Dmel\P{lacW}Mvl^97f Insertion

General Information
Symbol	Dmel\P{lacW}Mvl^97f	Species	D. melanogaster
Name		FlyBase ID	FBti0009226
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, Mvl	Viability / fertility
Causes allele(s)	Ecol\lacZ^Mvl-97f, Mvl^97f	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	3R ( 93B7-93B8 )	Sequence location
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	93B7-93B8 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Rodrigues et al., 1995) Mvl (Rodrigues et al., 1995, Bettedi et al., 2011, Rose et al., 2011)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^Mvl-97f (Rodrigues et al., 1995) Mvl^97f (Rodrigues et al., 1995, D'Souza et al., 1999, Bloomington Drosophila Stock Center, 1999.7.1, Bettedi et al., 2011, Rose et al., 2011)

Lethality References lethal \| conditional qualifier (Southon et al., 2008) semi-lethal \| conditional qualifier \| female limited (Southon et al., 2008)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Mvl[97f] mutants exhibit an iron-deficient phenotype, but display comparable levels of copper, manganese and zinc to wild-type controls. A dietary supplement of 1 mmol/l CuSO[[4]] leads to a decrease in total body iron, yet these flies accumulate iron to similar levels as controls. Mvl[97f] mutants accumulate less iron in ferritin than controls, except when fed on an iron-enriched food. There is a decrease in iron-loaded ferritin content following dietary copper supplementation in Mvl[97f] flies. Dietary iron accumulates primarily in the anterior midgut and copper in the diet reduces iron content of the middle midgut. Intestines from Mvl[97f] homozygotes show severely reduced iron content in the middle midgut. Dietary iron fails to rescue iron storage in this region, whereas in the anterior midgut ferritin induction and iron accumulation are normal. The addition of copper to the diet reduces iron content in Mvl[97f] intestines to below levels of histochemical detection. (Bettedi et al., 2011) There is a 4-log reduction in viral titers of flies that are infected with Sindbis virus in Mvl[97f] mutants compared with heterozygous or wild-type controls, while vesicular stomatitis virus (VSV) infection is unaffected. (Rose et al., 2011) Mvl[97f] flies show no significant changes in pigmentation or cuticle morphology under basal, copper-limited, or copper-excessive conditions. Mvl[97f] flies show a strong sensitivity to excess copper, with significant reduced viability at 1 mmol 1[-1] Cu and essentially no survival at 2 mmol 1[-1] Cu. Under limiting Cu conditions Mvl[97f] females demonstrate reduced viability, but males are unaffected. The pupae of Mvl[97f] larvae raised on basal food show 40% lower iron levels than controls. Copper, Zinc and Manganese levels are not significantly lower. Larvae raised under copper-limited conditions show significantly reduced copper levels in both Mvl[97f] and control pupae. Mvl[97f] larvae raised under basal conditions and exposed to 6 mmol 1[-] copper for a 24 hour period as adults show dramatically higher levels of copper. These flies also contain significantly more manganese and zinc than controls, and iron levels are restored. (Southon et al., 2008) ATP7[Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[pnr-MD237] in a Mvl[97f]/+ background does not significantly affect pigmentation or thoracic development compared to overexpression in a wildtype background. (Southon et al., 2008) The defects shown by Mvl^97f flies in a feeding preference assay are partially rescued, in a dose-dependent manner, by expression of Hsap\NRAMP1^hs.PD when flies are reared at 25^oC or 27^oC. (D'Souza et al., 1999) Mvl^97f flies show defects in a feeding preference assay; only 30% of the Mvl^97f flies preferentially choose sucrose (in a choice between agar containing 1 mmol l^-1 sucrose and control agar) in contrast to 90% of normal flies. (D'Souza et al., 1999) Mutants do not discriminate between tastes, showing no preference for sugar supplemented medium, unlike wild type flies. Addition of Mn²⁺ and Fe²⁺ reverses the mutant phenotype in a dose-dependent manner. Addition of Ca²⁺, Mg²⁺, or Zn²⁺ fails to revert the phenotype. Metal ions are not required for correct development to the nervous system components involved in taste perception: transfer of newly eclosed adults onto Mn²⁺ Fe²⁺ supplemented medium does not rescue the mutant taste phenotype. Mn²⁺ and Fe²⁺ are implicated in a signal transduction pathway underlying taste perception. (Orgad et al., 1998) Heterozygotes and homozygotes show a reduced preference for several sugars and an increased acceptance of low concentrations of sodium chloride, though homozygotes show a significantly stronger phenotype. Homozygotes show a shift in the threshold of acceptance of sucrose, fructose and trehalose, as measured in the feeding preference test and the proboscis extension test. Higher concentrations of stimulus are required to elicit a maximal behavioral response. (Rodrigues et al., 1995)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10 procephalic segment (Rodrigues et al., 1995) tracheal pit \| vicinity of (Rodrigues et al., 1995) embryonic stage 16 antenno-maxillary complex (Rodrigues et al., 1995) amnioserosa (Rodrigues et al., 1995) pupal stage macrophage (Rodrigues et al., 1995) adult stage pseudotracheal sensillum (Rodrigues et al., 1995) optic lobe (Rodrigues et al., 1995) sensory neuron of third segment of antenna (Rodrigues et al., 1995) macrophage (Rodrigues et al., 1995) adult brain (Rodrigues et al., 1995) proboscis (Rodrigues et al., 1995) labellar taste bristle (Rodrigues et al., 1995) sensory neuron (Rodrigues et al., 1995) maxillary palp sense organ (Rodrigues et al., 1995)
Additional Information
Statement Reference Enhancer trap expression is observed in mature neruons in the central and peripheral nervous systems in the adult. Specifically, staining is observed in sensory neurons on the third antennal segment, the proboscis, and the maxillary palps. Staining is also observed in macrophages that are associated with a number of structures throughout development. In the embryo, staining is observed in germband extended embryos in cells surrounding the tracheal pits and in the procephalic lobe. Staining is observed along the posterior border of segment boundaries during germband retraction. Staining also occurs in the amnioserosa and is observed as two rows of cells during dorsal closure. Staining is observed in mature neurons of the embryonic central and peripheral nervous systems and is most prominent in the cells of the antenno-maxillary complex. (Rodrigues et al., 1995)

