Dmel\P{EP}Sir2^EP2300 Insertion

General Information
Symbol	Dmel\P{EP}Sir2^EP2300	Species	D. melanogaster
Name		FlyBase ID	FBti0011018
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	DnaJ-H, Sir2	Viability / fertility
Causes allele(s)	DnaJ-H^EP2300, Sir2^EP2300	Stock availability	1 publicly available
LINE ID	EP(2)2300
Genomic Location
Chromosomal location	2L ( 34A7 )	Sequence location	2L:13,165,564..13,165,571 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:13,165,564..13,165,571 [+] 2L:13,165,561..13,165,561 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	34A7 ( inferred by FlyBase from sequence location )
Cytological location (reported)	34A8-34A8 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073472 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	DnaJ-H (FlyBase, 1996-) Sir2 (BDGP Project Members, 2000-, Ghosh and Feany, 2004, Burnett et al., 2011)
Alleles and Phenotypes
Causes alleles	DnaJ-H^EP2300 (FlyBase, 1996-) Sir2^EP2300 (Fernandez-Funez et al., 2000, Rosenberg and Parkhurst, 2002, Gene Disruption Project members, 2001-, Rogina and Helfand, 2004, Furuyama et al., 2004, FlyBase, 1996-, Ghosh and Feany, 2004, Burnett et al., 2011)

Lethality References viable (Furuyama et al., 2004)
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference Flies expressing Sir2[EP2300] under the control of Scer\GAL4[tub.PU] show no increase in lifespan compared to the control carrying the Scer\GAL4[tub.PU] driver alone. (Burnett et al., 2011) The increased average lifespan of Rpd3^def24 homozygous flies is suppressed by Sir2^EP2300/+. (Rogina and Helfand, 2004) Sir2^EP2300; Scer\GAL4^αTub84B.PL females live on average 57% longer than controls and have a maximal life span (mean of 10% survival) of 19% longer than controls. For males of this genotype, the equivalent figures are 32% and 14% respectively. These lifespan increases are somewhat reduced by a low calorie diet. Similar increases in lifespan are seen when Scer\GAL4^elav-C155 is used as a driver in stead of Scer\GAL4^αTub84B.PL: female average lifespan up 52% and maximal lifespan up 19%; male average lifespan up 20% and maximal lifespan up 8%. Lifespan is not further increased in these flies by a low calorie diet. When Scer\GAL4^arm.PS is used as the driver, there is only a 10-20% increase in average lifespan and no increase in maximal lifespan compared to controls. No increase in lifespan is seen when the driver is Scer\GAL4^D42. The presence of 200μM RU486 in the food of Sir2^EP2300; Scer\GAL4^{elav.Switch.PO} females increases average lifespan by 5-12% and maximal lifespan by 9-16%. (Rogina and Helfand, 2004) The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not modified if the flies are also carrying Sir2^EP2300. (Shulman and Feany, 2003) Expression of Sir2^EP2300 under the control of Scer\GAL4^GMR.PF does not result in a rough eye phenotype. (Shulman and Feany, 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2300 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	24859 w¹¹¹⁸; P{EP}Sir2^EP2300 DnaJ-H^EP2300/CyO
Linkouts
Comments
	Location 2L:13165561-13165562 confirmed by FlyBase alignment of dbGSS accession AQ073472 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999) 8 bp insertion associated host repeat; FBrf0131287 reports that the insertion is 427bp upstream of the putative ATG start codon
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(2)2300 (Ghosh and Feany, 2004) EP-UAS-dSir2 (Burnett et al., 2011) P{EP}EP2300 (FlyBase, 1992-) P{EP}Sir2^EP2300 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 16 )
Research paper	Burnett et al., 2011, Nature 477(7365): 482--485 Absence of effects of Sir2 overexpression on lifespan in C. elegans and Drosophila. [FBrf0215823] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Furuyama et al., 2004, Curr. Biol. 14(20): 1812--1821 SIR2 is required for polycomb silencing and is associated with an E(Z) histone methyltransferase complex. [FBrf0180067] Ghosh and Feany, 2004, Hum. Mol. Genet. 13(18): 2011--2018 Comparison of pathways controlling toxicity in the eye and brain in Drosophila models of human neurodegenerative diseases. [FBrf0180337] Rogina and Helfand, 2004, Proc. Natl. Acad. Sci. U.S.A. 101(45): 15998--16003 Sir2 mediates longevity in the fly through a pathway related to calorie restriction. [FBrf0180651] Shulman and Feany, 2003, Genetics 165(3): 1233--1242 Genetic modifiers of tauopathy in Drosophila. [FBrf0167629] Rosenberg and Parkhurst, 2002, Cell 109(4): 447--458 Drosophila Sir2 is required for heterochromatic silencing and by euchromatic hairy/E(Spl) bHLH repressors in segmentation and sex determination. [FBrf0148924] Fernandez-Funez et al., 2000, Nature 408(6808): 101--106 Identification of genes that modify ataxin-1-induced neurodegeneration. [FBrf0131287] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}Sir2EP2300 Insertion

Dmel\P{EP}Sir2^EP2300 Insertion