Dmel\P{EP}Traf4^EP578 Insertion

General Information
Symbol	Dmel\P{EP}Traf4^EP578	Species	D. melanogaster
Name		FlyBase ID	FBti0010715
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Traf4	Viability / fertility
Causes allele(s)	Traf4^EP578	Stock availability	1 publicly available
LINE ID	EP(2)0578
Genomic Location
Chromosomal location	2L ( 24E1 )	Sequence location	2L:4,371,955..4,371,955 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:4,371,955..4,371,955 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	24E1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	24E3-24E4 (in situ hybridization reported) (BDGP Project Members, 1994-1999) 24F1-24F1 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ254802 (FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Traf4 (BDGP Project Members, 2000-, Tiwari and Roy, 2009, Wang et al., 2006, Mallik and Lakhotia, 2009, Vicente-Crespo et al., 2008, Mathew et al., 2009)
Alleles and Phenotypes
Causes alleles	Traf4^EP578 (Tseng and Hariharan, 2002, Cha et al., 2005, Kuranaga et al., 2002, Cha et al., 2003, Tiwari and Roy, 2009, Wang et al., 2006, Mallik and Lakhotia, 2009, Vicente-Crespo et al., 2008, Mathew et al., 2009)

Lethality References
Sterility References
Phenotype Manifest In
	eye (Tseng and Hariharan, 2002, Cha et al., 2003, Tiwari and Roy, 2009, Mallik and Lakhotia, 2009) eye disc (Cha et al., 2003) eye photoreceptor cell (Cha et al., 2003) ommatidium (Cha et al., 2003)
Detailed Description
Statement Reference Homozygous flies are resistant to V. cholerae infection compared to controls. (Berkey et al., 2009) Scer\GAL4[GMR.PF]-mediated expression of Traf4[EP578] results in rough eyes with disorganized arrays of ommatidia. (Mallik and Lakhotia, 2009) Homozygous Traf4[EP578] mutant embryos appear normal. (Mathew et al., 2009) Expression of Traf4[EP578] under the control of Scer\GAL4[GMR.PF] results in a rough eye phenotype. (Tiwari and Roy, 2009) The reduced eye phenotype caused by expression of Rab11[N124I.Scer\UAS] under the control of Scer\GAL4[GMR.PF] is enhanced by co-expression of Traf4[EP578]. (Tiwari and Roy, 2009) A Traf4[EP578] mutant background suppresses the moderately rough eye phenotype observed upon expression of mbl[C.Scer\UAS] under the control of Scer\GAL4[hs.2sev]. (Vicente-Crespo et al., 2008) The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) is suppressed by co-expression of Traf1^EP578. (Cha et al., 2005) Overexpression of Traf1^EP578, under the regulation of Scer\GAL4^ap-md544 has no effect on thorax closure. (Cha et al., 2005) Expression of Traf1^EP578 under the control of Scer\GAL4^GMR.PF leads to a rough eye phenotype. Eyes have a reduced number of ommatidia, abnormally-shaped photoreceptor cells and an overall reduction in the size of the compound eye. This phenotype is more severe when two copies of Traf1^EP578 are expressed. The eye imaginal discs from transgenic flies overexpressing Traf1^EP578 under the control of Scer\GAL4^GMR.PF contain a highly increased number of apoptotic cells in the region posterior to the morphogenetic furrow in a gene dosage-dependent manner. (Cha et al., 2003) Coexpression of bsk^Scer\UAS.cBa with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, leads to an increase in the disturbance of the ommatidial array in the compound eye in comparison to the eye phenotype resulting from one copy overexpression of Traf1^EP578. When hep^Scer\UAS.cBa is coexpressed with one copy of Traf1^EP578, driven by Scer\GAL4^GMR.PF, the number of ommatidia and the size of the compound eye are reduced to similar levels seen when two copies of Traf1^EP578 are expressed. Expression of Traf1^EP578, under the control of Scer\GAL4^GMR.PF, in a hep¹/Y background, results in the recovery of an eye of wild-type appearance. The number of apoptotic cells in eye dsics of this genotype are decreased compared to Scer\GAL4^GMR.PF>Traf1^EP578 discs. Coexpression of Tak1^Scer\UAS.cTa, with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, an increased eye roughness and reduced eye size compared to expression of just Traf1^EP578. Scer\GAL4^GMR.PF>Traf1^EP578 expression in a Tak1¹ background block the rough-eye phenotype caused by Traf1^EP578 overexpression. Coexpression of Traf2^EP1516 with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, does not alter the rough-eye phenotype caused by sole overexpression of Traf1^EP578. (Cha et al., 2003) The rpr^GMR.PW eye phenotypes are significantly suppressed by heterozygosity for Traf1^EP578. (Kuranaga et al., 2002) Co-expression of CycE^Scer\UAS.cLa suppresses the small eye phenotype caused by expression of Traf1^EP578 under the control of Scer\GAL4^ey.PH. (Tseng and Hariharan, 2002) Expression of Traf1^EP578 under the control of Scer\GAL4^ey.PH results in eyes that are somewhat reduced in size. Flies expressing Traf1^EP578 under the control of Scer\GAL4^hs.2sev have wild-type eyes. Flies expressing Traf1^EP578 under the control of Scer\GAL4^en-e16E have wild-type wings. (Tseng and Hariharan, 2002)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)0578 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17285 w¹¹¹⁸; P{EP}Traf4^EP578
Linkouts
Comments
	Location 2L:4371955-4371956 confirmed by FlyBase alignment of dbGSS accession AQ254802 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP0578 (Wang et al., 2006, Mallik and Lakhotia, 2009) EP(2)0578 (Cha et al., 2005, FlyBase, 1992-, Cha et al., 2003, Tiwari and Roy, 2009) EP(2)578 (Kuranaga et al., 2002) P{EP}EP0578 (FlyBase, 1992-) P{EP}EP578 (FlyBase, 1992-) P{EP}Traf1^EP578 (FlyBase, 1992-, FlyBase, 2005) P{EP}Traf4^EP578 Traf1^EP578 (Vicente-Crespo et al., 2008) Traf4^EP578 (Mathew et al., 2009) UAS-DTRAF1 (Mallik and Lakhotia, 2009)
Secondary FlyBase IDs

