A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}MenEP3376 Insertion

General Information
Symbol Dmel\P{EP}MenEP3376 Species D. melanogaster
Name FlyBase ID FBti0011522
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) Men Viability / fertility
Causes allele(s) MenEP3376 Stock availability none publicly available
LINE ID EP(3)3376
Genomic Location
Chromosomal location 3R ( 87C7 ) Sequence location 3R:8,545,703..8,545,703 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0129390 FBti0011687 FBti0126058 FBti0037933 FBti0043600 FBti0143726 FBti0049765 FBti0139976 FBti0099821 FBti0035951 FBti0035432 FBti0106088 FBti0110971 FBti0029153 FBti0110780 FBti0007834 FBti0072350 FBti0111277 FBti0106831 FBti0037020 FBti0113022 FBti0036577 FBti0007798 FBti0007808 FBti0104632 FBti0106038 FBti0033750 FBti0034431 FBti0030083 FBti0112491 FBti0070526 FBti0070746 FBti0037472 FBti0028178 FBti0027097 FBti0008009 FBti0077078 FBti0018588 FBti0034396 FBti0011522 FBti0029625 FBti0113223 FBti0069737 FBti0058822 FBti0007839 FBti0007837 FBti0033967 FBti0037081 FBti0103693 FBti0059217 FBti0029978 FBti0029053 FBti0054448 FBti0109949 FBti0106678 FBti0038093 FBti0016457 FBti0143591 FBti0007944 FBti0038092 FBti0146165 FBti0048096 FBti0053413
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:8,545,703..8,545,703 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
87C7 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
87C9-87C9 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073955
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
Men
(BDGP Project Members, 2000-)
hide Alleles and Phenotypes
Causes alleles
MenEP3376
(Gene Disruption Project members, 2001-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)3376
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3R:8545703-8545704 confirmed by FlyBase alignment of dbGSS accession AQ073955 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(3)3376
(FlyBase, 1992-)
P{EP}EP3376
(FlyBase, 1992-)
P{EP}MenEP3376
(FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs
hide References ( 8 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]