Dmel\P{PZ}l(3)10532¹⁰⁵³² Insertion

General Information
Symbol	Dmel\P{PZ}l(3)10532¹⁰⁵³²	Species	D. melanogaster
Name		FlyBase ID	FBti0009535
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, l(3)10532	Viability / fertility
Causes allele(s)	Ecol\lacZ¹⁰⁵³², l(3)10532¹⁰⁵³²	Stock availability	none publicly available
LINE ID	l(3)10532
Genomic Location
Chromosomal location	3L ( 73A2 )	Sequence location	3L:16,476,761..16,476,761 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:16,476,761..16,476,761 [+]
Orientation
Cytological location (computed by FlyBase)	73A2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	73A1-73A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	G00603 (FlyBase, 1992-,
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Meister and Braun, 1995.10, Treisman and Rubin, 1996) l(3)10532 (Treisman and Rubin, 1996)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ¹⁰⁵³² (Meister and Braun, 1995.10, Treisman and Rubin, 1996) l(3)10532¹⁰⁵³² (Treisman and Rubin, 1996, BDGP Project Members, 1994-1999)

Lethality References lethal \| recessive (Treisman and Rubin, 1996)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference third instar larval stage photoreceptor cell \| presumptive (Treisman and Rubin, 1996) eye disc (Treisman and Rubin, 1996) wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval brain (Meister and Braun, 1995.10) gonad (Meister and Braun, 1995.10)
Additional Information
Statement Reference In third instar eye disc, expressed in all photoreceptors. (Treisman and Rubin, 1996) In wandering third instar. weak β-gal staining in the larval brain and gonad; very weak staining in imaginal discs. (Which imaginal discs not specified.) (Meister and Braun, 1995.10)

Marker for
Reflects expression of	l(3)10532 (Meister and Braun, 1995.10)
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)10532 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	3-10532 (Treisman and Rubin, 1996) P2076 (Meister and Braun, 1995.10) P{PZ}l(3)10532¹⁰⁵³² (Treisman and Rubin, 1996, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Treisman and Rubin, 1996, Mech. Dev. 56(1-2): 17--24 Targets of glass regulation in the Drosophila eye disc. [FBrf0089138] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] Meister and Braun, 1995.10, lacZ expression patterns for P{} insertions at Bloomington. lacZ expression patterns for P{} insertions at Bloomington. [FBrf0083714] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{PZ}l(3)1053210532 Insertion

Dmel\P{PZ}l(3)10532¹⁰⁵³² Insertion