A Database of Drosophila Genes & Genomes

FB2012_01, released January 20th, 2012
 

Dmel\P{EP}Dap160EP2543 Insertion

General Information
Symbol Dmel\P{EP}Dap160EP2543 Species D. melanogaster
Name FlyBase ID FBti0011223
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) Dap160 Viability / fertility
Causes allele(s) Dap160EP2543 Stock availability 1 publicly available
LINE ID EP(2)2543
Genomic Location
Chromosomal location 2L ( 39A4 ) Sequence location 2L:21,141,902..21,141,902 [-]
Map ( GBrowse ) detailed view FBti0019827 FBti0019828 FBti0048971 FBti0050471 FBti0108593 FBti0011158 FBti0011223 FBti0071049 FBti0100073 FBti0102222 FBti0124897_2 FBti0124897_1 FBti0026226 FBti0039673 FBti0023158 FBti0107828 FBti0041560 FBti0124898 FBti0025090 FBti0025696 FBti0104531 FBti0048231 FBti0036517 FBti0023600 FBti0033508 FBti0054668
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Description
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FB2012_01
FB2011_10
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:21,141,902..21,141,902 [-]
(BDGP Project Members, 2000-)
Orientation
?
(Lloyd et al., 2000)
Cytological location
(computed by FlyBase)
39A4 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
39B1-39B1 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073674
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Dap160
(BDGP Project Members, 2000-, Lloyd et al., 2000, Kelly and Phillips, 2005, Tang et al., 2005, Robertson et al., 2000)
hide Alleles and Phenotypes
Causes alleles
Dap160EP2543
(Gene Disruption Project members, 2001-, Marie et al., 2004, Koh et al., 2004, Kelly and Phillips, 2005, Tang et al., 2005, Robertson et al., 2000)
Lethality
References
viable
(Marie et al., 2004)
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
Homozygotes are phenotypically wild-type.
(Kelly and Phillips, 2005)
stnA6/Y ; Dap160EP2543/Df(2L)TW65 males are viable but are somewhat late to eclose and are extremely uncoordinated as adults, dying within 48 hours of eclosion. stnB15/Y ; Dap160EP2543/Df(2L)TW65 males show about 35% survival compared to control siblings. The surviving adults are sedentary, extremely uncoordinated and exhibit an extreme stress-sensitive phenotype. They die within 3 days of eclosion.
(Kelly and Phillips, 2005)
Overexpression of Dap160[EP2468] under the control of Scer\GAL4[GMR.PF] produces a phenotypically wild-type wing.
(Robertson et al., 2000)
Co-expression of Cbl[Dv.Scer\UAS] with Dap160[EP2468], both under the control of Scer\GAL4[GMR.PF] results in an increase in ectopic wing veins compared to expression of Cbl[Dv.Scer\UAS] alone.
(Robertson et al., 2000)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(2)2543
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 1 )
Bloomington
19582
w1118; P{EP}Dap160EP2543
hide Linkouts
hide Comments
Location 2L:21129495-21129496 confirmed by FlyBase alignment of dbGSS accession AQ073674 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP2543
(Tang et al., 2005)
EP(2)2543
(Koh et al., 2004, Marie et al., 2004, Lloyd et al., 2000, FlyBase, 1992-, Kelly and Phillips, 2005)
EP{2}EP2543Dap160
(Robertson et al., 2000)
P{EP}Dap160EP2543
(FlyBase, 2005, FlyBase, 1992-)
P{EP}EP2543
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 14 )
Research paper
Kelly and Phillips, 2005, Biochem. J. 388(1): 195--204
Molecular and genetic characterization of the interactions between the Drosophila stoned-B protein and DAP-160 (intersectin). [FBrf0187146]
Tang et al., 2005, Molec. Cell. Biol. 25(8): 2899--2909
Numb Proteins Specify Asymmetric Cell Fates via an Endocytosis- and Proteasome-Independent Pathway. [FBrf0184155]
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Koh et al., 2004, Neuron 43(2): 193--205
Dap160/Intersectin acts as a stabilizing scaffold required for synaptic development and vesicle endocytosis. [FBrf0179283]
Marie et al., 2004, Neuron 43(2): 207--219
Dap160/Intersectin scaffolds the periactive zone to achieve high-fidelity endocytosis and normal synaptic growth. [FBrf0179322]
Lloyd et al., 2000, Neuron 26(1): 45--50
A genome-wide search for synaptic vesicle cycle proteins in Drosophila. [FBrf0127207]
Robertson et al., 2000, Oncogene 19(29): 3299--3308
A Drosophila analogue of v-Cbl is a dominant-negative oncoprotein in vivo. [FBrf0130045]
Spradling et al., 1999, Genetics 153(1): 135--177
The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation
FlyBase curation. [FBrf0105495]
Computer file
Gene Disruption Project members, 2001-, [title not yet available]
[title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0125078]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.
Berkeley Drosophila Genome Project. [FBrf0067338]