A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{PZ}Hrb27C02647 Insertion

General Information
Symbol Dmel\P{PZ}Hrb27C02647 Species D. melanogaster
Name FlyBase ID FBti0009116
Feature type transposable_element_insertion_site
Description
Inserted element P{PZ} Expression data lacZ reporter
Affected gene(s) Ecol\lacZ, Hrb27C Viability / fertility
Causes allele(s) Ecol\lacZHrb27C-02647, Hrb27C02647 Stock availability 1 publicly available
LINE ID l(2)02647
Genomic Location
Chromosomal location 2L ( 27C4 ) Sequence location 2L:6,920,714..6,920,714 [+]
Map ( GBrowse ) detailed view FBti0111370 FBti0115469 FBti0112961 FBti0104584 FBti0102873 FBti0025936 FBti0104965 FBti0099950 FBti0111024 FBti0006937_1 FBti0038915 FBti0105578 FBti0108834 FBti0006937_2 FBti0102663 FBti0071106 FBti0033930 FBti0112192 FBti0109169 FBti0007688 FBti0077967 FBti0009116 FBti0057729 FBti0110482 FBti0028010 FBti0028011 FBti0027954 FBti0042346 FBti0028328 FBti0028889 FBti0124616 FBti0111435 FBti0026160 FBti0056090 FBti0105530 FBti0124617_2 FBti0124617_1 FBti0039453
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:6,920,714..6,920,714 [+]
(Levis R., 2009.03.30)
Orientation
+
(Levis R., 2009.03.30)
Cytological location
(computed by FlyBase)
27C4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073272
(FlyBase, 1992-,  
hide Inserted Element
Construct P{PZ}
Location-dependent
role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size 14.545Kb
Associated alleles
Ecol\lacZP\T.PZ
l(3)87Df7.2
ry+t7.2
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Hrb27C
(BDGP Project Members, 1994-1999)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZHrb27C-02647
(BDGP Project Members, 1994-1999, Vosshall, 1998.8.10)
Hrb27C02647
(BDGP Project Members, 1994-1999, Deal and Cook, 2005.7.11, Rottgen et al., 1998, Goodrich et al., 2004, Huynh et al., 2004, Yano et al., 2004, Price et al., 2000, Vorbruggen et al., 2000, Vorbruggen et al., 2000, Hammond et al., 1997, Campbell et al., 1995, Spradling et al., 1999)
Lethality
References
lethal
(Price et al., 2000, Huynh et al., 2004, Goodrich et al., 2004, Yano et al., 2004)
lethal | embryonic stage | recessive
(Campbell et al., 1995)
lethal | larval stage | recessive
(Hammond et al., 1997)
lethal | recessive
(Price et al., 2000, Spradling et al., 1999)
semi-lethal
(Yano et al., 2004)
viable
(Price et al., 2000)
Sterility
References
hide Phenotype Manifest In
egg
(Goodrich et al., 2004)
nurse cell & nuclear chromosome
(Goodrich et al., 2004)
oocyte & cytoskeleton | germ-line clone
(Yano et al., 2004)
wing margin
(Price et al., 2000)
hide Detailed Description
Statement
Reference
Dorsalisation of the eggs laid by sqd1/sqdK12 transheterozygotes is enhanced by Hrb27C02647/Hrb27C377 so that 100% show some degree of dorsalisation.
(Goodrich et al., 2004)
<2% of eggs laid by Hrb27C02647/Hrb27C377 females exhibit any degree of dorsalisation. Nurse cell chromosomes in Hrb27C377/Hrb27C02647 females do not disperse properly after stage 6 of oogenesis.
(Goodrich et al., 2004)
The penetrance and severity of embryonic cuticle patterning defects in osk3\'UTR.Scer\UAS.P\T; Scer\GAL4nos.UTR.T:Hsim\VP16 embryos is enhanced by Hrb27C02647/+.
(Yano et al., 2004)
The organisation and polarity of the oocyte cytoskeleton is somewhat abberant in these Hrb27C02647/Hrb27C02647 germline clones.
(Yano et al., 2004)
Hrb27Ck10413/Hrb27C02647 flies are viable but usually have small nicks in the wing margin.
