Dmel\P{EP}Klp67A^EP3516 Insertion

General Information
Symbol	Dmel\P{EP}Klp67A^EP3516	Species	D. melanogaster
Name		FlyBase ID	FBti0011629
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Klp67A	Viability / fertility
Causes allele(s)	Klp67A^EP3516	Stock availability	1 publicly available
LINE ID	EP(3)3516
Genomic Location
Chromosomal location	3L ( 67B2 )	Sequence location	3L:9,360,016..9,360,016 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:9,360,016..9,360,016 [+] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	67B2 ( inferred by FlyBase from sequence location )
Cytological location (reported)	67B1-67B1 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ072986 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Klp67A (BDGP Project Members, 2000-)
Alleles and Phenotypes
Causes alleles	Klp67A^EP3516 (Gene Disruption Project members, 2001-, Gandhi et al., 2004, Savoian et al., 2004)

Lethality References
Sterility References
Phenotype Manifest In
	meiotic anaphase II & condensed nuclear chromosome \| male (Savoian et al., 2004) mitotic prometaphase & nuclear chromosome \| male (Savoian et al., 2004) spermatocyte & nuclear chromosome (Savoian et al., 2004)
Detailed Description
Statement Reference The chromosomes in Klp67A^EP3516/Df(3L)29A6 mutant primary spermatocytes are abnormally dynamic and undergo exaggerated periods of reorientation during prometaphase. Unlike in wild-type spermatocytes, the chromosomes of mutant spermatocytes form poorly defined metaphase plates, which tend to be staggered with the chromosomes only loosely aligned. Additionally, the mutant chromosomes often do not find stable positions at the spindle equator and may exhibit one or more motions before anaphase onset. Prior to anaphase in the spermatocytes of Klp67A^EP3516/Df(3L)29A6 mutants, homologous chromosomes appear twisted relative to one another; this effect is not seen in wild type. During anaphase, chromosome movement does not occur in a continuous manner and poleward migration velocity is greatly attenuated compared to wild type. In some mutant cells dyads begin the migration poleward but then abruptly halt and remain stationary relative to the spindle pole, exhibiting no further poleward movement before decondensing. In a few cells it appears that disjoined dyads do not undergo any significant anaphase A movement. Nuclear reformation occurs at ectopic non-polar locations in 89% of Klp67A^EP3516/Df(3L)29A6 mutants. (Savoian et al., 2004)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3516 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17269 w¹¹¹⁸; P{EP}Klp67A^EP3516/TM6B, Tb¹
Linkouts
Comments
	Location 3L:9340863-9340864 confirmed by FlyBase alignment of dbGSS accession AQ072986 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3)3516 (FlyBase, 1992-) Klp67A^EP(3)3516 (Savoian et al., 2004) P{EP}EP3516 (FlyBase, 1992-) P{EP}Klp67A^EP3516 (FlyBase, 2005, FlyBase, 1992-)
Secondary FlyBase IDs

References ( 10 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Gandhi et al., 2004, Molec. Biol. Cell 15(1): 121--131 The Drosophila kinesin-like protein KLP67A is essential for mitotic and male meiotic spindle assembly. [FBrf0167863] Savoian et al., 2004, J. Cell Sci. 117(16): 3669--3677 Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I. [FBrf0179430] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{EP}Klp67AEP3516 Insertion

Dmel\P{EP}Klp67A^EP3516 Insertion