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General Information
Symbol
Dmel\P{PZ}wek05271a
Species
D. melanogaster
Name
FlyBase ID
FBti0010222
Description
Inserted Element
Uses
lacZ reporter
Affected gene(s)
Viability / fertility
fertile, viable
Associated allele(s)
Stock availability
LINE ID
l(2)05271
Genomic Location
Chromosomal Location
2L ( 35D4 )
Sequence Location
2L:15,768,312..15,768,312
Target / Docking site
Member of Large Scale Dataset(s)
Description
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:15,768,312..15,768,312
Orientation
Cytological location (computed by FlyBase)
35D4 (inferred by FlyBase from sequence location)
Cytological location (reported)
35D1--35D2 (in situ hybridization reported)
35D1-35D2 (in situ hybridization reported)
Insertion into Natural transposon
Comments concerning location
The insertion site was reported to be nucleotide 6 of wek. The start of GB:AY051673 was used as the start of transcription the mapping.
Sequence Data
Flanking sequence
Inserted Element
Construct
Location-dependent role
lacZ enhancer trap
Size (Kb)
14.545
Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference
Enhancer trap expression is induced by ecdysone in third instar leg imaginal discs.
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments
The orientation of the insertion was not reported.
Insertion separable from lethal originally isolated on the same chromosome.
Insertion separable from lethal originally isolated on the same chromosome; see report for l(2)05271b05271b.
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P{PZ}l(2)0527105271
P{PZ}l(2)35Ea05271
P{PZ}l(2)35Ea05271a
P{PZ}wek05271a
Name Synonyms
Secondary FlyBase IDs
    References (13)