Marker for
Reflects expression of	Mvl (Rodrigues et al., 1995)
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	5151 w¹¹¹⁸; P{lacW}Mvl^97f
Linkouts
Comments
	Verified that insertion is P{lacW}. (FBrf0108531, Bloomington Drosophila Stock Center, 1999.7.1)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}Mvl⁹⁷ (FlyBase, 1992-) P{lacW}Mvl^97f (FlyBase, 1992-, Bettedi et al., 2011) P{lacZ-97f}Mvl^97f (FlyBase, 1992-) P{lacZ-un3}Mvl^97f (Rodrigues et al., 1995, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0012917
References ( 10 )
Research paper	Bettedi et al., 2011, J. Exp. Biol. 214(6): 971--978 Iron depletion in the intestines of Malvolio mutant flies does not occur in the absence of a multicopper oxidase. [FBrf0213129] Rose et al., 2011, Cell Host Microbe 10(2): 97--104 Natural resistance-associated macrophage protein is a cellular receptor for sindbis virus in both insect and Mammalian hosts. [FBrf0214675] Southon et al., 2008, J. exp. Biol. 211(5): 709--716 Malvolio is a copper transporter in Drosophila melanogaster. [FBrf0203166] D'Souza et al., 1999, J. exp. Biol. 202(14): 1909--1915 Functional complementation of the malvolio mutation in the taste pathway of Drosophila melanogaster by the human natural resistance-associated macrophage protein 1 (Nramp-1). [FBrf0108689] Orgad et al., 1998, J. exp. Biol. 201(1): 115--120 Metal ions suppress the abnormal taste behavior of the Drosophila mutant malvolio. [FBrf0100379] Rodrigues et al., 1995, EMBO J. 14(13): 3007--3020 malvolio, the Drosophila homologue of mouse NRAMP-1 (Bcg), is expressed in macrophages and in the nervous system and is required for normal taste behaviour. [FBrf0082530] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 1999.7.1, P{w<up>+mC</up>=lacW}Mvl<up>97</up>. P{w^+mC=lacW}Mvl⁹⁷. [FBrf0108616] [FBrf0108531]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{lacW}Mvl97f Insertion

Dmel\P{lacW}Mvl^97f Insertion