A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}hppyEP2445 Insertion

General Information
Symbol Dmel\P{EP}hppyEP2445 Species D. melanogaster
Name FlyBase ID FBti0011138
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) hppy Viability / fertility
Causes allele(s) hppyEP2445 Stock availability none publicly available
LINE ID EP(2)2445
Genomic Location
Chromosomal location 2R ( 56C1 ) Sequence location 2R:15,066,218..15,066,218 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0011138 FBti0105701 FBti0055093 FBti0024509 FBti0107458 FBti0110964 FBti0071173 FBti0027967 FBti0025945 FBti0051600 FBti0064772 FBti0046494 FBti0150154 FBti0048965
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:15,066,218..15,066,218 [+]
(FlyBase, 2005)
Orientation
Cytological location
(computed by FlyBase)
56C1 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
56C1-56C1 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
56C1-56C2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ254864
(FlyBase, 1992-)
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
hppy
(BDGP Project Members, 2000-, Raymond et al., 2004)
hide Alleles and Phenotypes
Causes alleles
hppyEP2445
(Raymond et al., 2004)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)2445
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 2R:14693575-14693576 determined by FlyBase alignment of dbGSS accession AQ254864 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(2)2445
(Bradley and Andrew, 2001, FlyBase, 1992-, Raymond et al., 2004)
P{EP}EP2445
(FlyBase, 2005, FlyBase, 1992-)
P{EP}hppyEP2445
 
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Raymond et al., 2004, J. Cell Sci. 117(13): 2777--2789
A screen for modifiers of RacGAP(84C) gain-of-function in the Drosophila eye revealed the LIM kinase Cdi/TESK1 as a downstream effector of Rac1 during spermatogenesis. [FBrf0179402]
Bradley and Andrew, 2001, Development 128(15): 3001--3015
ribbon encodes a novel BTB/POZ protein required for directed cell migration in Drosophila melanogaster. [FBrf0138367]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]