Dmel\P{EP}wun2^EP2217 Insertion

General Information
Symbol	Dmel\P{EP}wun2^EP2217	Species	D. melanogaster
Name		FlyBase ID	FBti0010947
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	wun2	Viability / fertility
Causes allele(s)	wun2^EP2217	Stock availability	none publicly available
LINE ID	EP(2)2217
Genomic Location
Chromosomal location	2R ( 45D4 )	Sequence location	2R:5,301,691..5,301,691 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:5,301,691..5,301,691 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	45D4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	45D4-45D4 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073405 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	wun2 (BDGP Project Members, 2000-)
Alleles and Phenotypes
Causes alleles	wun2^EP2217 (Starz-Gaiano et al., 2001, Gene Disruption Project members, 2001-)

Lethality References
Sterility References sterile (Starz-Gaiano et al., 2001)
Phenotype Manifest In
	germline cell (Starz-Gaiano et al., 2001)
Detailed Description
Statement Reference Expression of wun2^EP2217 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in defects in germ cell migration in embryos. Expression of wun2^EP2217 under the control of both Scer\GAL4^twi.PG and Scer\GAL4^how-24B results in a strong and completely penetrant germ cell migration phenotype; the germ cells are repelled from their normal target tissue (mesoderm) and instead remain on the midgut. The number of germ cells is reduced in these embryos. Gonadal mesoderm develops normally in these embryos. The embryos develop to adulthood, hatch and are often sterile. Expression of wun2^EP2217 under the control of Scer\GAL4^twi.PG, Scer\GAL4^btl.PS or Scer\GAL4^48Y results in a decrease in germ cell number. (Starz-Gaiano et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2217 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 2R:4925999-4926000 confirmed by FlyBase alignment of dbGSS accession AQ073405 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(2)2217 (FlyBase, 1992-) P{EP}EP2217 (FlyBase, 1992-) P{EP}wun2^EP2217 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Starz-Gaiano et al., 2001, Development 128(6): 983--991 Spatially restricted activity of a Drosophila lipid phosphatase guides migrating germ cells. [FBrf0134584] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}wun2EP2217 Insertion

Dmel\P{EP}wun2^EP2217 Insertion