Dmel\P{EP}GLaz^EP2383 Insertion

General Information
Symbol	Dmel\P{EP}GLaz^EP2383	Species	D. melanogaster
Name		FlyBase ID	FBti0011086
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	GLaz, Spt-I	Viability / fertility
Causes allele(s)	GLaz^EP2383	Stock availability	none publicly available
LINE ID	EP(2)2383
Genomic Location
Chromosomal location	2R ( 49F4 )	Sequence location	2R:9,055,536..9,055,536 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	2R:9,055,536..9,055,536 [-] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	49F4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	49F7-49F7 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073542 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	GLaz (Walker et al., 2006, Walker, 2006) Spt-I (BDGP Project Members, 2000-)
Alleles and Phenotypes
Causes alleles	GLaz^EP2383 (Walker et al., 2006, Walker, 2006)

Lethality References
Sterility References
Phenotype Manifest In

Detailed Description
Statement Reference GLaz^EP2383 induction, under the control of Scer\GAL4^da.G32 results in greater resistance to hyperoxia. GLaz^EP2383 induction, under the control of Scer\GAL4^da.G32 leads to an increase in mean lifespan at 29^oC of 18%. Expression of GLaz^EP2383, under the control of Scer\GAL4^D42, Scer\GAL4^Act5C or Scer\GAL4^elav-C155 at 29^oC does not have any beneficial effect on lifespan, although expression under the control of Scer\GAL4^hs.PB gives a notable lifespan extension (21% increase over wild-type). Expression of GLaz^EP2383 in the central nervous system and some muscles, under the control of Scer\GAL4^12.1 at 29^oC results in an 18% increase in lifespan. Expression of GLaz^EP2383 in the central nervous system and some muscles, under the control of Scer\GAL4^109(2)80 at 29^oC results in an 29% increase in lifespan. Expression of GLaz^EP2383 in the fat and some muscles, under the control of Scer\GAL4^Lk6-DJ634 at 29^oC results in an 17% increase in lifespan. Expression of GLaz^EP2383 in the fat and some muscles, under the control of Scer\GAL4^how-24B at 29^oC results in an 10% increase in lifespan. Expression of GLaz^EP2383 in the eye and salivary glands, under the control of Scer\GAL4^GMR.PF at 29^oC results in an 22% increase in lifespan. (Walker et al., 2006)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2383 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP2383 (Walker et al., 2006, Walker, 2006) EP(2)2383 (FlyBase, 1992-) GLaz^EP2383 (Sanchez, 2006) P{EP}EP2383 (FlyBase, 1992-) P{EP}GLaz^EP2383 (FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 10 )
Research paper	Walker et al., 2006, Curr. Biol. 16(7): 674--679 Overexpression of a Drosophila homolog of apolipoprotein D leads to increased stress resistance and extended lifespan. [FBrf0190050] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material	Sanchez, 2006, Curr. Biol. 16(7): [FBrf0199311] Walker, 2006, Curr. Biol. 16(7): [FBrf0198586]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}GLazEP2383 Insertion

Dmel\P{EP}GLaz^EP2383 Insertion