(Price et al., 2000)
Mutation enhances the ectopic scutellar bristle phenotype seen from Scer\GAL4455.2-mediated expression of l(1)scScer\UAS.cHa.
(Rottgen et al., 1998)
hide Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage  
imaginal disc  
(Meister and Braun, 1995.10)
embryonic/larval brain  
(Meister and Braun, 1995.10)
gonad  
(Meister and Braun, 1995.10)
adult stage  
adult brain | restricted  
(Vosshall, 1998.8.10)
adult central complex | restricted  
(Vosshall, 1998.8.10)
Additional Information
Statement
Reference
In the adult, low level of expression in discrete region of the central brain.
(Vosshall, 1998.8.10)
Marker for
Reflects
expression of
Hrb27C
(Meister and Braun, 1995.10)
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)02647
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
11204
P{PZ}Hrb27C02647 cn1/CyO; ry506
hide Linkouts
hide Comments
Error in previous genomic location reported in FlyBase (2L:6,920,490, r5): original sequence location data reported for release 3 genomic sequence; FlyBase error in promotion to subsequent releases.
(Levis R., 2009.03.30)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
l(2)02647-5prime
(Levis R., 2009.03.30)
l(2)k02647
(Huynh et al., 2004)
P1204
(Meister and Braun, 1995.10)
P{PZ}0264702647
(FlyBase, 1992-)
P{PZ}Hrb27C02647
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}l(2)0264702647
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{PZ}wee02647
(FlyBase, 1992-)
P{ry+t7.2=PZ}0264702647
(FlyBase, 1992-)
P{ry+t7.2=PZ}wee02647
(FlyBase, 1992-)
Secondary FlyBase IDs
  • FBti0000751
  • FBti0003022
  • FBti0005221
hide References ( 19 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Goodrich et al., 2004, Development 131(9): 1949--1958
Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis. [FBrf0174571]
Huynh et al., 2004, Dev. Cell 6(5): 625--635
The Drosophila hnRNPA/B Homolog, Hrp48, Is Specifically Required for a Distinct Step in osk mRNA Localization. [FBrf0174543]
Yano et al., 2004, Dev. Cell 6(5): 637--648
Hrp48, a Drosophila hnRNPA/B Homolog, Binds and Regulates Translation of oskar mRNA. [FBrf0174544]
Price et al., 2000, Genetics 155(1): 159--166
Drosophila wee1 has an essential role in the nuclear divisions of early embryogenesis. [FBrf0127282]
Vorbruggen et al., 2000, Mech. Dev. 90(2): 305--308
Restricted expression and subnuclear localization of the Drosophila gene Dnop5, a member of the Nop/Sik family of the conserved rRNA processing factors. [FBrf0125275]
Vorbruggen et al., 2000, Mech. Dev. 90(2): 309--312
Localized expression of the Drosophila gene Dxl6, a novel member of the serine/arginine rich (SR) family of splicing factors. [FBrf0125277]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Rottgen et al., 1998, Molec. gen. Genet. 257(4): 442--451
A genetic screen for elements of the network that regulates neurogenesis in Drosophila. [FBrf0101998]
Hammond et al., 1997, Molec. Cell. Biol. 17(12): 7260--7267
Mutations in the hrp48 gene, which encodes a Drosophila heterogeneous nuclear ribonucleoprotein particle protein, cause lethality and developmental defects and affect P-element third-intron splicing in vivo. [FBrf0099247]
Campbell et al., 1995, Molec. Biol. Cell 6(10): 1333--1347
Drosophila Wee1 kinase rescues fission yeast from mitotic catastrophe and phosphorylates Drosophila Cdc2 in vitro. [FBrf0083814]
Mlodzik and Hiromi, 1992, Conn, 1992: 397--414
Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase
Levis R., 2009.03.30, Additional mapped insertions (release 3 coordinates).
Additional mapped insertions (release 3 coordinates). [FBrf0207436]
Deal and Cook, 2005.7.11, Isolation and characterization of Df(2L)BSC108.
Isolation and characterization of Df(2L)BSC108. [FBrf0188652]
Vosshall, 1998.8.10, Patterns of lacZ expression.
Patterns of lacZ expression. [FBrf0103142]
Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington.
lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714]
FlyBase analysis
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]