Dmel\P{PZ}amn^chpd Insertion

General Information
Symbol	Dmel\P{PZ}amn^chpd	Species	D. melanogaster
Name		FlyBase ID	FBti0010693
Feature type	transposable_element_insertion_site
Description
Inserted element	P{PZ}	Expression data	lacZ reporter
Affected gene(s)	amn, Ecol\lacZ	Viability / fertility
Causes allele(s)	amn^chpd, Ecol\lacZ^amn-chpd	Stock availability	1 publicly available
LINE ID
Genomic Location
Chromosomal location	X ( 18F4-19A2 )	Sequence location
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)	18F4-19A2 ( near gene of known cytology )
Cytological location (reported)
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct	P{PZ}
Location-dependent role	lacZ enhancer trap (Mlodzik and Hiromi, 1992)
Size	14.545Kb
Associated alleles	Ecol\lacZ^P\T.PZ l(3)87Df^7.2 ry^+t7.2
Molecular map
Affected Gene(s)
Insertion may affect gene	amn (Moore et al., 1998) Ecol\lacZ (Waddell et al., 2000)
Alleles and Phenotypes
Causes alleles	amn^chpd (Moore et al., 1998, Wolf et al., 2002) Ecol\lacZ^amn-chpd (Waddell et al., 2000)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference amn[chpd] mutants do not exhibit any behavioral memory at 24 hours and only a small amount is present at 9 hours after conditioning. Unlike wild-type, there is no significant increase in odor-evoked calcium influx into the α branch of the mushroom body neurons in amn[chpd] mutants at either 9 or 24 hours after conditioning. (Yu et al., 2006) amn^chpd flies have a defective olfactory memory. These flies fail to produce the increased calcium influx seen in the dorsal paired medial neurons of wild-type flies at 30 minutes after a conditioning of 3s exposure to odour, followed by a 60s odour stimulus applied simultaneously with 12 electric-shock pulses. (Yu et al., 2005) amn^chpd mutant flies show similar response profiles to wild-type flies in response to ethanol: flies have an initial startle response, a brief moment of quiescence followed by a sustained period of hyperactivity and a reduction in locomotor speed leading to eventual immobility. However, the amn^chpd mutants have a more intense hyperactive phase and reach maximal hyperactivity more quickly, leading to a faster onset of immobility. During the hyperactive phase, amn^chpd mutants initiate activity bouts less often than wild-type flies, but stay active for longer and spend more time moving fast. As these mutants begin to sedate, their bout frequency actually increases but their bout length is shorter. (Wolf et al., 2002) amn^chpd rut²⁰⁸⁰ double mutants do not show increased sensitivity to ethanol in an inebriometer assay, although each single mutant does show increased sensitivity to ethanol in an inebriometer assay. (Moore et al., 1998) Hemizygous males and homozygous females show increased sensitivity to ethanol; they elute from an inebriometer with a mean elution time (MET) of approximately 15 minutes compared with an MET of 20 minutes for wild-type controls. amn^chpd flies show normal geotaxis and locomotor activity. Ethanol absorption and metabolism is normal in these flies. The ethanol-sensitive phenotype is reversed by treatment of the flies with forskolin (an adenylate cyclase activator) for 2 hours. A 4 hour treatment with forskolin further reduces ethanol sensitivity of amn^chpd flies compared with control flies. Treatment of amn^chpd flies with a cAMP analog that activates PKA also reverses the ethanol-sensitive phenotype. Treatment of amn^chpd flies with an antagonist of cAMP that inhibits PKA partially reverses the ethanol-sensitive phenotype. (Moore et al., 1998) The ethanol sensitive phenotype is rescued by amn^hs.PM expressed using heat shock. Full rescue of the ethanol-sensitive phenotype is seen in animals given a daily heat shock throughout development, and is still seen 48 hours after the last heat shock, but the phenotype has reverted to ethanol-sensitivity 72 hours after the last heat shock. Three heat shocks at 24 hour intervals in adult flies is also sufficient to fully rescue the ethanol-sensitive phenotype. (Moore et al., 1998)
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference adult stage mushroom body dorsal paired medial cell (Waddell et al., 2000)
Additional Information
Statement Reference Expression is based on lacZ activity of a P-element inserted in the amn locus and reflects amn. (Waddell et al., 2000)

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	26499 P{PZ}amn^chpd; P{neoFRT.clipped}40A P{FRT(w^hs).clipped}G13; ry⁵⁰⁶
Linkouts
Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PZ}amn^chpd (Moore et al., 1998, FlyBase, 1992-) unnamed (Waddell et al., 2000)
Secondary FlyBase IDs

References ( 7 )
Research paper	Yu et al., 2006, Neuron 52(5): 845--855 Drosophila alpha/beta mushroom body neurons form a branch-specific, long-term cellular memory trace after spaced olfactory conditioning. [FBrf0194016] Yu et al., 2005, Cell 123(5): 945--957 Drosophila DPM neurons form a delayed and branch-specific memory trace after olfactory classical conditioning. [FBrf0189930] Wolf et al., 2002, J. Neurosci. 22(24): 11035--11044 High-resolution analysis of ethanol-induced locomotor stimulation in Drosophila. [FBrf0155893] Waddell et al., 2000, Cell 103(5): 805--813 The amnesiac gene product is expressed in two neurons in the Drosophila brain that are critical for memory. [FBrf0132261] Moore et al., 1998, Cell 93(6): 997--1007 Ethanol intoxication in Drosophila: Genetic and pharmacological evidence for regulation by the cAMP signaling pathway. [FBrf0102808] Mlodzik and Hiromi, 1992, Conn, 1992: 397--414 Enhancer trap method in Drosophila: its application to neurobiology. [FBrf0066714]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]

Dmel\P{PZ}amnchpd Insertion

Dmel\P{PZ}amn^chpd Insertion