Dmel\P{lArB}14-3-3ζ^P1188 Insertion

General Information
Symbol	Dmel\P{lArB}14-3-3ζ^P1188	Species	D. melanogaster
Name		FlyBase ID	FBti0009360
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lArB}	Expression data	lacZ reporter
Affected gene(s)	14-3-3ζ, Ecol\lacZ	Viability / fertility
Causes allele(s)	14-3-3ζ^P1188, Ecol\lacZ^{14-3-3ζ-P1188}	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	2R ( 46E6-46E8 )	Sequence location
Member of Large Scale Dataset(s)
Dataset
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	46E6-46E8 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lArB}
Location-dependent role	lacZ enhancer trap (Wilson et al., 1989)
Size	18.327Kb
Associated alleles	Adh^+t3.2 Ecol\lacZ^P\T.A92a ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	14-3-3ζ (Han et al., 1996) Ecol\lacZ (Han et al., 1996, Skoulakis and Davis, 1996)
Alleles and Phenotypes
Causes alleles	14-3-3ζ^P1188 (Han et al., 1996, Su et al., 2001, Philip et al., 2001, Benton et al., 2002, Broadie et al., 1997, Skoulakis and Davis, 1996) Ecol\lacZ^{14-3-3ζ-P1188} (Han et al., 1996, Skoulakis and Davis, 1996)

Lethality References lethal (Philip et al., 2001, Benton et al., 2002, Messaritou et al., 2009, Messaritou et al., 2010) lethal \| embryonic stage \| recessive (Skoulakis and Davis, 1996) lethal \| recessive (Broadie et al., 1997, Messaritou et al., 2010) viable (Philip et al., 2001)
Sterility References
Phenotype Manifest In
	border follicle cell \| somatic clone (McDonald et al., 2008) denticle belt \| germline clone (Li et al., 1997) embryonic/first instar larval cuticle \| germline clone (Li et al., 1997) follicle cell \| somatic clone (Benton and St. Johnston, 2003) syncytial blastoderm embryo \| germline clone (Li et al., 1997)
Detailed Description
Statement Reference Expression of 14-3-3ζ[I.Scer\UAS] under the control of Scer\GAL4[elav.PLu] partially rescues the lethality found in 14-3-3ζ[P1188] homozygotes and 14-3-3ζ[P1188]/14-3-3ζ[P2335] to approximately 20% of expected. Expression of 14-3-3ζ[II.Scer\UAS] under the control of Scer\GAL4[elav.PLu] partially rescues the lethality found in 14-3-3ζ[P1188] homozygotes and 14-3-3ζ[P1188]/14-3-3ζ[P2335] heterozygotes to approximately 15-20% of expected. Expression of 14-3-3ζ[III.Scer\UAS.T:Zzzz\FLAG] under the control of Scer\GAL4[elav.PLu] partially rescues the lethality found in 14-3-3ζ[P1188] homozygotes and 14-3-3ζ[P1188]/14-3-3ζ[P2335] heterozygotes to approximately 60-70% of expected. (Messaritou et al., 2009) 12-18% of 14-3-3ζ[P1188]/14-3-3ζ[P2335] heteroallelic animals are escapers. They do not exhibit external defects or grossly aberrant behaviors, despite no 14-3-3ζ expression. (Messaritou et al., 2009) Border follicle cell migration is disrupted in mosaic egg chambers containing homozygous follicle cell clones. (McDonald et al., 2008) Follicular epithelium morphogenesis is normal in 14-3-3ζ^P1188/14-3-3ζ^P1375 ; 14-3-3ε^j2B10/+ egg chambers up to stage 4, but the follicle cells subsequently lose their regular cuboidal shape. (Benton and St. Johnston, 2003) Large homozygous follicle cell clones show dramatic defects in tissue organisation, forming multilayers of morphologically abnormal cells that often fail to encapsulate germline cysts properly. Smaller homozygous follicle cell clones that arise after epithelium formation have milder and less penetrant defects in morphology and polarity. (Benton and St. Johnston, 2003) Many 14-3-3ζ^P1188 germ-line clones in 14-3-3ε^j2B10/+ females have defects in oocyte specification and polarization. Many 14-3-3ζ^P1188 germ-line clones in 14-3-3ε^S-1259/+ females have defects in oocyte specification. The penetrance of the oocyte to nurse cell transformation phenotype seen in 14-3-3ε^j2B10 germ-line clones (80% n=106) is dominantly enhanced to 100% by 14-3-3ζ^P1188. (Benton et al., 2002) 14-3-3ζ^P1188 germ-line clones do not exhibit defects in oocyte specification or polarization. (Benton et al., 2002) Flies rescued from lethality by 14-3-3ζ^LI.15.hs or 14-3-3ζ^LI.15.hs and 14-3-3ζ^LII.2.hs show a 25-30% decrement in olfactory learning, comparable to that of (rescued) 14-3-3ζ^2.3. The restoration of learning by the transgenes decays back to mutant levels 60-70 hr later. (Philip et al., 2001) Homozygous embryos show no apparent defects in the timing of mitotic cycle 14 and show delayed mitosis after irradiation (as occurs in wild type). 69 +/- 9% of mutant embryos derived from homozygous female germline clones fail to cellularise. 54/59 of the embryos have defects in cell division, including DNA bridges between telophase sister nuclei, asynchrony in division within a single embryo, free microtubule-organizing centres that are not associated with nuclei, loss of nuclei from the cortical monolayer of nuclei and larger than normal yolk DNA masses. Chromosome bridges interconnecting DNA masses are seen as early as telophase of the fourth embryonic mitosis. Mitotic spindles do appear to be formed in these embryos (as judged by the segregation of chromosome masses that are still linked by DNA bridges to opposite spindle poles), and attempts at the formation of mid-bodies are seen between segregating nuclei, despite the presence of chromosome bridges. Approximately 30% of embryos cellularise. These embryos have severe gastrulation defects. (Su et al., 2001) Embryos do not exhibit a dorsal closure defect. The amplitude and frequency of endogenous excitatory junctional currents (EJCs) is reduced relative to wild type and the NMJ exhibits a transmission defect, the calcium dependence curve is shifted to the right indicating a higher level of external calcium is required to achieve the given level of secretion. (Broadie et al., 1997) Embryos derived from homozygous female germline clones show a variable phenotype that is not significantly different whether or not the embryos contain a wild-type copy of 14-3-3ζ from the father. Approximately 50% of the embryos do not develop cuticles, and the remainder develop cuticles with various segmentation defects including missing and/or fused denticle bands. The Filzkorper appear normal. Approximately 50% of the embryos appear to stop development during the syncytial blastoderm stage, and contain many fewer nuclei compared to wild-type. Some of these nuclei appear fused. 18% of embryos carrying 14-3-3ζ^hbNRE.RpII15 and derived from homozygous 14-3-3ζ^P1188 female germline clones have a wild-type anterior region but are missing all or part of the posterior region. 56% of these \'anteriorly rescued\' embryos have shortened Filzkorper. (Li et al., 1997) Heterozygotes exhibit normal 3 minute memory performance. Odour avoidance (octanol and benzaldehyde) is normal. (Skoulakis and Davis, 1996)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference adult stage glial cell of medulla (Skoulakis and Davis, 1996) thoracico-abdominal ganglion (Skoulakis and Davis, 1996) adult antennal lobe (Skoulakis and Davis, 1996) adult mushroom body (Skoulakis and Davis, 1996)
Additional Information
Statement Reference Intense staining is observed in the perikarya of mushroom bodies. Staining is also observed in the cortex of the antennal lobe, the thoracic ganglia, and in apparent glial cells of the medulla. (Skoulakis and Davis, 1996)

