Dmel\P{EP}garz^EP2028 Insertion

General Information
Symbol	Dmel\P{EP}garz^EP2028	Species	D. melanogaster
Name		FlyBase ID	FBti0010785
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	CG8841, garz	Viability / fertility
Causes allele(s)	garz^EP2028	Stock availability	2 publicly available
LINE ID	EP(2)2028
Genomic Location
Chromosomal location	2R ( 48F8 )	Sequence location	2R:8,213,881..8,213,881 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:8,213,881..8,213,881 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	48F8 ( inferred by FlyBase from sequence location )
Cytological location (reported)	48F7-48F8 (in situ hybridization reported) (BDGP Project Members, 1994-1999) 48F10-48F10 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ074040 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	CG8841 (BDGP Project Members, 2000-) garz (Wang et al., 2012, Kraut et al., 2001, Shulman and Feany, 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Armbruster and Luschnig, 2012)
Alleles and Phenotypes
Causes alleles	garz^EP2028 (Kraut et al., 2001, Shulman and Feany, 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Wang et al., 2012, Armbruster and Luschnig, 2012)

Lethality References lethal (Wang et al., 2012) lethal \| first instar larval stage (Wang et al., 2012)
Sterility References
Phenotype Manifest In
	anterior fascicle & synapse (Kraut et al., 2001) anterior fascicle & synapse \| supernumerary (Kraut et al., 2001) eye (Shulman and Feany, 2003) eye photoreceptor cell (Raghu et al., 2009) intersegmental nerve (Kraut et al., 2001, Kraut et al., 2001)
Detailed Description
Statement Reference Lethal phase analysis of garz[EP2028] homozygous mutants reveals early first instar larval lethality with a very low number of individuals surviving until the end of larval development. (Wang et al., 2012) Flies expressing garz[EP2028] under the control of Scer\GAL4[GMR.PF] in the dark show normal rhabdomere development. Flies expressing garz[EP2028] under the control of Scer\GAL4[GMR.PF] and grown in bright light show some defects in rhabdomere structure and mild accumulation of endomembranes in the cell body of the photoreceptors. (Raghu et al., 2009) Co-expression of garz[EP2028] strongly enhances the retina phenotype caused by expression of Pld[Scer\UAS.cRa] under the control of Scer\GAL4[GMR.PF]; only one or two poorly developed rhabdomeres are seen. Expression of garz[EP2028] under the control of Scer\GAL4[GMR.PF] in a CdsA[1] background under bright light illumination results in significantly worse defects in rhabdomere size and structure compared to CdsA[1] single mutants of flies expressing garz[EP2028] under the control of Scer\GAL4[GMR.PF] in a wild-type background. There is no change in the accumulation of membranes in the cell body of the photoreceptors in the double mutant flies. (Raghu et al., 2009) The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by garz^EP2028. (Shulman and Feany, 2003) Expression of garz^EP2028 under the control of Scer\GAL4^GMR.PF results in a very mild rough eye phenotype. (Shulman and Feany, 2003) In the ventral muscle region of mutant larvae expressing garz^EP2028 under the control of Scer\GAL4^elav-C155 ISN pathfinding defects can be seen. ISNb leaves the ISN at an abnormal point. It generates an abnormal axonal branch that splits into synaptic branches on incorrect muscles. (Kraut et al., 2001) Mutant larvae expressing garz^EP2028 under the control of Scer\GAL4^elav-C155 have excessive or ectopic synapses. Mutant larvae expressing garz^EP2028 under the control of Scer\GAL4^elav-C155 have reduced or abnormal synapses. (Kraut et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2028 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	17017 w¹¹¹⁸; P{EP}garz^EP2028/CyO
Kyoto	114444 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}42D P{EP}garz^EP2028 /CyO y⁺
Linkouts
Comments
	Location 2R:7842283-7842284 confirmed by FlyBase alignment of dbGSS accession AQ074040 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(2)2028 (Shulman and Feany, 2003, FlyBase, 1992-, Wang et al., 2012) EP(2) 2028 (Kraut et al., 2001, Kraut et al., 2001) P{EP}CG8487^EP2028 (FlyBase, 1992-) P{EP}EP2028 (FlyBase, 1992-) P{EP}garz^EP2028 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 14 )
Research paper	Armbruster and Luschnig, 2012, J. Cell Sci. 125(5): 1318--1328 The Drosophila Sec7 domain guanine nucleotide exchange factor protein Gartenzwerg localizes at the cis-Golgi and is essential for epithelial tube expansion. [FBrf0218010] Wang et al., 2012, J. Cell Sci. 125(2): 461--472 GBF1 (Gartenzwerg)-dependent secretion is required for Drosophila tubulogenesis. [FBrf0217573] Raghu et al., 2009, J. Cell Biol. 185(1): 129--145 Rhabdomere biogenesis in Drosophila photoreceptors is acutely sensitive to phosphatidic acid levels. [FBrf0207819] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Shulman and Feany, 2003, Genetics 165(3): 1233--1242 Genetic modifiers of tauopathy in Drosophila. [FBrf0167629] Kraut et al., 2001, Curr. Biol. 11(6): 417--430 A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135708] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}garzEP2028 Insertion

Dmel\P{EP}garz^EP2028 Insertion