A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}achiEP2107 Insertion

General Information
Symbol Dmel\P{EP}achiEP2107 Species D. melanogaster
Name FlyBase ID FBti0010854
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) achi Viability / fertility
Causes allele(s) achiEP2107 Stock availability 1 publicly available
LINE ID EP(2)2107
Genomic Location
Chromosomal location 2R ( 49A10 ) Sequence location 2R:8,402,313..8,402,313 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0063032 FBti0063033 FBti0050366 FBti0034354 FBti0105598 FBti0152211 FBti0124358 FBti0148488 FBti0072752 FBti0038691 FBti0109277 FBti0047497 FBti0125137_2 FBti0125137_1 FBti0010854 FBti0047982 FBti0113065 FBti0033554 FBti0071077 FBti0125138_2 FBti0125138_1 FBti0007784 FBti0052214 FBti0125139_1 FBti0125139_2
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:8,402,313..8,402,313 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
49A10 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
49A10-49A10 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ072908
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
achi
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
achiEP2107
(Ayyar, 2001.3.5, Ayyar et al., 2003, Gene Disruption Project members, 2001-)
Lethality
References
viable
(Wang and Mann, 2003)
Sterility
References
male sterile | partially
(Wang and Mann, 2003)
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)2107
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
17025
w1118; P{EP}achiEP2107
hide Linkouts
hide Comments
Location 2R:8030715-8030716 confirmed by FlyBase alignment of dbGSS accession AQ072908 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(2)2107
(FlyBase, 1992-)
P{EP}achiEP2107
(FlyBase, 2005, FlyBase, 1992-, )
P{EP}achintyaEP2107
(FlyBase, 1992-)
P{EP}EP2107
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 11 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Ayyar et al., 2003, Development 130(13): 2841--2852
Drosophila TGIF is essential for developmentally regulated transcription in spermatogenesis. [FBrf0160387]
Wang and Mann, 2003, Development 130(13): 2853--2865
Requirement for two nearly identical TGIF-related homeobox genes in Drosophila spermatogenesis. [FBrf0161038]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Ayyar, 2001.3.5, Helping FlyBase: ADRC-10313.
Helping FlyBase: ADRC-10313. [FBrf0135301]
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]