Dmel\P{lacW}PRL-1^k09834 Insertion

General Information
Symbol	Dmel\P{lacW}PRL-1^k09834	Species	D. melanogaster
Name		FlyBase ID	FBti0008624
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	PRL-1	Viability / fertility	viable
Causes allele(s)	PRL-1^k09834	Stock availability	2 publicly available
LINE ID	l(2)k09834
Genomic Location
Chromosomal location	2L ( 35F1 )	Sequence location	2L:16,251,924..16,251,924 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{lacW}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800). Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)	2 (BDGP Project Members, 1994-1999)
Sequence Location	2L:16,251,924..16,251,924 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	35F1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	35F1-35F5 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025903 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	PRL-1 (Ashburner et al., 1999)
Alleles and Phenotypes
Causes alleles	PRL-1^k09834 (Ashburner et al., 1999, Spradling et al., 1999, Ashburner et al., 1999.10.12, Ashburner et al., 1999)

Lethality References viable (Ashburner et al., 1999, Spradling et al., 1999)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference No observable phenotypic effect. (Ashburner et al., 1999) No obvious phenotype. (Ashburner et al., 1999)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k09834 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	12178 y¹ w^67c23; P{lacW}PRL-1^k09834, l(2)k09834^k09834/CyO, P{sevRas1.V12}FK1
Kyoto	111434 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}PRL-1^k09834, l(2)k09834^k09834 P{neoFRT}40A/CyO y⁺
Availability	No longer maintained in the Berkeley P stock collection. (BDGP Project Members, 1994-1999)
Linkouts
Comments
	Location 2L:16247853-16247854 confirmed by FlyBase alignment of dbGSS accession AQ025903 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) Insertion within intron. (Ashburner et al., 1999) Insertion separable from lethal originally isolated on the same chromosome. (Spradling et al., 1999) Line not well characterized (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}k09834 (Ashburner et al., 1999, FlyBase, 1992-) P{lacW}PRL-1^k09834 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 12 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Ashburner et al., 1999, Genetics 153(1): 179--219 An exploration of the sequence of a 2.9-Mb region of the genome of Drosophila melanogaster. The Adh region. [FBrf0111326] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Ashburner et al., 1999, Genetics 153(1): Table S1. P-element insertions in the Adh region. [FBrf0112150]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Roote, 2000.6.13, New P derivatives. New P derivatives. [FBrf0129261] Ashburner et al., 1999.10.12, Adh. Adh. [FBrf0112153] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lacW}PRL-1k09834 Insertion

Dmel\P{lacW}PRL-1^k09834 Insertion