Dmel\P{PZ}gcm^rA87 Insertion

General Information
Symbol	Dmel\P{PZ}gcm^rA87	Species	D. melanogaster
Name		FlyBase ID	FBti0009197
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, gcm	Viability / fertility	viable
Causes allele(s)	Ecol\lacZ^gcm-rA87, gcm^rA87	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2L ( 30B12 )	Sequence location	2L:9,581,826..9,581,826 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	References Viktorin et al., 2011, Dev. Biol. 356(2): 553--565
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:9,581,826..9,581,826 [-] (Jones et al., 1995)
Orientation
Cytological location (computed by FlyBase)	30B12 ( inferred by FlyBase from sequence location )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Jones et al., 1995, Wildemann et al., 1997, Badenhorst, 2001, Gho et al., 1999, Bernardoni et al., 1999, De Iaco et al., 2006, Shandala et al., 2003, Yoshida et al., 2005, Bataille et al., 2005, Giangrande et al., 1993, Fossett et al., 2003, Lebestky et al., 2000, Lebestky et al., 2000, Granderath et al., 2000, Bernardoni et al., 1997, Soustelle et al., 2004, Udolph et al., 2001, Miller et al., 1998, Dye et al., 1998, Vincent et al., 1996, Jones et al., 2004, Giesen et al., 1997, Giangrande, 1996, Chotard et al., 2005, Soustelle and Giangrande, 2007, Paladi and Tepass, 2004, Jacques et al., 2009, Viktorin et al., 2011) gcm (Jones et al., 1995, Wildemann et al., 1997, Jones et al., 1995)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^gcm-rA87 (Jones et al., 1995, Wildemann et al., 1997, Badenhorst, 2001, Gho et al., 1999, Bernardoni et al., 1999, De Iaco et al., 2006, Shandala et al., 2003, Yoshida et al., 2005, Bataille et al., 2005, Giangrande et al., 1993, Fossett et al., 2003, Lebestky et al., 2000, Lebestky et al., 2000, Granderath et al., 2000, Bernardoni et al., 1997, Soustelle et al., 2004, Udolph et al., 2001, Miller et al., 1998, Dye et al., 1998, Vincent et al., 1996, Jones et al., 2004, Giesen et al., 1997, Giangrande, 1996, Chotard et al., 2005, Soustelle and Giangrande, 2007, Paladi and Tepass, 2004, Jacques et al., 2009, Viktorin et al., 2011) gcm^rA87 (Jones et al., 1995, Kammerer and Giangrande, 2001, Jones, 1999.11.22, Granderath et al., 2000, Vincent et al., 1996)

