Dmel\P{EP}lea^EP2582 Insertion

General Information
Symbol	Dmel\P{EP}lea^EP2582	Species	D. melanogaster
Name		FlyBase ID	FBti0011255
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	lea	Viability / fertility
Causes allele(s)	lea^EP2582	Stock availability	1 publicly available
LINE ID	EP(2)2582
Genomic Location
Chromosomal location	2L ( 22A1 )	Sequence location	2L:1,420,531..1,420,531 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	2L:1,420,531..1,420,531 [-] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	22A1 ( inferred by FlyBase from sequence location )
Cytological location (reported)	22A1-22A2 (in situ hybridization reported) (BDGP Project Members, 1994-1999) 22A2-22A2 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ254875 (FlyBase, 1992-)
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	lea (Simpson et al., 2000, Zhu et al., 2005, McGovern et al., 2003, Couch et al., 2004, Jhaveri et al., 2004, Simpson et al., 2000, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Gene Disruption Project members, 2001-, Kraut and Zinn, 2004, Kraut et al., 2001, Kraut et al., 2001)
Alleles and Phenotypes
Causes alleles	lea^EP2582 (Simpson et al., 2000, Zhu et al., 2005, McGovern et al., 2003, Couch et al., 2004, Jhaveri et al., 2004, Simpson et al., 2000, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Gene Disruption Project members, 2001-, Kraut and Zinn, 2004, Kraut et al., 2001, Kraut et al., 2001)

Lethality References lethal (Rajagopalan et al., 2000) partially lethal - majority die (McGovern et al., 2003)
Sterility References
Phenotype Manifest In
	abdominal lateral pentascolopidial chordotonal organ lch5 (Kraut and Zinn, 2004) abdominal lateral pentascolopidial chordotonal organ lch5 & scolopidial dendrite (Kraut and Zinn, 2004) abdominal lateral pentascolopidial chordotonal organ lch5 & scolopidial dendritic cap cell (Kraut and Zinn, 2004) antennal commissure (Jhaveri et al., 2004) antennal lobe glomerulus (Jhaveri et al., 2004) cell body & serotonin neuron (Couch et al., 2004) dMP2 neuron (Simpson et al., 2000) intersegmental nerve (Kraut et al., 2001) serotonin neuron & ventral nerve cord (Couch et al., 2004) ventral nerve cord commissure (McGovern et al., 2003, Rajagopalan et al., 2000) vMP2 neuron (Simpson et al., 2000)
Detailed Description
Statement Reference In lea^EP2582; Scer\GAL4^eg-Mz360 embryos, serotonergic axons do not cross the midline. Despite their defects in serotonergic neuronal axon guidance, these neurons retain their normal ability to take up serotonin. Unlike wild-type serotonergic neurons, the ability of serotonergic neurons in the ventral nerve cord of stage 16 lea^EP2582; Scer\GAL4^eg-Mz360 embryos to take up serotonin is not lost when their axonal contacts with the midline are severed. The cell bodies of serotonergic neurons in the ventral nerve cord of stage 16 lea^EP2582; Scer\GAL4^eg-Mz360 embryos are shifted laterally compared to wild-type. (Couch et al., 2004) Commissure formation and glomerular morphology in the antennal lobes of lea^EP2582; Scer\GAL4^SG18.1 flies are restored by Df(2R)WMG/+. (Jhaveri et al., 2004) Antennal commissure formation is abolished and glomerular morphology is disrupted in the antennal lobes of lea^EP2582; Scer\GAL4^SG18.1 flies. (Jhaveri et al., 2004) The transformation of abdominal lch5 chordotonal organs to a morphology resembling thoracic dch3 chordotonal organs that is seen in larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155 is still seen if they are also mutant for robo⁷; 36.4% of abdominal segments have partial or complete transformation of the lch5 organs in the double mutant larvae. The transformation of abdominal lch5 chordotonal organs to a morphology resembling thoracic dch3 chordotonal organs that is seen in larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155 is attenuated if the larvae also carry one copy of sli²; only 17.2% of abdominal segments have partial or complete transformation of the lch5 organs in the double mutant larvae at 25^oC. (Kraut and Zinn, 2004) Abdominal lch5 chordotonal organs are transformed so that they resemble thoracic dch3 chordotonal organs in larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155. The transformed "lch5" chordotonal organs are displaced dorsally in the ectoderm. They have the usual number of five neurons per cluster, but their dendrites and support cells show a variety of orientations, which range from the ventral orientation typical of dch3 organs, through intermediate bent orientations, to orientations similar to those of normal lch5 organs. At 29^oC, 44% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology. At 25^oC, 32% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology. Defects indicating transformation of the lch5 chordotonal organs can be detected as early as stage 14 in embryos expressing lea^EP2582 under the control of Scer\GAL4^elav-C155. The cap cells of the transformed "lch5" organs always follow the orientation of the transformed neurons. At 29^oC, 30.5% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology towards that of the thoracic dch3 chordotonal organs, in larvae expressing lea^EP2582 under the control of Scer\GAL4^ato.3.6. Some abdominal lch5 chordotonal organs fail to migrate ventrally (as occurs in wild type) in embryos expressing lea^EP2582 under the control of Scer\GAL4^ato.3.6. Some of these "stalled" abdominal clusters extend projections dorsally. At 29^oC, 17% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology towards that of the thoracic dch3 chordotonal organs, in embryos expressing lea^EP2582 under the control of Scer\GAL4^48Y. (Kraut and Zinn, 2004) ventral nerve cord commissures in stage 17 lea^EP2582; Scer\GAL4^sca-4512 embryos are missing or fused. (McGovern et al., 2003) Mutant larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. (Kraut et al., 2001) When expression of lea^EP2582 is driven by Scer\GAL4^elav.PLu, commissures are lost. (Rajagopalan et al., 2000) Expression of lea^EP2582 under the control of Scer\GAL4^ap-md544 causes ap-expressing neurons to move laterally (relative to wild type) and extend anteriorly in a specific location between the intermediate and lateral Fas2-expressing pathways. The ap-expressing axons from neighbouring segments appear to pick the same lateral pathway and to fasciculate together as they extend anteriorly from segment to segment. Expression of lea^EP2582 under the control of Scer\GAL4^15J2 results in a bimodal phenotype. The dMP2 and vMP2 axons always appear to extend in a Fas2-expressing pathway, but they are now found in either the intermediate or lateral pathways (they normally extend in the medial pathway). They are usually found in the intermediate pathway and occasionally found in the lateral pathway. (Simpson et al., 2000)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(2)2582 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17071 w¹¹¹⁸; P{EP}lea^EP2582
Linkouts
Comments
	Location 2L:1420530-1420531 determined by FlyBase alignment of dbGSS accession AQ254875 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP2582 (Zhu et al., 2005, Couch et al., 2004, Jhaveri et al., 2004, Kraut and Zinn, 2004) EP(2)2582 (McGovern et al., 2003, Kraut et al., 2001, Kraut et al., 2001, FlyBase, 1992-) EP-robo2 (Kraut and Zinn, 2004) P{EP}EP2582 (FlyBase, 1992-) P{EP}lea^EP2582 (FlyBase, 2005, FlyBase, 1992-, ) P{EP}robo2^EP2582 (FlyBase, 1992-) UAS-robo2 (Jhaveri et al., 2004)
Secondary FlyBase IDs

