Dmel\P{PZ}spn-E^hls-03987 Insertion

General Information
Symbol	Dmel\P{PZ}spn-E^hls-03987	Species	D. melanogaster
Name		FlyBase ID	FBti0009068
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data
Affected gene(s)	Ecol\lacZ, spn-E	Viability / fertility
Causes allele(s)	Ecol\lacZ^spn-E-03987, spn-E^hls-03987	Stock availability	1 publicly available
LINE ID	fs(3)03987
Genomic Location
Chromosomal location	3R ( 89A5 )	Sequence location	3R:11,663,317..11,663,317 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	References Rouget et al., 2010, Nature 467(7319): 1128--1132
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:11,663,317..11,663,317 [+] (FlyBase, 2005) 3R:11,663,324..11,663,324 [+] (Gillespie and Berg, 1995)
Orientation
Cytological location (computed by FlyBase)	89A5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	89B1-89B2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ025574
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) spn-E (BDGP Project Members, 1994-1999, Gillespie and Berg, 1994, Rouget et al., 2010, Gillespie and Berg, 1995)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^spn-E-03987 (BDGP Project Members, 1994-1999) spn-E^hls-03987 (Gillespie and Berg, 1994, BDGP Project Members, 1994-1999, Gillespie and Berg, 1995, Spradling et al., 1999, Rouget et al., 2010)

Lethality References
Sterility References female sterile (Gillespie and Berg, 1994, Gillespie and Berg, 1995, Berg, 1994) female sterile \| recessive (Spradling et al., 1999) sterile (Gillespie and Berg, 1995)
Phenotype Manifest In
	dorsal appendage (Gillespie and Berg, 1994) egg chamber (Gillespie and Berg, 1994, Gillespie and Berg, 1995) meiosis & nuclear chromosome (Stapleton et al., 2001) oocyte (Gillespie and Berg, 1994, Gillespie and Berg, 1995)
Detailed Description
Statement Reference Suppressor of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon. (Pal-Bhadra et al., 2004) Nuage particles are lost in spn-E¹/spn-E^hls-03987 nurse cells and the perinuclear nuage is smoother than normal. (Snee and Macdonald, 2004) Homozygotes and trans-heterozygotes with spn-E¹ show an increased rate of non-disjunction in all except the small 4th chromosome. There are also substantial biases in recovery of reciprocal sperm classes. Nullo-XY sperm are recovered in considerable excess over the reciprocal XY sperm class. Also a weaker bias in favour of normal X-bearing sperm is seen. (Stapleton et al., 2001) Females lay few eggs and they appear to be unfertilised. Oocyte is not located at the usual posterior position within the egg, 95% of late stage egg chambers were ventralised and a small number of the egg chambers are degenerating. Heterozygotes with Df(3R)sbd105 (spn-E^-) exhibit mislocalised oocyte phenotype and degenerating egg chambers. Microtubule structure is disrupted in the egg chambers due to the inappropriate formation of an extensive microtubule meshwork during stage 8 to 9 (not premature cytoplasmic streaming). (Gillespie and Berg, 1995) 10% of eggs fail to localise the oocyte to the posterior of the egg chamber, 95% eggs have variable fused filaments. (Berg, 1994) Affects the early anteroposterior process in oogenesis. 5-10% of stage 2 to 10 eggs produced by homozygous female have mislocalised oocytes. 95% late stage egg chambers have a ventralised phenotype with only one respiratory appendage on the dorsal midline or two shortened appendages. No eggs hatch. (Gillespie and Berg, 1994) female-sterile 10% of eggs fail to localize oocyte to posterior of egg chamber; 95% of eggs have variable fused filaments.
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	fs(3)03987 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	24853 mwh¹ ry⁵⁰⁶ P{PZ}spn-E^hls-03987 e^s/TM3, ry^RK Sb¹
Linkouts
Comments
	Location 3R:11663316-11663317 determined by FlyBase alignment of dbGSS accession AQ025574 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	l(3)03987 (FlyBase, 1992-) P{PZ}fs(3)hls⁰³⁹⁸⁷ (Gillespie and Berg, 1994, FlyBase, 1992-) P{PZ}spn-E⁰³⁹⁸⁷ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{PZ}spn-E^hls-03987 (FlyBase, 2005, Gillespie and Berg, 1994, FlyBase, 1992-, Gillespie and Berg, 1995)
Secondary FlyBase IDs
	FBti0004815 FBti0009848
References ( 14 )
Research paper	Rouget et al., 2010, Nature 467(7319): 1128--1132 Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. [FBrf0212183] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Pal-Bhadra et al., 2004, Science 303(5658): 669--672 Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery. [FBrf0168038] Snee and Macdonald, 2004, J. Cell Sci. 117(10): 2109--2120 Live imaging of nuage and polar granules: evidence against a precursor-product relationship and a novel role for Oskar in stabilization of polar granule components. [FBrf0174811] Stapleton et al., 2001, Chromosoma 110(3): 228--240 A role of the Drosophila homeless gene in repression of Stellate in male meiosis. [FBrf0138260] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Gillespie and Berg, 1995, Genes Dev. 9(20): 2495--2508 Homeless is required for RNA localization in Drosophila oogenesis and encodes a new member of the DE-H family of RNA-dependent ATPases. [FBrf0083956] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Berg, 1994, [title not yet available] [title not yet available] [FBrf0075292]
Abstract	Gillespie and Berg, 1994, A. Dros. Res. Conf. 35: 109 fs(3)homeless, a member of the DE-H family of putative RNA-dependent ATPases, is required for oocyte localization and establishment of dorsoventral polarity during Drosophila oogenesis. [FBrf0067907]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{PZ}spn-Ehls-03987 Insertion

Dmel\P{PZ}spn-E^hls-03987 Insertion