Lethality References lethal \| embryonic stage (Pereanu et al., 2007) viable (Jones et al., 1995, Vincent et al., 1996)
Sterility References
Phenotype Manifest In
	embryonic/larval ganglionic branch (Pereanu et al., 2007) glial cell (Pereanu et al., 2007) macrophage (Pereanu et al., 2007) sense organ & wing (Vincent et al., 1996) sense organ \| ectopic (Vincent et al., 1996) wing margin (Vincent et al., 1996)
Detailed Description
Statement Reference Loss of gcm in gcm[rA87] mutants results in embryonic lethality accompanied by the almost complete absence of glial cells and reduction in macrophages. The total loss of glia in gcm[rA87] mutant embryos causes neuronal pathfinding defects in stage 16 embryos. The cerebral trachea grows towards the dorsomedial surface of the neuropile normally. However, upon reaching the neuropile surface, it gives off numerous thick, long branches that are never observed in wild-type embryos. (Pereanu et al., 2007) Homozygotes exhibit an abnormal and variable number of ectopic sensory organs in the region of the twin sensillum on the wing margin (TSM). (Vincent et al., 1996) Transheterozygotes with gcm^- alleles are viable. (Jones et al., 1995)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage glial cell (Jones et al., 1995) interface glial cell (Jones et al., 1995) peripheral glial cell (Jones et al., 1995) head mesoderm (Jones et al., 1995) glial cell of peripheral nervous system (Jones et al., 1995) sense organ (Jones et al., 1995) embryonic stage \| late interface glial cell (Klaes et al., 1994) CNS glial cell (Klaes et al., 1994) embryonic stage 5 (Jones et al., 1995) embryonic stage 8 head mesoderm (Bernardoni et al., 1997) embryonic stage 9 -- 17 embryonic/larval hemocyte (Bernardoni et al., 1997) embryonic stage 11 embryonic brain (Jones et al., 1995) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage neuroglioblast (Bernardoni et al., 1999)
Additional Information
Statement Reference Enhancer trap expression is observed at embryonic stage 8 in the procephalic mesoderm. Later, it is observed in all hemocytes. (Bernardoni et al., 1997) Enhancer trap expression first occurs in an anterior ventral patch in the cellular blastoderm embryo. During gastrulation, these cells invaginate at the end of the ventral furrow just anterior to the cephalic furrow in an area that gives rise to the cephalic mesoderm. During stages 11-12, in each hemisegment, sequential and transient expression occurs in two patches of 3-5 ectodermal cells. From each patch, a single large blast cell delaminates and maintains expression. These are tentatively identified as the peripheral glioblast and the longitudinal glioblast. By late stage 11, enhancer trap expression occurs in all of the progeny of these glioblasts as well as in additional glial cells. In the brain lobes, a complex pattern of expression is seen through stage 17. In the PNS, expression occurs in all repo-positive glia along peripheral axon pathways and associated with sensory organs. (Jones et al., 1995) By late stage 11, enhancer trap expression occurs in all of the progeny of these glioblasts as well as in additional glial cells. In the brain lobes, a complex pattern of expression is seen through stage 17. In the PNS, expression occurs in all repo-positive glia along peripheral axon pathways and associated with sensory organs. (Jones et al., 1995)

Marker for	glial cell (Badenhorst, 2001)
Reflects expression of	gcm (Jones et al., 1995)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	5445 P{PZ}gcm^rA87/CyO; ry⁵⁰⁶
Linkouts
Comments
	Position based on author statement that insertion is 109bp upstream of longest cDNA in FBrf0084071. (Jones et al., 1995)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	gcm-lacZ (Lebestky et al., 2000, Lebestky et al., 2000, Paladi and Tepass, 2004, Viktorin et al., 2011) gcm-lacZ(gcm^rA87) (Bataille et al., 2005) gcm-lacZ^r87 (Yoshida et al., 2005) gcm-lacZ^rA87 (Udolph et al., 2001, Viktorin et al., 2011) P{A92}gcm^rA87 (FlyBase, 1992-) P{A92}rA87 (FlyBase, 1992-) P{lacZ-un1}gcm^rA87 (Wildemann et al., 1997, FlyBase, 1992-) P{PZ}gcm^rA87 (FlyBase, 1992-, Jones et al., 1995) P{ry⁺t7.2=PZ}gcm^rA87 (Paladi and Tepass, 2004) rA87 (De Iaco et al., 2006, Soustelle et al., 2004, Shandala et al., 2003, Badenhorst, 2001, Bernardoni et al., 1999, Gho et al., 1999, Klaes et al., 1994, Chotard et al., 2005, Soustelle and Giangrande, 2007, Jacques et al., 2009)
Secondary FlyBase IDs
	FBti0002155
References ( 34 )

Generate a list of	All references relating to this insertion ( 34 )

List References by type	Research paper ( 30 ) Supplementary material ( 1 ) Review ( 1 ) Personal communication to FlyBase ( 1 ) FlyBase analysis ( 1 )
Recent research papers ( 1 )
	Viktorin et al., 2011, Dev. Biol. 356(2): 553--565 Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development. [FBrf0214495] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...

Dmel\P{PZ}gcmrA87 Insertion

Dmel\P{PZ}gcm^rA87 Insertion