Dmel\P{EP}aop^EP598 Insertion

General Information
Symbol	Dmel\P{EP}aop^EP598	Species	D. melanogaster
Name		FlyBase ID	FBti0010416
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	aop	Viability / fertility
Causes allele(s)	aop^EP598	Stock availability	none publicly available
LINE ID	EP(2)0598
Genomic Location
Chromosomal location	2L ( 22D1 )	Sequence location	2L:2,178,792..2,178,792 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:2,178,792..2,178,792 [-] (FlyBase, 2005) 2L:2,178,785..2,178,792 [-] (Abdelilah-Seyfried et al., 2000)
Orientation
Cytological location (computed by FlyBase)	22D1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	22D1-22D1 (reported as inferred from sequence location) (BDGP Project Members, 2000-) 22D1-22D2 (in situ hybridization reported) (BDGP Project Members, 1994-1999)
Comments concerning location
Sequence Data
Flanking sequence	AZ300999 (FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	aop (Rorth et al., 1998, Weber et al., 2008, Kim et al., 2010, Abdelilah-Seyfried et al., 2000)
Alleles and Phenotypes
Causes alleles	aop^EP598 (van Swinderen and Greenspan, 2005, Rorth et al., 1998, Warrick et al., 1999, Singh et al., 2005, Weber et al., 2008, Tseng and Hariharan, 2002, Abdelilah-Seyfried et al., 2000, Gene Disruption Project members, 2001-, Kim et al., 2010)

Lethality References lethal (Tseng and Hariharan, 2002)
Sterility References
Phenotype Manifest In
	adult abdomen & trichogen cell (Abdelilah-Seyfried et al., 2000) scutellar bristle (Abdelilah-Seyfried et al., 2000) scutellar bristle \| ectopic (Abdelilah-Seyfried et al., 2000) tormogen cell (Abdelilah-Seyfried et al., 2000) tormogen cell \| ectopic (Abdelilah-Seyfried et al., 2000) trichogen cell \| ectopic (Abdelilah-Seyfried et al., 2000) wing (Rorth et al., 1998) wing vein (Rorth et al., 1998, Tseng and Hariharan, 2002)
Detailed Description
Statement Reference aop[EP598] suppresses the rough eye phenotype seen when Dcp-1[Scer\UAS.cKa] (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4[GMR.PF]. (Kim et al., 2010) Co-expression of aop[EP598] partially suppresses the symmetrical ommatidia phenotype caused by expression of pnt[P2.Scer\UAS] under the control of Scer\GAL4[hs.2sev]. (Weber et al., 2008) Flies expressing aop^EP598 under the control of Scer\GAL4^hs.2sev have wild-type eyes. Flies expressing aop^EP598 under the control of Scer\GAL4^en-e16E have incomplete wing venation. (Tseng and Hariharan, 2002) Expression of aop^EP598 under the control of Scer\GAL4^sca-537.4 results in too many or too few scutellar macrochaetae. Sensory organs with no socket and one shaft, one socket and two shafts and two sockets and one shaft are seen. On the abdomen short shaft morphology is seen. (Abdelilah-Seyfried et al., 2000) Expression of aop^EP598 under the control of Scer\GAL4^bi-omb-Gal4 produces wings of reduced size with mild vein pattern defects. (Rorth et al., 1998)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)0598 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 2L:2178791-2178792 determined by FlyBase alignment of dbGSS accession AZ300999 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005) 8bp insertion associated host repeat. The P{EP} insertion site was reported at -98bp relative to the transcription start. The orientation of the insertion was not reported. (Abdelilah-Seyfried et al., 2000) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP598 (van Swinderen and Greenspan, 2005) EP(2)0598 (Kim et al., 2010) P{EP}aop^EP598 (Rorth et al., 1998, FlyBase, 2005, FlyBase, 1992-, Abdelilah-Seyfried et al., 2000, ) P{EP}EP0598 (FlyBase, 1992-) P{EP}EP598 (FlyBase, 1992-) UAS-yan (Warrick et al., 1999) UAS-yan^EP<up>598</up> (Weber et al., 2008)
Secondary FlyBase IDs
	FBti0010718
References ( 16 )
Research paper	Kim et al., 2010, BMC Cell Biol. 11: 9 A genetic screen for modifiers of Drosophila caspase Dcp-1 reveals caspase involvement in autophagy and novel caspase-related genes. [FBrf0209948] Weber et al., 2008, Dev. Biol. 316(1): 110--123 Combinatorial signaling by the Frizzled/PCP and Egfr pathways during planar cell polarity establishment in the Drosophila eye. [FBrf0204109] Singh et al., 2005, Genetics 171(1): 169--183 Genetic interaction of Lobe with its modifiers in dorsoventral patterning and growth of the Drosophila eye. [FBrf0187660] van Swinderen and Greenspan, 2005, Genetics 169(4): 2151--2163 Flexibility in a gene network affecting a simple behavior in Drosophila melanogaster. [FBrf0188513] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Tseng and Hariharan, 2002, Genetics 162(1): 229--243 An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. [FBrf0152035] Abdelilah-Seyfried et al., 2000, Genetics 155(2): 733--752 A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ. [FBrf0128367] Warrick et al., 1999, Nat. Genet. 23(4): 425--428 Suppression of polyglutamine-mediated neurodegeneration in Drosophila by the molecular chaperone HSP70. [FBrf0123236] Rorth et al., 1998, Development 125(6): 1049--1057 Systematic gain-of-function genetics in Drosophila. [FBrf0101995] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}aopEP598 Insertion

Dmel\P{EP}aop^EP598 Insertion