A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}Hsc70-4L3929 Insertion

General Information
Symbol Dmel\P{lacW}Hsc70-4L3929 Species D. melanogaster
Name FlyBase ID FBti0009932
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) Ecol\lacZ, Hsc70-4 Viability / fertility
Causes allele(s) Ecol\lacZHsc70-4-L3929, Hsc70-4L3929 Stock availability 2 publicly available
LINE ID l(3)L3929
Genomic Location
Chromosomal location 3R ( 88E4 ) Sequence location 3R:11,068,448..11,068,448 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0128208 FBti0048088 FBti0077013 FBti0046276 FBti0066494 FBti0075843 FBti0004312 FBti0104265 FBti0011512 FBti0011513 FBti0100091 FBti0104765 FBti0016507 FBti0009932 FBti0058936 FBti0126101_2 FBti0126101_1 FBti0112794 FBti0056920 FBti0053412 FBti0143565 FBti0049748 FBti0058728 FBti0054838 FBti0025626 FBti0112800 FBti0067385 FBti0043026 FBti0076012 FBti0049511 FBti0011485 FBti0106110 FBti0106111 FBti0055480 FBti0111353 FBti0029539 FBti0109340 FBti0028521 FBti0027169 FBti0029028 FBti0030019 FBti0103964 FBti0056172 FBti0111083 FBti0100043 FBti0107411 FBti0103252 FBti0029023
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:11,068,448..11,068,448 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location
(computed by FlyBase)
88E4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
88E3-88E4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ026257
(FlyBase, 1992-,  
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Hsc70-4
(BDGP Project Members, 1994-1999, Kuronen et al., 2009)
hide Alleles and Phenotypes
Causes alleles
Ecol\lacZHsc70-4-L3929
(BDGP Project Members, 1994-1999)
Hsc70-4L3929
(BDGP Project Members, 1994-1999, Kuronen et al., 2009)
Lethality
References
lethal | recessive
(BDGP Project Members, 1994-1999)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
A Hsc70-4[L3929] mutant background enhances by more than 10-fold the level of vacuolar change in cathD[1] mutant retina.
(Kuronen et al., 2009)
18% of Hsc70-4L3929/Snr1E1 animals have an incomplete L5 wing vein.
(Marenda et al., 2004)
The CycEJP rough eye phenotype not suppressed by the addition of Hsc70-4L3929.
(Brumby et al., 2002)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(3)L3929
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 2 )
Bloomington
10286
y1 w1118; P{lacW}Hsc70-4L3929/TM3, Ser1
Kyoto
111040
yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}82B P{lacW}Hsc70-4L3929/TM6B, P{Car20y}TPN1, Tb1
hide Linkouts
hide Comments
Location 3R:11068448-11068449 confirmed by FlyBase alignment of dbGSS accession AQ026257 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
hsc70-4L3929
(Kuronen et al., 2009)
P{lacW}Hsc70-4L3929
(FlyBase, 2005, FlyBase, 1992-, )
P{lacW}l(3)L3929L3929
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Kuronen et al., 2009, Neurobiol. Disease 36(3): 488--493
Genetic modifiers of degeneration in the cathepsin D deficient Drosophila model for neuronal ceroid lipofuscinosis. [FBrf0209200]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Marenda et al., 2004, Dev. Biol. 267(2): 279--293
The Drosophila Brahma (SWI/SNF) chromatin remodeling complex exhibits cell-type specific activation and repression functions. [FBrf0174517]
Brumby et al., 2002, EMBO J. 21(13): 3377--3389
Drosophila cyclin E interacts with components of the Brahma complex. [FBrf0151282]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]