Dmel\P{lacW}Vps36^L5212 Insertion

General Information
Symbol	Dmel\P{lacW}Vps36^L5212	Species	D. melanogaster
Name		FlyBase ID	FBti0009942
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Ecol\lacZ, Vps36	Viability / fertility
Causes allele(s)	Ecol\lacZ^L5212, Vps36^L5212	Stock availability	1 publicly available
LINE ID	l(3)L5212
Genomic Location
Chromosomal location	3L ( 70B4 )	Sequence location	3L:13,506,150..13,506,150 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:13,506,150..13,506,150 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	70B4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	70B7-70C1 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026268 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Ecol\lacZ (BDGP Project Members, 1994-1999) Vps36 (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Ecol\lacZ^L5212 (BDGP Project Members, 1994-1999) Vps36^L5212 (BDGP Project Members, 1994-1999, Spradling et al., 1999, Irion and St Johnston, 2007)

Lethality References lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Sterility References female sterile \| germline clone \| recessive (Irion and St Johnston, 2007)
Phenotype Manifest In
	egg \| germline clone (Irion and St Johnston, 2007) eye \| somatic clone (Herz et al., 2009) eye-antennal disc \| somatic clone \| third instar larval stage (Herz et al., 2009)
Detailed Description
Statement Reference Heterozygosity for N[264-39] does not suppresses the rough-eye phenotype that occurs in animals with somatic clones of Vps36[L5212] generated in the eye-antennal disc. The eye-ablation phenotype in W[GMR.PG] animals is strongly suppressed in Vps36[L5212] somatic clones generated in the eye-antennal imaginal disc. (Herz et al., 2009) Vps36[L5212] somatic clones in eye-antennal imaginal discs contain enlarged, abnormal early endosomes. Vps36[L5212] somatic clones in eye-antennal imaginal discs show high levels of apoptosis compared to wildtype. Eye-antennal imaginal discs are comparable in size to wildtype, however in the adult a rough-eye phenotype is seen. When the majority of cells in the eye-antennal imaginal discs are mutant for Vps36[L5212], striking tissue overgrowth of the disc occurs and loose cellular architecture is observed. Cell proliferation does occur in Vps36[L5212] somatic clones in eye-antennal imaginal discs, and clones are unable to induce non-autonomous proliferation in surrounding wildtype tissue. (Herz et al., 2009) Females with homozygous germline clones do not lay eggs. (Irion and St Johnston, 2007)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)L5212 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	10176 y¹ w¹¹¹⁸; P{lacW}Vps36^L5212/TM3, Ser¹
Linkouts
Comments
	Location 3L:13486997-13486998 confirmed by FlyBase alignment of dbGSS accession AQ026268 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}l(3)L5212^L5212 (BDGP Project Members, 1994-1999, FlyBase, 1992-, FlyBase, 2005) P{lacW}Vps36^L5212
Secondary FlyBase IDs

References ( 10 )
Research paper	Herz et al., 2009, PLoS ONE 4(1): e4165 Common and distinct genetic properties of ESCRT-II components in Drosophila. [FBrf0206508] Irion and St Johnston, 2007, Nature 445(7127): 554--558 bicoid RNA localization requires specific binding of an endosomal sorting complex. [FBrf0193659] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Irion and St Johnston, 2007, Nature 445(7127): Supplementary methods. [FBrf0200158]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Vps36L5212 Insertion

Dmel\P{lacW}Vps36^L5212 Insertion