Dmel\P{EP}Sln^EP2245 Insertion

General Information
Symbol	Dmel\P{EP}Sln^EP2245	Species	D. melanogaster
Name		FlyBase ID	FBti0010970
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Sln	Viability / fertility
Causes allele(s)	Sln^EP2245	Stock availability	none publicly available
LINE ID	EP(2)2245
Genomic Location
Chromosomal location	2R ( 48C4 )	Sequence location	2R:7,732,803..7,732,803 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:7,732,803..7,732,803 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	48C4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	48C5-48C5 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073427 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Sln (BDGP Project Members, 2000-, Jang et al., 2008, Matthews et al., 2009)
Alleles and Phenotypes
Causes alleles	Sln^EP2245 (Jang et al., 2008, Matthews et al., 2009)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Expression of Sln[EP2245] under the control of Scer\GAL4[GMR.PF] produces a normal eye phenotype. Expression of Sln[EP2245] in the developing eye, under the control of Scer\GAL4[GMR.PF] does not affect neuronal differentiation or levels of apoptosis. (Jang et al., 2008) Co-expression of BacA\p35[Scer\UAS.cHa] with both lkb1[Scer\UAS.cLa] and Sln[EP2245] considerably suppresses the apoptotic cell death induced by lkb1[Scer\UAS.cLa] and Sln[EP2245] co-expression in the eye (all under the control of Scer\GAL4[GMR.PF]). (Jang et al., 2008) Down-regulation of Sln through expression of Sln[dsRNA.Sym.Scer\UAS] in the eye dramatically suppresses the narrow eye phenotype induced by lkb1[Scer\UAS.cLa] and Sln[EP2245] (all under the control of Scer\GAL4[GMR.PF]). (Jang et al., 2008)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2245 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 2R:7361205-7361206 confirmed by FlyBase alignment of dbGSS accession AQ073427 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(2)2245 (FlyBase, 1992-, Matthews et al., 2009) P{EP}EP2245 (FlyBase, 1992-, FlyBase, 2005) P{EP}Sln^EP2245 Sln^EP1 (Jang et al., 2008)
Secondary FlyBase IDs

References ( 10 )
Research paper	Matthews et al., 2009, Genetics 181(1): 119--128 Alternative processing of sterol regulatory element binding protein during larval development in Drosophila melanogaster. [FBrf0206893] Jang et al., 2008, J. Cell Biol. 183(1): 11--17 LKB1 induces apical trafficking of Silnoon, a monocarboxylate transporter, in Drosophila melanogaster. [FBrf0205966] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}SlnEP2245 Insertion

Dmel\P{EP}Sln^EP2245 Insertion