FlyBase Insertion Report: Dmel\P{lacW}Btk[k00206]

General Information

Symbol

Dmel\P{lacW}Btk^k00206

Species

D. melanogaster

Name

FlyBase ID

FBti0010229

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{lacW}

LINE ID

l(2)k00206

Affected gene(s)

Btk, Ecol\lacZ

Viability / fertility

Associated allele(s)

Btk^k00206, Ecol\lacZ^Btk-k00206

Stock availability

2 publicly available

Genomic Location

Chromosomal Location

2L ( 29A1 )

Sequence Location

2L:8,277,187..8,277,187 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:8,277,187..8,277,187 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

29A1 (inferred by FlyBase from sequence location)

Cytological location (reported)

29A1-29A2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2L:8277178..8277186 .

Sequence Data

Flanking sequence

AQ034168

(FlyBase, 1992-)

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Btk

(Öztürk-Çolak et al., 2018, Jagut et al., 2013, Chandrasekaran and Beckendorf, 2005, Djagaeva, 2005, Djagaeva et al., 2005, Read et al., 2004, Schnorr et al., 2001, Spradling et al., 1999, Roulier et al., 1998, BDGP Project Members, 1994-1999)

Ecol\lacZ

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Btk^k00206

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^Btk-k00206

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)k00206

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (2)

Bloomington

10469

y¹ w^67c23; P{lacW}Btk^k00206/CyO

Kyoto

111118

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Btk^k00206 P{neoFRT}40A/CyO y⁺

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:8277187-8277188 confirmed by FlyBase alignment of dbGSS accession AQ034168 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

NOTE: derived from line carrying multiple insertions.

(BDGP Project Members, 1994-1999)

8 bp insertion associated host repeat

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Btk^k00206

(Fernández et al., 2014)

P{lacW]k00206

(FlyBase, 1992-)

P{lacW}Btk29A^k00206

(FlyBase, 2005, FlyBase, 1992-)

P{lacW}Btk^k00206

P{lacW}k00206

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (24)

Report Sections

Open Close