A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}fabpEP3252 Insertion

General Information
Symbol Dmel\P{EP}fabpEP3252 Species D. melanogaster
Name FlyBase ID FBti0011422
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) fabp, sea Viability / fertility
Causes allele(s) fabpEP3252, seaEP3252 Stock availability none publicly available
LINE ID EP(3)3252
Genomic Location
Chromosomal location 3R ( 86E10 ) Sequence location 3R:7,392,664..7,392,664 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0020329 FBti0041203 FBti0075900 FBti0075011 FBti0054496 FBti0042997 FBti0011514 FBti0051416 FBti0108478 FBti0055363 FBti0059595 FBti0104771 FBti0143518 FBti0143845 FBti0108952 FBti0105167 FBti0099763 FBti0110193 FBti0024569 FBti0108863 FBti0059622 FBti0113167 FBti0107304 FBti0068508 FBti0011575 FBti0027149 FBti0011422 FBti0111958 FBti0069410 FBti0109161 FBti0039869 FBti0034795 FBti0105702 FBti0110385 FBti0033741 FBti0108276 FBti0054222 FBti0007887 FBti0046320 FBti0007854 FBti0105215 FBti0068430 FBti0068692 FBti0054848 FBti0103295 FBti0066626 FBti0047900 FBti0109322 FBti0007822 FBti0068548 FBti0068559 FBti0034508 FBti0108720 FBti0034013 FBti0103408 FBti0033481 FBti0111606 FBti0100861 FBti0110302 FBti0011322 FBti0113277 FBti0107255 FBti0011356 FBti0011394 FBti0108273 FBti0029531 FBti0099986 FBti0035539 FBti0103637 FBti0102871 FBti0070388 FBti0070326 FBti0037136 FBti0037139 FBti0033529 FBti0059214 FBti0033939 FBti0100851 FBti0011484 FBti0111970 FBti0105658 FBti0068422 FBti0057478 FBti0033547 FBti0105765 FBti0035932 FBti0069269 FBti0029989 FBti0057360 FBti0030131 FBti0112016 FBti0043538 FBti0011743 FBti0104892 FBti0106698 FBti0036621 FBti0030244 FBti0059435 FBti0021617 FBti0109014 FBti0029409 FBti0104647 FBti0034409 FBti0055828 FBti0106710 FBti0037118 FBti0072517 FBti0028670 FBti0107586 FBti0043117 FBti0127449 FBti0107999
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:7,392,664..7,392,664 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
86E10 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
86E14-86E14 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073857
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
fabp
(BDGP Project Members, 2000-)
sea
(Gene Disruption Project members, 2001-, FlyBase Genome Annotators, 2002-2003)
hide Alleles and Phenotypes
Causes alleles
fabpEP3252
(Gene Disruption Project members, 2001-, FlyBase Genome Annotators, 2002-2003, Schulz et al., 2004)
seaEP3252
(Gene Disruption Project members, 2001-, FlyBase Genome Annotators, 2002-2003)
Lethality
References
Sterility
References
hide Phenotype Manifest In
germline cell | male
(Schulz et al., 2004)
hide Detailed Description
Statement
Reference
Expression of CG6783EP3252 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 (the animals are initially raised at 18oC and are then shifted to 29oC after 7 days) results in a reduced number of early germ cells (stem cells, gonialblasts and spermatogonia) in the testis. Expression of CG6783EP3252 under the control of Scer\GAL4ptc-559.1 (the animals are initially raised at 18oC and then shifted to 29oC after 10 days) results in a reduced number of early germ cells (stem cells, gonialblasts and spermatogonia) in the testis.
(Schulz et al., 2004)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)3252
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3R:7392664-7392665 confirmed by FlyBase alignment of dbGSS accession AQ073857 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(3)3252
(Schulz et al., 2004, FlyBase, 1992-)
P{EP}CG6783EP3252
(FlyBase, 1992-, FlyBase, 2005)
P{EP}EP3252
(FlyBase, 1992-)
P{EP}fabpEP3252
 
Secondary FlyBase IDs
hide References ( 10 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Schulz et al., 2004, Genetics 167(2): 707--723
A misexpression screen reveals effects of bag-of-marbles and TGF beta class signaling on the Drosophila male germ-line stem cell lineage. [FBrf0179436]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome.
Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]