Dmel\P{EP}Cnx99A^EP3522 Insertion

General Information
Symbol	Dmel\P{EP}Cnx99A^EP3522	Species	D. melanogaster
Name		FlyBase ID	FBti0011634
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	Cnx99A	Viability / fertility
Causes allele(s)	Cnx99A^EP3522	Stock availability	1 publicly available
LINE ID	EP(3)3522
Genomic Location
Chromosomal location	3R ( 99A7 )	Sequence location	3R:25,145,356..25,145,356 [+]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:25,145,356..25,145,356 [+] (FlyBase, 2005)
Orientation
Cytological location (computed by FlyBase)	99A7 ( inferred by FlyBase from sequence location )
Cytological location (reported)	99A7-99A7 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ072988 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	Cnx99A (BDGP Project Members, 2000-, Franciscovich et al., 2008)
Alleles and Phenotypes
Causes alleles	Cnx99A^EP3522 (McGovern et al., 2003, Franciscovich et al., 2008)

Lethality References lethal (McGovern et al., 2003)
Sterility References
Phenotype Manifest In
	intermediate longitudinal fascicle (McGovern et al., 2003) medial longitudinal fascicle (McGovern et al., 2003) ventral nerve cord (McGovern et al., 2003) ventral nerve cord commissure (McGovern et al., 2003)
Detailed Description
Statement Reference Expression of Cnx99A[EP3522] enhances the small eye phenotype associated with kay[Fbz.Scer\UAS], when both are expressed under the control of Scer\GAL4[ey.3.5.Exel]. Co-expression of Cnx99A[EP3522] with kay[Fbz.Scer\UAS] in differentiated post-mitotic neurons under the control of Scer\GAL4[elav-C155] partially suppresses the small synapse phenotype found in kay[Fbz.Scer\UAS] mutants. (Franciscovich et al., 2008) Ventral nerve cord commissures in stage 17 Cnx99A^EP3522; Scer\GAL4^sca-4512 embryos are fused and/or \'fuzzy\'.The two most medial 1D4 longitudinal fascicles cross and recross the midline. Marker assays suggest some cell fate changes among ventral nerve cord cells. Similar defects in ventral nerve cord commissures and ventral nerve cord cell fate are seen in Cnx99A^EP3522; Scer\GAL4^sim.PS embryos, but only the most medial 1D4 longitudinal fascicle crosses the midline. (McGovern et al., 2003)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3522 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17136 w¹¹¹⁸; P{EP}Cnx99A^EP3522/TM6B, Tb¹
Linkouts
Comments
	Location 3R:25145355-25145356 determined by FlyBase alignment of dbGSS accession AQ072988 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3)3522 (FlyBase, 1992-) EP(3)3552 (McGovern et al., 2003) P{EP}Cnx99A^EP3522 (FlyBase, 2005, FlyBase, 1992-) P{EP}EP3522 (FlyBase, 1992-) unnamed (Franciscovich et al., 2008)
Secondary FlyBase IDs

References ( 10 )
Research paper	Franciscovich et al., 2008, Genetics 180(4): 2057--2071 Overexpression screen in Drosophila identifies neuronal roles of GSK-3 beta/shaggy as a regulator of AP-1-dependent developmental plasticity. [FBrf0207092] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] McGovern et al., 2003, Mech. Dev. 120(10): 1193--1207 A targeted gain of function screen in the embryonic CNS of Drosophila. [FBrf0167840] Spradling et al., 1999, Genetics 153(1): 135--177 The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489] Rorth, 1996, Proc. Natl. Acad. Sci. USA 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
FlyBase analysis	FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078] BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0067338]

Dmel\P{EP}Cnx99AEP3522 Insertion

Dmel\P{EP}Cnx99A^EP3522 Insertion