A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}EP2188 Insertion

General Information
Symbol Dmel\P{EP}EP2188 Species D. melanogaster
Name FlyBase ID FBti0010921
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) RpS13 Viability / fertility
Causes allele(s) Stock availability none publicly available
LINE ID EP(2)2188
Genomic Location
Chromosomal location 2L ( 29B1 ) Sequence location 2L:8,362,424..8,362,424 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0143294 FBti0103027 FBti0041992 FBti0036977 FBti0103328 FBti0105417 FBti0106839 FBti0010921 FBti0028630 FBti0039999 FBti0033470 FBti0104488 FBti0104074 FBti0104832 FBti0105589 FBti0033765 FBti0011201 FBti0007657 FBti0105176 FBti0109864 FBti0037387 FBti0028496 FBti0146083 FBti0106013 FBti0109554 FBti0028345 FBti0069650 FBti0011216 FBti0006391 FBti0067917 FBti0050360 FBti0033753 FBti0132316 FBti0003942 FBti0006568 FBti0102931 FBti0071022 FBti0045665 FBti0025183 FBti0124656_2 FBti0124656_1 FBti0025802 FBti0124657_1 FBti0124657_2 FBti0124658_1 FBti0124658_2 FBti0011045 FBti0053825 FBti0047521 FBti0028567 FBti0021682 FBti0011093 FBti0106534 FBti0057252 FBti0068900 FBti0065153 FBti0045327 FBti0047177 FBti0143925
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:8,362,424..8,362,424 [-]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
29B1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
29B2-29B2 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073377
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
RpS13
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)2188
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 2L:8362424-8362425 confirmed by FlyBase alignment of dbGSS accession AQ073377 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(2)2188
(FlyBase, 1992-)
P{EP}EP2188
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]