Dmel\P{lacW}L3659 Insertion

General Information
Symbol	Dmel\P{lacW}L3659	Species	D. melanogaster
Name		FlyBase ID	FBti0009930
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, eIF5B, l(3)L3659	Viability / fertility	viable
Causes allele(s)	Ecol\lacZ^L3659	Stock availability	2 publicly available
LINE ID	l(3)L3659
Genomic Location
Chromosomal location	3L ( 63E1 )	Sequence location	3L:3,460,609..3,460,609 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:3,460,609..3,460,609 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	63E1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	63D1-63D2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026254 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) eIF5B (Carrera et al., 2000) l(3)L3659 (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^L3659 (BDGP Project Members, 1994-1999)

Lethality References viable (Carrera et al., 2000)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)L3659 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10163 y¹ w¹¹¹⁸; P{lacW}L3659, l(3)L3659^L3659/TM3, Ser¹
Kyoto	111008 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}L3659 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3L:3444140-3444141 determined by FlyBase alignment of dbGSS accession AQ026254 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005) P{lacW}L3659 insertion maps 360bp upstream of the cIF2 initiation codon.Separable from: l(3)L3659^L3659.The P{lacW}L3659 insertion is separable from the lethality of the chromosome (which is caused by l(3)L3659^L3659). (Carrera et al., 2000)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}L3659 (FlyBase, 2005, FlyBase, 1992-) P{lacW}l(3)L3659^L3659 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Carrera et al., 2000, Molec. Cell 5(1): 181--187 VASA mediates translation through interaction with a Drosophila yIF2 homolog. [FBrf0125216] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]