Dmel\P{EP}Atg1^EP3348 Insertion

General Information
Symbol	Dmel\P{EP}Atg1^EP3348	Species	D. melanogaster
Name		FlyBase ID	FBti0011500
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Atg1, Sap130	Viability / fertility
Causes allele(s)	Atg1^EP3348, Sap130^EP3348	Stock availability	none publicly available
LINE ID	EP(3)3348
Genomic Location
Chromosomal location	3L ( 69E4 )	Sequence location	3L:12,798,083..12,798,083 [+]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:12,798,083..12,798,083 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	69E4 ( inferred by FlyBase from sequence location )
Cytological location (reported)	69E2-69E2 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073934 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Atg1 (BDGP Project Members, 2000-, Toda et al., 2008, Cao et al., 2008) Sap130 (Cao et al., 2008)
Alleles and Phenotypes
Causes alleles	Atg1^EP3348 (Gene Disruption Project members, 2001-, Zhu et al., 2005, Scott et al., 2004, Toda et al., 2008, Cao et al., 2008) Sap130^EP3348 (Cao et al., 2008)

Lethality References lethal \| pupal stage \| recessive (Scott et al., 2004) partially lethal - majority die (Lee et al., 2007) partially lethal - majority die \| adult stage (Toda et al., 2008)
Sterility References
Phenotype Manifest In
	commissure (Ahantarig et al., 2009) longitudinal connective (Ahantarig et al., 2009)
Detailed Description
Statement Reference Heterozygous embryos have distorted and broken longitudinal connectives. Homozygous embryos show increased distortion of the commissures and longitudinal connectives. (Ahantarig et al., 2009) P{EP}Atg1[EP3348] heterozygous embryos have distorted and broken longitudinal connectives. P{EP}Atg1[EP3348] homozygous embryos show increased distortion of the commissures and longitudinal connectives. (Ahantarig et al., 2009) Expression using Scer\GAL4[ey.PU] does not induce a rough eye phenotype. (Cao et al., 2008) About 30% of Atg1[EP3348] homozygous mutants develop into adults. No mitotic defects are evident in the larval brain and imaginal discs of mutant animals. Atg1[EP3348] mutant animals show marked defects in the induction of autophagy and autophagosome formation under conditions of starvation. (Lee et al., 2007) Defects in autophagosome formation in starved Atg1[EP3348] homozygous larvae are not rescued in a S6k[07084] or S6k[l-1] heterozygous background. However, eclosion rates are increased in these animals in a dose-dependent manner. Roughly 50% of Atg1[EP3348], S6k[07084]/+ eclose, increased to 70% in a Atg1[EP3348], S6k[l-1]/+ genetic background. Tor[k17004] homozygous animals in a heterozygous Atg1[EP3348] genetic background grow faster, and extend their developmental stage to mid/late third instar larval stage. Double homozygous animals survive to mid-late third instar larval stage. Lipid vesicle aggregation in the fat body of Tor[k17004] homozygous second/early third instar larvae is suppressed in a heterozygous Atg1[EP3348] genetic background. Tor[k17004] mutant larvae heterozygous for Atg1[EP3348] show a partially rescued cell and nuclear salivary gland size. (Lee et al., 2007) Mutants show disruption of autophagy. (Scott et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3348 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 3L:12778930-12778931 confirmed by FlyBase alignment of dbGSS accession AQ073934 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP3348 (Zhu et al., 2005) EP(3)3348 (FlyBase, 1992-, Toda et al., 2008, Ahantarig et al., 2009, Cao et al., 2008) P{EP}Atg1^EP3348 (FlyBase, 2005, FlyBase, 1992-, ) P{EP}CG10967^EP3348 (FlyBase, 1992-) P{EP}EP3348 (FlyBase, 1992-) P{EP}l(3)00305^EP3348 (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 14 )
Research paper	Ahantarig et al., 2009, Molec. Biol. Rep. 36(6): 1311--1321 Molecular characterization of Pegarn: a Drosophila homolog of UNC-51 kinase. [FBrf0208017] Cao et al., 2008, Genetics 178(3): 1457--1471 Identification of novel genes that modify phenotypes induced by Alzheimer's beta-amyloid overexpression in Drosophila. [FBrf0205107] Toda et al., 2008, Genes Dev. 22(23): 3292--3307 UNC-51/ATG1 kinase regulates axonal transport by mediating motor-cargo assembly. [FBrf0206363] Lee et al., 2007, EMBO Rep. 8(4): 360--365 ATG1, an autophagy regulator, inhibits cell growth by negatively regulating S6 kinase. [FBrf0201708] Zhu et al., 2005, Genetics 170(2): 767--777 A screen for genes that influence fibroblast growth factor signal transduction in Drosophila. [FBrf0187664] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material	Scott et al., 2004, Dev. Cell 7(2): Supplementary material. [FBrf0183440]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}Atg1EP3348 Insertion

Dmel\P{EP}Atg1^EP3348 Insertion