References ( 18 )
Research paper	Berkey et al., 2009, Cell. Microbiol. 11(3): 461--474 Genetic analysis of Drosophila melanogaster susceptibility to intestinal Vibrio cholerae infection. [FBrf0207589] Mallik and Lakhotia, 2009, Genetics 183(3): 831--852 The developmentally active and stress-inducible noncoding hsromega gene is a novel regulator of apoptosis in Drosophila. [FBrf0209343] Mathew et al., 2009, PLoS ONE 4(10): e7437 A small genomic region containing several loci required for gastrulation in Drosophila. [FBrf0208913] Tiwari and Roy, 2009, Europ. J. Cell Biol. 88(8): 445--460 Mutation in Rab11 results in abnormal organization of ommatidial cells and activation of JNK signaling in the Drosophila eye. [FBrf0208143] Vicente-Crespo et al., 2008, PLoS ONE 3(2): e1613 Drosophila muscleblind is involved in troponin T alternative splicing and apoptosis. [FBrf0210274] Wang et al., 2006, EMBO J. 25(24): 5783--5793 Drosophila homologs of mammalian TNF/TNFR-related molecules regulate segregation of Miranda/Prospero in neuroblasts. [FBrf0192517] Cha et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(29): 10345--10350 Parkin negatively regulates JNK pathway in the dopaminergic neurons of Drosophila. [FBrf0188253] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Cha et al., 2003, Mol. Cell. Biol. 23(22): 7982--7991 Discrete functions of TRAF1 and TRAF2 in Drosophila melanogaster mediated by c-Jun N-terminal kinase and NF-kappaB-dependent signaling pathways. [FBrf0167884] Kuranaga et al., 2002, Nat. Cell Biol. 4(9): 705--710 Reaper-mediated inhibition of DIAP1-induced DTRAF1 degradation results in activation of JNK in Drosophila. [FBrf0152246] Tseng and Hariharan, 2002, Genetics 162(1): 229--243 An overexpression screen in Drosophila for genes that restrict growth or cell-cycle progression in the developing eye. [FBrf0152035] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}Traf4EP578 Insertion

Dmel\P{EP}Traf4^EP578 Insertion