Dmel\P{EP}Pp2A-29B^EP2332 Insertion

General Information
Symbol	Dmel\P{EP}Pp2A-29B^EP2332	Species	D. melanogaster
Name		FlyBase ID	FBti0011045
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Pp2A-29B	Viability / fertility
Causes allele(s)	Pp2A-29B^EP2332	Stock availability	2 publicly available
LINE ID	EP(2)2332
Genomic Location
Chromosomal location	2L ( 29B2 )	Sequence location	2L:8,366,028..8,366,028 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10	References Wang et al., 2011, PLoS Genet. 7(8): e1002227
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:8,366,028..8,366,028 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	29B2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	29B3-29B3 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073499 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Pp2A-29B (BDGP Project Members, 2000-, Oren-Giladi et al., 2008, Wang et al., 2011)
Alleles and Phenotypes
Causes alleles	Pp2A-29B^EP2332 (FlyBase, 1996-, Oren-Giladi et al., 2008, Wang et al., 2011)

Lethality References lethal \| recessive (Kotadia et al., 2008)
Sterility References
Phenotype Manifest In
	neuroblast (Wang et al., 2009)
Detailed Description
Statement Reference The embryos resulting from the cross between polo[11]/+, Pp2A-29B[EP2332]/+ mothers and wild-type fathers hatch at wild-type rates. (Wang et al., 2011) The mitotic spindle is misaligned with the cortical crescent in some Pp2A-29B[CB-5426-3]/Pp2A-29B[EP2332] neuroblasts. (Wang et al., 2009)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2332 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 2 )
Bloomington	17044 w¹¹¹⁸; P{EP}Pp2A-29B^EP2332/CyO
Kyoto	114447 y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{EP}Pp2A-29B^EP2332 P{neoFRT}40A/CyO y⁺
Linkouts
Comments
	Location 2L:8366028-8366029 confirmed by FlyBase alignment of dbGSS accession AQ073499 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(2)2332 (FlyBase, 1992-) P{EP}CSN8^EP2332 (FlyBase, 2005, FlyBase, 1992-) P{EP}EP2332 (FlyBase, 1992-) P{EP}Pp2A-29B^EP2332 (FlyBase, 1992-) P{EP}Pp2A^EP2332 (Oren-Giladi et al., 2008) Pp2A-29B^EP2332 (Wang et al., 2011)
Secondary FlyBase IDs

References ( 13 )
Research paper	Wang et al., 2011, PLoS Genet. 7(8): e1002227 PP2A-Twins Is Antagonized by Greatwall and Collaborates with Polo for Cell Cycle Progression and Centrosome Attachment to Nuclei in Drosophila Embryos. [FBrf0214683] Wang et al., 2009, Development 136(13): 2287--2296 Protein phosphatase 2A regulates self-renewal of Drosophila neural stem cells. [FBrf0208148] Kotadia et al., 2008, Oncogene 27(49): 6334--6346 PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen. [FBrf0206924] Oren-Giladi et al., 2008, Genes Cells 13(3): 221--231 Cop9 signalosome subunit 8 (CSN8) is essential for Drosophila development. [FBrf0203123] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{EP}Pp2A-29BEP2332 Insertion

Dmel\P{EP}Pp2A-29B^EP2332 Insertion