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	9402 P{lArB}14-3-3ζ^P1188/CyO; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lArB}14-3-3^P1188 (Han et al., 1996, FlyBase, 1992-) P{lArB}14-3-3ζ^P1188 (Han et al., 1996, FlyBase, 1992-) P{lArB}leo^P1188 (Han et al., 1996, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0004088 FBti0004222
References ( 13 )
Research paper	Messaritou et al., 2010, J. Biol. Chem. 285(3): 1692--1700 Dimerization is essential for 14-3-3zeta stability and function in vivo. [FBrf0209680] Messaritou et al., 2009, FEBS Lett. 583(17): 2934--2938 A third functional isoform enriched in mushroom body neurons is encoded by the Drosophila 14-3-3zeta gene. [FBrf0208682] McDonald et al., 2008, Curr. Biol. 18(21): 1659--1667 PAR-1 Kinase Regulates Epithelial Detachment and Directional Protrusion of Migrating Border Cells. [FBrf0206227] Benton and St. Johnston, 2003, Cell 115(6): 691--704 Drosophila PAR-1 and 14-3-3 inhibit Bazooka/PAR-3 to establish complementary cortical domains in polarized cells. [FBrf0173208] Benton et al., 2002, Dev. Cell 3(5): 659--671 Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation. [FBrf0151902] Philip et al., 2001, J. Neurosci. 21(21): 8417--8425 Conditional rescue of olfactory learning and memory defects in mutants of the 14-3-3 gene leonardo. [FBrf0139731] Su et al., 2001, J. Cell Sci. 114(19): 3445--3454 Cell cycle roles for two 14-3-3 proteins during Drosophila development. [FBrf0139721] Broadie et al., 1997, Neuron 19(2): 391--402 Leonardo, a Drosophila 14-3-3 protein involved in learning, regulates presynaptic function. [FBrf0098203] Li et al., 1997, Development 124(20): 4163--4171 The Drosophila 14-3-3 protein Leonardo enhances Torso signaling through D-Raf in a Ras 1-dependent manner. [FBrf0099365] Han et al., 1996, J. Neurobiol. 31(1): 88--102 The Drosophila brain revisited by enhancer detection. [FBrf0089671] Skoulakis and Davis, 1996, Neuron 17(5): 931--944 Olfactory learning deficits in mutants for leonardo, a Drosophila gene encoding a 14-3-3 protein. [FBrf0090801] Wilson et al., 1989, Genes Dev. 3(9): 1301--1313 P-element-mediated enhancer detection: an efficient method for isolating and characterizing developmentally regulated genes in Drosophila. [FBrf0049802]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{lArB}14-3-3ζP1188 Insertion

Dmel\P{lArB}14-3-3ζ^P1188 Insertion