Dmel\P{EP}Taf1^EP421 Insertion

General Information
Symbol	Dmel\P{EP}Taf1^EP421	Species	D. melanogaster
Name		FlyBase ID	FBti0007821
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Taf1	Viability / fertility
Causes allele(s)	Taf1^EP421	Stock availability	1 publicly available
LINE ID	EP(3)0421
Genomic Location
Chromosomal location	3R ( 84A1 )	Sequence location	3R:2,472,764..2,472,764 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)	3 (BDGP Project Members, 1994-1999)
Sequence Location	3R:2,472,764..2,472,764 [+] (BDGP Project Members, 2000-)
Orientation	? (Wassarman et al., 2000)
Cytological location (computed by FlyBase)	84A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	84A2-84A2 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ025084 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Taf1 (FlyBase, 1992-, BDGP Project Members, 2000-)
Alleles and Phenotypes
Causes alleles	Taf1^EP421 (FlyBase, 1992-, Wassarman et al., 2000, Gene Disruption Project members, 2001-)

Lethality References viable (Wassarman et al., 2000)
Sterility References
Phenotype Manifest In
	egg chamber (Wassarman et al., 2000) eye photoreceptor cell (Wassarman et al., 2000) interommatidial bristle (Wassarman et al., 2000) macrochaeta (Wassarman et al., 2000) male terminalia (Wassarman et al., 2000) microchaeta (Wassarman et al., 2000) ocellus (Wassarman et al., 2000) oocyte (Wassarman et al., 2000) ovary (Wassarman et al., 2000) wing (Wassarman et al., 2000) wing vein L4 (Wassarman et al., 2000)
Detailed Description
Statement Reference Taf1^EP421/Taf1^XS-2232 flies have reduced bristle numbers on both male and female heads, are female sterile and are semi-lethal. They also display defects in oogenesis, ocelli development, and developmental timing. Ommatidia in Taf1^EP421/Taf1^XS-2232 flies are irregularly shaped and are not aligned in straight rows. The number of interommatidial bristles are reduced, multiple bristles sometimes originate from a single site and bristles are randomly distributed. The number of photoreceptor cells per ommatidium is also altered in these flies. Approximately 10% of ommatidia re missing one outer photoreceptor, and 14% have an extra outer photoreceptor. Taf1^EP421/Taf1^XS-2232 flies lack ocelli. Bristles on the thorax show normal morphology but are shifted from the normal orderly pattern. About 5% of Taf1^EP421/Taf1^XS-2232 males show an abnormal rotation of their terminalia during pupation. Wing development in Taf1^EP421/Taf1^XS-2232 is also disrupted. The wing vein L4 bifurcates along the wing margin forming a delta. The shape and size of the wings appear normal as does the morphology of the other wing veins. Wings are often held out to the sides of the body rather than crossed over the body as seen in wild-type. Taf1^EP421/Taf1^XS-2232 females are sterile with very small ovaries. Taf1^EP421/Taf1^S-625 mothers appear normal in size but eggs laid do not develop. Approximately 5% of egg chambers contain 8 instead of 16 cells. (Wassarman et al., 2000) Taf1^EP421/Taf1^S-136, Taf1^EP421/Taf1^S-625 flies are have reduced bristle numbers on both male and female heads and are female sterile. Taf1^EP421/Taf1^XS-2232 flies have reduced bristle numbers on both male and female heads, are female sterile and are semi-lethal - approximately a third of the expected progeny are seen. (Wassarman et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)0421 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17281 w¹¹¹⁸; P{EP}Taf1^EP421/TM3, Sb¹
Linkouts
Comments
	Location 3R:2472764-2472765 confirmed by FlyBase alignment of dbGSS accession AQ025084 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3)0421 (Wassarman et al., 2000, FlyBase, 1992-) ep(3)0421 (FlyBase, 1992-) P{EP}EP421 (FlyBase, 1992-) P{EP}Taf1^EP421 (FlyBase, 2005, FlyBase, 1992-) P{EP}Taf250^EP421 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Wassarman et al., 2000, Proc. Natl. Acad. Sci. USA 97(3): 1154--1159 TAF250 is required for multiple developmental events in Drosophila. [FBrf0125151] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{EP}Taf1EP421 Insertion

Dmel\P{EP}Taf1^EP421 Insertion