A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{lacW}slik04807b Insertion

General Information
Symbol Dmel\P{lacW}slik04807b Species D. melanogaster
Name FlyBase ID FBti0010019
Feature type transposable_element_insertion_site
Description
Inserted element P{lacW} Expression data
Affected gene(s) sli Viability / fertility
Causes allele(s) slik04807b Stock availability 1 publicly available
LINE ID l(2)k04807
Genomic Location
Chromosomal location 2R ( 52C9-52D2 ) Sequence location
Member of Large Scale Dataset(s)
Dataset

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w[+mC] mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
(Spradling et al., 1999, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location
(computed by FlyBase)
52C9-52D2 ( near gene of known cytology )
Cytological location
(reported)
52D1-52D2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
hide Inserted Element
Construct P{lacW}
Location-dependent
role
lacZ enhancer trap
(Bier et al., 1989)
Size 10.691Kb
Associated alleles
Ecol\lacZP\T.W
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
sli
(Pappu et al., 2011, Tayler et al., 2004, Fan et al., 2005)
hide Alleles and Phenotypes
Causes alleles
slik04807b
(Tayler et al., 2004, Fan et al., 2005, Pappu et al., 2011)
Lethality
References
viable
(Tayler et al., 2004)
Sterility
References
hide Phenotype Manifest In
chiasm
(Pappu et al., 2011)
eye photoreceptor cell
(Pappu et al., 2011)
eye photoreceptor cell & axon
(Tayler et al., 2004)
lamina anlage
(Tayler et al., 2004)
lamina anlage glial cell
(Tayler et al., 2004)
lamina neuropil
(Tayler et al., 2004)
photoreceptor cell R1 & axon
(Tayler et al., 2001)
photoreceptor cell R2 & axon
(Tayler et al., 2001, Tayler et al., 2004)
photoreceptor cell R3 & axon
(Tayler et al., 2001, Tayler et al., 2004)
photoreceptor cell R4 & axon
(Tayler et al., 2001, Tayler et al., 2004)
photoreceptor cell R5 & axon
(Tayler et al., 2001, Tayler et al., 2004)
photoreceptor cell R6 & axon
(Tayler et al., 2001)
hide Detailed Description
Statement
Reference
Photoreceptor cell axons do not form an optic chiasm in sli[k04807b] mutants.
(Pappu et al., 2011)
In egh7/Y; slik04807b/+ mutants, the R-cell axonal projection pattern is defective like in egh7 single mutants. egh7/+; slik04807b/+ mutants appear wild type.
(Fan et al., 2005)
There are gaps in the lamina neuropil in the visual system of homozygous third instar larvae and increased numbers of photoreceptor axons enter the medulla; many R2-R5 axons pass through the lamina and enter the medulla, in contrast to wild type where they stop in the lamina neuropil. slik04807b/sli05248, slik04807b/sliE-158 and slik04807b/sli2 third instar larvae show defects in photoreceptor axon targeting, with gaps in the lamina and increased numbers of axons entering the medulla. slik04807b/sli2 animals show disruption of the lamina glia layer; clumps of glia and gaps in the glial layers are seen. The regions of photoreceptor axon mistargeting correlate with areas of lamin glial disruption. Many distal cell neurons enter the base of the lamina in slik04807b/sli2 third instar larvae and some distal cell neurons invade the lamina neuropil, disrupting photoreceptor innervation.
(Tayler et al., 2004)
Mutant strongly disrupts the target layer selections of R1-R6 photoreceptor axons.
(Tayler et al., 2001)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
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FlyView (LinkOut)
hide Data on Genetic Line
Line ID
l(2)k04807
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
t
(Gene Disruption Project members, 2001-)
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
9284
w*; P{lacW}slik04807b, P{GMR-GFP.P}2/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7
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hide Comments
NOTE: derived from line carrying multiple insertions.
(BDGP Project Members, 1994-1999)
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
(FlyBase, 1992-)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
l(2)k04807
(FlyBase, 1992-)
P{lacW}k04807b
(FlyBase, 1992-)
P{lacW}slik04807b
(FlyBase, 1992-, )
slitdui
(Pappu et al., 2011)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Pappu et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(18): 7571--7576
Robo-3-mediated repulsive interactions guide R8 axons during Drosophila visual system development. [FBrf0213605]
Fan et al., 2005, Dev. Biol. 287(1): 61--73
The egghead gene is required for compartmentalization in Drosophila optic lobe development. [FBrf0190118]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Tayler et al., 2004, Development 131(23): 5935--5945
Compartmentalization of visual centers in the Drosophila brain requires Slit and Robo proteins. [FBrf0180181]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Bier et al., 1989, Genes Dev. 3: 1273--1287
Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. [FBrf0049800]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
Abstract
Tayler et al., 2001, Bellen, Taylor, 2001: 87
A tissue-specific allele of Slit uncovers its role in visual system axon targeting. [FBrf0144537]
FlyBase analysis
FlyBase Curators, 2013, Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}.
Members of BDGP/GDP insertion collections: P{hsneo}, P{PZ}, P{lacW}. [FBrf0220600]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]