Dmel\P{EP}Pdk1^EP3091 Insertion

General Information
Symbol	Dmel\P{EP}Pdk1^EP3091	Species	D. melanogaster
Name		FlyBase ID	FBti0008008
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Pdk1	Viability / fertility
Causes allele(s)	Pdk1^EP3091	Stock availability	1 publicly available
LINE ID	EP(3)3091
Genomic Location
Chromosomal location	3L ( 61B1 )	Sequence location	3L:141,060..141,060 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)	3 (BDGP Project Members, 1994-1999)
Sequence Location	3L:141,060..141,060 [-] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	61B1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	61A4-61A4 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ025271 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Pdk1 (BDGP Project Members, 2000-)
Alleles and Phenotypes
Causes alleles	Pdk1^EP3091 (Cho et al., 2001, Rintelen et al., 2001, Gene Disruption Project members, 2001-, Schulz et al., 2004)

Lethality References lethal (Rintelen et al., 2001) lethal \| embryonic stage \| recessive (Cho et al., 2001) lethal \| larval stage \| recessive (Rintelen et al., 2001)
Sterility References
Phenotype Manifest In
	embryonic ventral epidermis (Cho et al., 2001) germline cell \| male (Schulz et al., 2004)
Detailed Description
Statement Reference Expression of Pk61C^EP3091 under the control of Scer\GAL4^ptc-559.1 (the animals are initially raised at 18^oC and then shifted to 29^oC after 10 days) results in a reduced number of early germ cells (stem cells, gonialblasts and spermatogonia) in the testis. (Schulz et al., 2004) Homozygotes produce no ventral cuticles and do not develop into the larval stage. Apoptotic activity is higher than controls in these embryos. (Cho et al., 2001) Eye size in animals expressing InR^Scer\UAS.cHa under the control of Scer\GAL4^GMR.PF is restored almost to wild type by Pk61C^EP3091/Pk61C^4ΔEP, although eye roughness is increased. (Rintelen et al., 2001) Homozygous and transheterozygous Pk61C⁵/Pk61C^EP3091 larvae die during the second instar. (Rintelen et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3091 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17260 w¹¹¹⁸; P{EP}Pdk1^EP3091
Linkouts
Comments
	Location 3L:124592-124593 confirmed by FlyBase alignment of dbGSS accession AQ025271 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3)3091 (Schulz et al., 2004, Rintelen et al., 2001, FlyBase, 1992-) ep(3)3091 (FlyBase, 1992-) P{EP}EP3091 (FlyBase, 1992-) P{EP}Pdk1^EP3091 P{EP}Pk61C^EP3091 (FlyBase, 1992-, FlyBase, 2005)
Secondary FlyBase IDs

References ( 11 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Schulz et al., 2004, Genetics 167(2): 707--723 A misexpression screen reveals effects of bag-of-marbles and TGF beta class signaling on the Drosophila male germ-line stem cell lineage. [FBrf0179436] Cho et al., 2001, Proc. Natl. Acad. Sci. USA 98(11): 6144--6149 Drosophila phosphoinositide-dependent kinase-1 regulates apoptosis and growth via the phosphoinositide 3-kinase-dependent signaling pathway. [FBrf0137097] Rintelen et al., 2001, Proc. Natl. Acad. Sci. USA 98(26): 15020--15025 PDK1 regulates growth through Akt and S6K in Drosophila. [FBrf0141724] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{EP}Pdk1EP3091 Insertion

Dmel\P{EP}Pdk1^EP3091 Insertion