A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}EP3283EP3283 Insertion

General Information
Symbol Dmel\P{EP}EP3283EP3283 Species D. melanogaster
Name FlyBase ID FBti0011448
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) EP3283, sba Viability / fertility
Causes allele(s) EP3283EP3283 Stock availability none publicly available
LINE ID EP(3)3283
Genomic Location
Chromosomal location 3R ( 95C12 ) Sequence location 3R:19,748,774..19,748,774 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBti0148487 FBti0055766 FBti0076109 FBti0048848 FBti0150438 FBti0108361 FBti0076094 FBti0029509 FBti0011449 FBti0011448 FBti0011649 FBti0025274 FBti0130171 FBti0078224 FBti0102693 FBti0132309 FBti0126237 FBti0130167 FBti0037670 FBti0038832
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:19,748,774..19,748,774 [-]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
95C12 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
95C11-95C11 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073882
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
EP3283
(Pena-Rangel et al., 2002)
sba
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
EP3283EP3283
(Pena-Rangel et al., 2002)
Lethality
References
Sterility
References
hide Phenotype Manifest In
wing margin bristle
(Pena-Rangel et al., 2002)
wing vein | supernumerary
(Pena-Rangel et al., 2002)
hide Detailed Description
Statement
Reference
pnrMD237/+ adults expressing EP3283EP3283 under the control of Scer\GAL4pnr-MD237 have a slight thoracic cleft and a reduced scutellum.
(Pena-Rangel et al., 2002)
Flies expressing EP3283EP3283 under the control of Scer\GAL4ap-md544 have extra wing veins and alterations of chaetae in the anterior wing margin.
(Pena-Rangel et al., 2002)
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)3283
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3R:19748774-19748775 confirmed by FlyBase alignment of dbGSS accession AQ073882 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
P{EP}EP3283
(FlyBase, 1992-)
P{EP}EP3283EP3283
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Pena-Rangel et al., 2002, Genetics 160(3): 1035--1050
A misexpression study examining dorsal thorax formation in Drosophila melanogaster. [FBrf0147055]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]