Dmel\P{lacW}Cdc27^L7123 Insertion

General Information
Symbol	Dmel\P{lacW}Cdc27^L7123	Species	D. melanogaster
Name		FlyBase ID	FBti0009953
Feature type	transposable_element_insertion_site
Description
Inserted element	P{lacW}	Expression data
Affected gene(s)	Cdc27, Ecol\lacZ	Viability / fertility
Causes allele(s)	Cdc27^L7123, Ecol\lacZ^Cdc27-L7123	Stock availability	2 publicly available
LINE ID	l(3)L7123
Genomic Location
Chromosomal location	3L ( 65E9 )	Sequence location	3L:7,242,314..7,242,314 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:7,242,314..7,242,314 [+] (BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)	65E9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	65F1-65F2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AQ026278 (FlyBase, 1992-,
Inserted Element
Construct	P{lacW}
Location-dependent role	lacZ enhancer trap (Bier et al., 1989)
Size	10.691Kb
Associated alleles	Ecol\lacZ^P\T.W w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Cdc27 (BDGP Project Members, 1994-1999, Huang and Raff, 2002) Ecol\lacZ (BDGP Project Members, 1994-1999)
Alleles and Phenotypes
Causes alleles	Cdc27^L7123 (BDGP Project Members, 1994-1999, Huang and Raff, 2002, Spradling et al., 1999, Deak, 1999.9.15, Deak et al., 2003) Ecol\lacZ^Cdc27-L7123 (BDGP Project Members, 1994-1999)

Lethality References lethal \| pharate adult stage \| recessive (Deak et al., 2003) lethal \| recessive (Spradling et al., 1999, BDGP Project Members, 1994-1999) semi-lethal \| female limited (Deak et al., 2003) semi-lethal \| recessive (Deak, 1999.9.15, Huang and Raff, 2002)
Sterility References female sterile (Deak, 1999.9.15) female sterile \| partially (Deak et al., 2003) sterile \| recessive (Huang and Raff, 2002)
Phenotype Manifest In
	aster & neuroblast (Deak et al., 2003) chaeta (Huang and Raff, 2002) chromatin & neuroblast (Deak et al., 2003) chromosome & neuroblast (Deak et al., 2003) eye (Huang and Raff, 2002) mitotic metaphase & neuroblast (Deak et al., 2003) spindle & neuroblast (Deak et al., 2003)
Detailed Description
Statement Reference The slight increase in lymph gland size seen in Zfrp8[SM206]/+ larvae is dominantly enhanced by Cdc27[L7123]. (Minakhina et al., 2007) Examination of the larval stage central nervous system of Bub1^unspecified Cdc27^L7123 double mutant shows cells arrested at metaphase at a comparable frequency to the Cdc27^L7123 mutant alone. Moreover, the high frequency of anaphases seen in the Bub1^unspecified mutant as a result of the malfunctioning checkpoint is replaced in the double mutant by an elevated frequency of metaphase-like figures typical of Cdc27^L7123. The appearance of mitotic figures in squashed preparations of Bub1^unspecified Cdc27^L7123 is indistinguishable from those seen in Cdc27^L7123 alone. (Deak et al., 2003) In Cdc27^L7123 mutants, there is a high proportion of cells in a metaphase-like state (a ratio of 8.7 metaphase:anaphase compared to 2.8 in wild-type). Along with the low proportion of cells in anaphase, this suggests that Cdc27^L7123 cells arrest or delay in metaphase. The extent of chromosome condensation observed indicates that cells have been delayed in metaphase for a period of time. Cdc27^L7123 anaphase cells are frequently disorganised, with chromosomes lagging along the spindle. A low frequency (~3% of mitoses) of polyploid cells are also found in Cdc27^L7123 cells. In Cdc27^L7123 larval stage brains the sister chromosomes of at least one chromosome are found to be separated in 20% of all mitotic figures. 19% of Cdc27^L7123 mitotic cells are found to contain a 4N complement of chromosomes in which there are four sites of hybridization indicating that the sister chromosomes are separated. This is compared to a frequency of 0.4% in wild-type metaphase cells. In mitotic Cdc27^L7123 cells, the centromeric regions of the two pairs of major autosomes are present as a series of four separate pairs of dots, indicative of chromosome separation. Approximately 3.9% of Cdc27^L7123 cells contain a replicated tetraploid set of chromosomes. Chromatin in Cdc27^L7123 mitotic cells is distributed in a broad band. Cdc27^L7123 cells exhibit overcondensed chromosomes. Up to 16 separated kinetochores of sister chromosomes are observed in Cdc27^L7123 cells, similar to the phenotype observed in polo hypomorphs. (Deak et al., 2003) Examination of the larval stage central nervous system of BubR1^unspecified Cdc27^L7123 double mutant shows cells arrested at metaphase at a comparable frequency to the Cdc27^L7123 mutant alone. Moreover, the high frequency of anaphases seen in the BubR1^unspecified mutant as a result of the malfunctioning checkpoint is replaced in the double mutant by an elevated frequency of metaphase-like figures typical of Cdc27^L7123. The appearance of mitotic figures in squashed preparations of BubR1^unspecified Cdc27^L7123 is indistinguishable from those seen in Cdc27^L7123 alone. (Deak et al., 2003) Homozygous flies occasionally survive to adulthood. These escapers have many eye and bristle defects, are invariably sterile and only live for a few days. (Huang and Raff, 2002)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	l(3)L7123 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	10168 y¹ w¹¹¹⁸; P{lacW}Cdc27^L7123/TM3, Ser¹
Kyoto	111012 y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Cdc27^L7123 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹
Linkouts
Comments
	Location 3L:7223161-7223162 confirmed by FlyBase alignment of dbGSS accession AQ026278 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. (FlyBase, 2005)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{lacW}Cdc27^L7123 (FlyBase, 2005, FlyBase, 1992-) P{lacW}l(3)L7123^L7123 (BDGP Project Members, 1994-1999, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 13 )
Research paper	Minakhina et al., 2007, Development 134(13): 2387--2396 Zfrp8, the Drosophila ortholog of PDCD2, functions in lymph gland development and controls cell proliferation. [FBrf0201958] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Deak et al., 2003, J. Cell Sci. 116(20): 4147--4158 Mutations in makos, a Drosophila gene encoding the Cdc27 subunit of the anaphase promoting complex, enhance centrosomal defects in polo and are suppressed by mutations in twins/aar, which encodes a regulatory subunit of PP2A. [FBrf0162197] Huang and Raff, 2002, J. Cell Sci. 115(14): 2847--2856 The dynamic localisation of the Drosophila APC/C: evidence for the existence of multiple complexes that perform distinct functions and are differentially localised. [FBrf0149067] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Bier et al., 1989, Genes Dev. 3: 1273--1287 Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Supplementary material	Parks et al., 2004, Nature Genetics 36(3): Supplementary Table 1: Deletions generated at Bloomington between trans-heterozygous P element insertions. [FBrf0179070]
Personal communication to FlyBase	Andrade and Cook, 2003.4.2, Isolation and characterization of Df(3L)BSC33. Isolation and characterization of Df(3L)BSC33. [FBrf0157305] Deak, 1999.9.15, mks corresponds to cdc27. mks corresponds to cdc27. [FBrf0111595]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{lacW}Cdc27L7123 Insertion

Dmel\P{lacW}Cdc27^L7123 Insertion