References ( 19 )
Research paper	Zhu et al., 2005, Genetics 170(2): 767--777 A screen for genes that influence fibroblast growth factor signal transduction in Drosophila. [FBrf0187664] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Couch et al., 2004, Development 131(5): 997--1006 robo2 and robo3 interact with eagle to regulate serotonergic neuron differentiation. [FBrf0174592] Jhaveri et al., 2004, Development 131(9): 1903--1912 Positioning sensory terminals in the olfactory lobe of Drosophila by Robo signaling. [FBrf0174568] Kraut and Zinn, 2004, Curr. Biol. 14(15): 1319--1329 Roundabout 2 regulates migration of sensory neurons by signaling in trans. [FBrf0180051] McGovern et al., 2003, Mech. Dev. 120(10): 1193--1207 A targeted gain of function screen in the embryonic CNS of Drosophila. [FBrf0167840] Kraut et al., 2001, Curr. Biol. 11(6): 417--430 A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135708] Rajagopalan et al., 2000, Neuron 28(3): 767--777 Crossing the midline: roles and regulation of Robo receptors. [FBrf0132432] Rajagopalan et al., 2000, Cell 103(7): 1033--1045 Selecting a longitudinal pathway: robo receptors specify the lateral position of axons in the Drosophila CNS. [FBrf0132258] Simpson et al., 2000, Cell 103(7): 1019--1032 Short-range and long-range guidance by Slit and its Robo receptors: a combinatorial code of Robo receptors controls lateral position. [FBrf0132257] Simpson et al., 2000, Neuron 28(3): 753--766 Short-range and long-range guidance by Slit and its Robo receptors: robo and Robo2 play distinct roles in midline guidance. [FBrf0132431] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}leaEP2582 Insertion

Dmel\P{EP}lea^EP2582 Insertion