs) of faf^EP381, Scer\GAL4^elav-C155 embryos are examined, a marked reduction is seen in their amplitudes. A large decrease in quantal content is also seen, and the frequency of spontaneous mEJPs is also reduced. (DiAntonio et al., 2001) In the dorsal muscle region of mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155, the synapses at the second branchpoint and terminal arbor have abnormal structures, with a spray of extra branches emanating from the terminal arbor. (Kraut et al., 2001) Mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. Mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155 have excessive or ectopic synapses. Mutant larvae expressing faf^EP381 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. They have abnormal ISN synaptic branches and a reduced number of boutons. (Kraut et al., 2001) Expression of faf^EP381 under the control of Scer\GAL4^sca-537.4 results in loss of external support cells of the scutellar and dorsocentral macrochaetae without loss of the sheath cell. Shafts with a branched tip are seen. On the abdomen, dot-like shaft morphology is seen. (Abdelilah-Seyfried et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)0381 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	6414 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{EP}faf^EP381
Linkouts
Comments
	Location 3R:27603009-27603010 confirmed by FlyBase alignment of dbGSS accession AQ025072 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3)381 (DiAntonio et al., 2001) EP(3) 0381 (Kraut et al., 2001, Kraut et al., 2001) ep(3)0381 (FlyBase, 1992-) EP(3)0381 (Collins et al., 2006) faf^EP(3)381 (Reiter et al., 2006) P{EP}EP381 (FlyBase, 1992-) P{EP}faf^EP381 (FlyBase, 2005, FlyBase, 1992-, ) P{EP}faf^EP0381 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 17 )
Research paper	Stinchfield et al., 2012, Development 139(15): 2721--2729 Fat facets deubiquitylation of Medea/Smad4 modulates interpretation of a Dpp morphogen gradient. [FBrf0219593] Collins et al., 2006, Neuron 51(1): 57--69 Highwire restrains synaptic growth by attenuating a MAP kinase signal. [FBrf0194919] Reiter et al., 2006, Hum. Mol. Genet. 15(18): 2825--2835 Expression of the Rho-GEF Pbl/ECT2 is regulated by the UBE3A E3 ubiquitin ligase. [FBrf0192865] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Watts et al., 2003, Neuron 38(6): 871--885 Axon pruning during Drosophila metamorphosis: evidence for local degeneration and requirement of the ubiquitin-proteasome system. [FBrf0161044] Pena-Rangel et al., 2002, Genetics 160(3): 1035--1050 A misexpression study examining dorsal thorax formation in Drosophila melanogaster. [FBrf0147055] DiAntonio et al., 2001, Nature 412(6845): 449--452 Ubiquitination-dependent mechanisms regulate synaptic growth and function. [FBrf0137327] Kraut et al., 2001, Curr. Biol. 11(6): 417--430 A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135708] Abdelilah-Seyfried et al., 2000, Genetics 155(2): 733--752 A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ. [FBrf0128367] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}fafEP381 Insertion

Dmel\P{EP}faf^EP381 Insertion