A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{PZ}Hr3802306 Insertion

General Information
Symbol Dmel\P{PZ}Hr3802306 Species D. melanogaster
Name FlyBase ID FBti0009115
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) anon-38E.8, Ecol\lacZ, Hr38 Viability / fertility
Causes allele(s) Ecol\lacZHr38-02306, Hr3802306 Stock availability 1 publicly available
LINE ID l(2)02306
Genomic Location
Chromosomal location 2L ( 38E3 ) Sequence location 2L:20,714,623..20,714,624 [-]
Map ( GBrowse ) GBrowse View Help
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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Chromosome (arm)
Sequence Location
2L:20,714,623..20,714,624 [-]
(Kozlova et al., 1998)
2L:20,714,619..20,714,619 [+]
Orientation
Cytological location
(computed by FlyBase)
38E3 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
AJ001775
(FlyBase, 1992-)
G00581
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
anon-38E.8
(Butler et al., 2001)
Ecol\lacZ
(BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, Kozlova et al., 1998)
Hr38
(BDGP Project Members, 1994-1999, Kozlova et al., 1998)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZHr38-02306
(BDGP Project Members, 1994-1999, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, Kozlova et al., 1998)
Hr3802306
(BDGP Project Members, 1994-1999, Butler et al., 2001, Pokholkova et al., 1999, Kozlova et al., 1998, Spradling et al., 1999)
Lethality
References
lethal | adult stage | recessive
(Kozlova et al., 1998)
lethal | recessive
(Pokholkova et al., 1999, Spradling et al., 1999)
Sterility
References
hide Phenotype Manifest In
adult cuticle
(Pokholkova et al., 1999, Kozlova et al., 1998)
adult epidermis
(Kozlova et al., 1998)
joint
(Pokholkova et al., 1999)
hide Detailed Description
Statement
Reference
Homozygotes show local fragility of the adult cuticle; the cuticle in the leg joints is ruptured by mechanical stress, leading to melanisation of the damaged spots. The flies subsequently die as pharate adults or within a few hours after eclosion.
(Pokholkova et al., 1999)
Eclosing adults appear initially normal but die within a few hours following rupture of the cuticle at joints. Ruptures become melanized.
(Kozlova et al., 1998)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage  
anterior embryonic/larval midgut  
(Jackle and Janning, 1998.8.10)
posterior embryonic/larval midgut  
(Jackle and Janning, 1998.8.10)
organism | segmentally repeated  
(Jackle and Janning, 1998.8.10)
presumptive embryonic/larval peripheral nervous system | segmentally repeated  
(Jackle and Janning, 1998.8.10)
larval stage & pupal stage & adult stage  
epidermis  
(Kozlova et al., 1998)
pharate adult stage P10 -- adult stage  
proboscis  
(Kozlova et al., 1998)
Comment:>=70 hr APF
wing | restricted  
(Kozlova et al., 1998)
Comment:>=70 hr APF
Additional Information
Statement
Reference
In embryo, expression in PNS not certain.
(Jackle and Janning, 1998.8.10)
Staining is first observed in the epidermis of feeding third instar larvae, becomes stronger in wandering third instar larvae and continues into pupal stages. In early pupae, homogeneous staining is seen. At about 70hr after puparium formation, staining becomes localized to the leg joints, proboscis, antennae, and the hinge region of the wing. The pattern persists in adults. At all stages the staining is mainly in the epidermis.
(Kozlova et al., 1998)
In wandering third instar larva, no β-gal staining observed.
(Meister and Braun, 1995.10)
Marker for
Reflects
expression of
Hr38
(Meister and Braun, 1995.10)
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)02306
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11181
P{PZ}Hr3802306 cn1/CyO; ry506
hide Linkouts
hide Comments
site of lethal P element insertion
(Kozlova et al., 1998)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P1181
(Meister and Braun, 1995.10)
P{PZ}Hr3802306
(Butler et al., 2001, BDGP Project Members, 1994-1999, FlyBase, 1992-, Kozlova et al., 1998, )
P{PZ}l(2)0230602306
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{ry+t7.2=PZ}l(2)0230602306
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000291
  • FBti0005212
hide References ( 13 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Butler et al., 2001, Genetics 158(4): 1597--1614
Map position and expression of the genes in the 38 region of Drosophila. [FBrf0138405]
Pokholkova et al., 1999, D. I. S. 82: 56--58
Drosophila hormone receptor 38: phenotypic analysis of mutations generated by P-element excision. [FBrf0112007]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Kozlova et al., 1998, Genetics 149(3): 1465--1475
Drosophila hormone receptor 38 functions in metamorphosis. A role in adult cuticle formation. [FBrf0102956]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
Jackle and Janning, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103143]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]