FlyBase Insertion Report: Dmel\P{PZ}Hr38[02306]

General Information

Symbol

Dmel\P{PZ}Hr38⁰²³⁰⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0009115

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)02306

Affected gene(s)

anon-38E.8, Ecol\lacZ, Hr38

Viability / fertility

Associated allele(s)

Ecol\lacZ^Hr38-02306, Hr38⁰²³⁰⁶

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2L ( 38E3 )

Sequence Location

2L:20,714,623..20,714,624 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:20,714,623..20,714,624 [-]

(Kozlova et al., 1998)

Orientation

Cytological location (computed by FlyBase)

38E3 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2L:20714619..20714619 .

Sequence Data

Flanking sequence

G00581

(FlyBase, 1992-)

AJ001775

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

anon-38E.8

(Butler et al., 2001)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Kozlova et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Hr38

(Takayanagi-Kiya and Kiya, 2019, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Hr38-02306

Phenotypic Class Phenotype Manifest in Detailed Description

Hr38⁰²³⁰⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval anterior midgut

(Jackle and Janning, 1998.8.10)

embryonic/larval posterior midgut

(Jackle and Janning, 1998.8.10)

organism | segmentally repeated

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval peripheral nervous system | segmentally repeated

(Jackle and Janning, 1998.8.10)

larval stage & pupal stage & adult stage

epidermis

(Kozlova et al., 1998)

pharate adult stage P10 -- adult stage

proboscis

Comment: >=70 hr APF

(Kozlova et al., 1998)

wing | restricted

Comment: >=70 hr APF

(Kozlova et al., 1998)

Additional Information

Statement

Reference

Staining is first observed in the epidermis of feeding third instar larvae, becomes stronger in wandering third instar larvae and continues into pupal stages. In early pupae, homogeneous staining is seen. At about 70hr after puparium formation, staining becomes localized to the leg joints, proboscis, antennae, and the hinge region of the wing. The pattern persists in adults. At all stages the staining is mainly in the epidermis.

(Kozlova et al., 1998)

In embryo, expression in PNS not certain.

(Jackle and Janning, 1998.8.10)

In wandering third instar larva, no β-gal staining observed.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Hr38

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)02306

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

site of lethal P element insertion

(Kozlova et al., 1998)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1181

(Meister and Braun, 1995.10)

P{PZ}Hr38⁰²³⁰⁶

(Butler et al., 2001, Kozlova et al., 1998, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}l(2)02306⁰²³⁰⁶

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)02306⁰²³⁰⁶

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000291
FBti0005212

References (14)

Report Sections

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