Dmel\P{lacW}Hrb27C^k02814 Insertion

General Information
Symbol	Dmel\P{lacW}Hrb27C^k02814	Species	D. melanogaster
Name		FlyBase ID	FBti0009612
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Hrb27C	Viability / fertility
Causes allele(s)	Ecol\lacZ^{Hrb27C-k02814}, Hrb27C^k02814	Stock availability	2 publicly available
LINE ID	l(2)k02814
Genomic Location
Chromosomal location	2L ( 27C4 )	Sequence location
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	27C4 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Hrb27C (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^{Hrb27C-k02814} (BDGP Project Members, 1994-1999) Hrb27C^k02814 (BDGP Project Members, 1994-1999, Goodrich et al., 2004, Huynh et al., 2004, Yano et al., 2004, Spradling et al., 1999)

Lethality References lethal (Goodrich et al., 2004, Yano et al., 2004, Huynh et al., 2004) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References
Phenotype Manifest In
	dorsal appendage \| germline clone (Goodrich et al., 2004) egg \| germline clone (Goodrich et al., 2004) egg operculum \| germline clone (Goodrich et al., 2004) oocyte & cytoskeleton \| germ-line clone (Yano et al., 2004) ovary \| germline clone (Yano et al., 2004)
Detailed Description
Statement Reference The eggs laid by Hrb27C^k02814 germ-line clone carrying females exhibit varying degrees of dorsalisation: least affect have widely spaced dorsal appendage and an enlarged operculum; intermediately affected have a single broad appendage that spans at least the width of the normal appendages; severely affected have a crown of appendage material that surrounds the anterior circumference of the egg. Most of the eggs laid are abnormally short. (Goodrich et al., 2004) The penetrance and severity of embryonic cuticle patterning defects in osk^{3\'UTR.Scer\UAS.P\T}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} embryos is enhanced by Hrb27C^k02814/+. (Yano et al., 2004) The majority of Hrb27C^k02814 germline clones fail to develop beyond the early stages of oogenesis. The organisation and polarity of the oocyte cytoskeleton is somewhat abberant in these germline clones. (Yano et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(2)k02814 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10375 y¹ w^67c23; P{lacW}Hrb27C^k02814/CyO
Kyoto	111072 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Hrb27C^k02814 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu). (FlyBase, 1992-)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}Hrb27C^k02814 (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{lacW}l(2)02647^k02814 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs
	FBti0007038
References ( 9 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Goodrich et al., 2004, Development 131(9): 1949--1958 Hrb27C, Sqd and Otu cooperatively regulate gurken RNA localization and mediate nurse cell chromosome dispersion in Drosophila oogenesis. [FBrf0174571] Huynh et al., 2004, Dev. Cell 6(5): 625--635 The Drosophila hnRNPA/B Homolog, Hrp48, Is Specifically Required for a Distinct Step in osk mRNA Localization. [FBrf0174543] Yano et al., 2004, Dev. Cell 6(5): 637--648 Hrp48, a Drosophila hnRNPA/B Homolog, Binds and Regulates Translation of oskar mRNA. [FBrf0174544] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Hrb27Ck02814 Insertion

Dmel\P{lacW}Hrb27C^k02814 Insertion