Dmel\P{PZ}eIF5B⁰⁹¹⁴³ Insertion

General Information
Symbol	Dmel\P{PZ}eIF5B⁰⁹¹⁴³	Species	D. melanogaster
Name		FlyBase ID	FBti0009847
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	Ecol\lacZ, eIF5B, sty	Viability / fertility	viable
Causes allele(s)	Ecol\lacZ^sty-09143, eIF5B⁰⁹¹⁴³	Stock availability	1 publicly available
LINE ID	l(3)09143
Genomic Location
Chromosomal location	3L ( 63E1 )	Sequence location	3L:3,450,279..3,450,279 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{PZ}.BDGP A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue. Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection. (Spradling et al., 1999, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:3,450,279..3,450,279 [+]
Orientation
Cytological location (computed by FlyBase)	63E1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	63D1--62D2 (in situ hybridization reported) (Samakovlis et al., 1996) 63D1--63D2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	G00521 (FlyBase, 1992-)
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (Franzdóttir et al., 2009, Samakovlis et al., 1996, Iwanami et al., 2005, Steneberg et al., 1998, Sen et al., 2004, Englund et al., 1999, Casci et al., 1999, Kramer et al., 1999, Imam et al., 1999, FlyBase, 1996-, Vosshall, 1998.8.10, Lee and Treisman, 2001, Hacohen et al., 1998, Pallavi et al., 2006) eIF5B (FlyBase, 1996-) sty (Hacohen et al., 1998, BDGP Project Members, 1994-1999, Samakovlis et al., 1996)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^sty-09143 (Samakovlis et al., 1996, Iwanami et al., 2005, Steneberg et al., 1998, Franzdóttir et al., 2009, Sen et al., 2004, Englund et al., 1999, Casci et al., 1999, Kramer et al., 1999, Imam et al., 1999, FlyBase, 1996-, Vosshall, 1998.8.10, Lee and Treisman, 2001, Hacohen et al., 1998, Pallavi et al., 2006) eIF5B⁰⁹¹⁴³ (FlyBase, 1996-)

Lethality References viable (Hacohen et al., 1998)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 12 -- 17 embryonic/larval tracheal system \| restricted (Samakovlis et al., 1996) embryonic stage 14 embryonic/larval dorsal branch 1 (Samakovlis et al., 1996) embryonic/larval dorsal branch 2 (Samakovlis et al., 1996) wandering third instar larval stage imaginal disc (Meister and Braun, 1995.10) embryonic/larval trachea (Meister and Braun, 1995.10) pupal stage P5(ii) antennal disc \| restricted (Sen et al., 2004) Comment:reference states 16 hr APF adult stage optic lobe (Vosshall, 1998.8.10)
Additional Information
Statement Reference Expression in the pupal antennal disc does not overlap the region of ato expression. (Sen et al., 2004) In embryonic tracheal system: at stage 12, expressed in many or all cells of each budding primary branch; expression gradually restricted to cells at the leading ends. (Samakovlis et al., 1996)

Marker for	pupal antenna disc (Sen et al., 2004)
Reflects expression of	sty (Sen et al., 2004)
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)09143 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	11735 P{PZ}eIF5B⁰⁹¹⁴³, l(3)09143⁰⁹¹⁴³ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹
Linkouts
Comments
	Insertion is separable from lethal l(3)09143. (Hacohen et al., 1998)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P1735 (Sen et al., 2004, Meister and Braun, 1995.10) P(PZ)eIF5B⁰⁹¹⁴³ (Franzdóttir et al., 2009) P{PZ}09143 (BDGP Project Members, 1994-1999, Samakovlis et al., 1996, FlyBase, 1992-) P{PZ}eIF5B⁰⁹¹⁴³ (FlyBase, 1992-, ) P{PZ}l(3)09143⁰⁹¹⁴³ (BDGP Project Members, 1994-1999, FlyBase, 1992-) P{ry^+t7.2=PZ}l(3)09143⁰⁹¹⁴³ (FlyBase, 1992-) sprouty⁹¹⁴³-lacZ (Pallavi et al., 2006) sprouty-lacZ (Sen et al., 2004, Englund et al., 1999) Sprouty-lacZ (Sen et al., 2004) spry-lacZ (Iwanami et al., 2005) sty-lacZ (Sen et al., 2004)
Secondary FlyBase IDs
	FBti0000649 FBti0005595
References ( 22 )

Generate a list of	All references relating to this insertion ( 22 )

List References by type	Research paper ( 15 ) Personal communication to FlyBase ( 4 ) FlyBase analysis ( 3 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...

Dmel\P{PZ}eIF5B09143 Insertion

Dmel\P{PZ}eIF5B⁰⁹¹⁴³ Insertion