Dmel\P{EP}JIL-1^EP3657 Insertion

General Information
Symbol	Dmel\P{EP}JIL-1^EP3657	Species	D. melanogaster
Name		FlyBase ID	FBti0011744
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	JIL-1	Viability / fertility
Causes allele(s)	JIL-1^EP3657	Stock availability	none publicly available
LINE ID	EP(3)3657
Genomic Location
Chromosomal location	3L ( 68A5 )	Sequence location	3L:11,085,410..11,085,410 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Detailed Mapping Data
Chromosome (arm)
Sequence Location	3L:11,085,410..11,085,410 [-] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	68A5 ( inferred by FlyBase from sequence location )
Cytological location (reported)	68A3-68A3 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073095 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	JIL-1 (BDGP Project Members, 2000-)
Alleles and Phenotypes
Causes alleles	JIL-1^EP3657 (Kraut et al., 2001, Gene Disruption Project members, 2001-, Wang et al., 2001, Kraut et al., 2001)

Lethality References lethal (Zhang et al., 2003) lethal \| embryonic stage \| maternal effect (Zhang et al., 2003) lethal \| partially \| recessive (Zhang et al., 2003)
Sterility References fertile (Wang et al., 2001)
Phenotype Manifest In
	abdominal sternite 5 \| ectopic \| male (Zhang et al., 2003) abdominal sternite 6 \| male (Zhang et al., 2003) intersegmental nerve (Kraut et al., 2001) ovary (Zhang et al., 2003)
Detailed Description
Statement Reference The hatch rate of lola⁰⁰⁶⁴²/+ ; JIL-1^EP3657/JIL-1^EP3657 embryos is 20%. (Zhang et al., 2003) The hatch rate of homozygous embryos produced by homozygous parents is as low as 4-7%. (Zhang et al., 2003) About 80% of homozygous animals derived from heterozygous parents eclose, although subsequent generations show further decreased viability, probably due to decreased levels of maternal JIL-1 product. Only 8% of embryos derived from JIL-1^z2/JIL-1^EP3657 females mated to wild-type males hatch, of those larvae that hatch, 58% survive to pupation, and of those that survive to pupation 95% eclose. 94% of embryos derived from wild-type females mated to JIL-1^z2/JIL-1^EP3657 males hatch, and of those larvae that hatch, 95% survive to pupation. 87% of embryos derived from JIL-1^z2/+ females mated to JIL-1^z2/JIL-1^EP3657 males hatch, suggesting that some embryos with the JIL-1^z2/JIL-1^z2 or JIL-1^z2/JIL-1^EP3657 genotype can survive embryogenesis if provided with normal maternal JIL-1 product. No JIL-1^z2/JIL-1^z2 progeny survive to eclosion and the number of surviving JIL-1^z2/JIL-1^EP3657 adults is about half that of comparable sibling classes. Homozygous ovaries are moderately decreased in size compared to wild-type ovaries. In 88% of homozygous males, the A6 sternite is covered by one or more large bristles, indicating a transformation of A6 to the more anterior A5 segment. 93% of JIL-1^z2/JIL-1^EP3657 males have ventral sternite bristles on the A6 sternite, indicating a transformation of A6 to the more anterior A5 segment. (Zhang et al., 2003) Mutant larvae expressing JIL-1^EP3657 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects. (Kraut et al., 2001) Homozygous larvae (derived from heterozygous females) have an eclosion rate of 81%. The number of males eclosing is 73% that of females. Eclosed adults are fertile and able to produce offspring. The hatch rate of embryos derived from homozygous parents is only 4%. The number of males eclosing is 48% that of females. Embryos derived from homozygous parents show a range of phenotypes from embryos appearing wild type with regularly spaced nuclei to embryos where chromatin structure is completely disintegrated. In intermediate phenotypes, nuclei in various stages of fragmentation are discernible. Centrosomes are often seen to be separated from the nuclear remnants and in other cases aberrant and misaligned tubulin spindles are seen. The polytene chromosome banding pattern is relatively normal in homozygous third instar larvae. Perturbation of the male X chromosome is relatively more severe than that of the autosomes which are only subtly affected. (Wang et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3657 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 0 )

Linkouts
Comments
	Location 3L:11066257-11066258 confirmed by FlyBase alignment of dbGSS accession AQ073095 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3) 3657 (Kraut et al., 2001, Kraut et al., 2001) P{EP}EP3657 (FlyBase, 1992-) P{EP}JIL-1^EP3657 (FlyBase, 2005, FlyBase, 1992-, )
Secondary FlyBase IDs

References ( 13 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Zhang et al., 2003, Genetics 165(3): 1341--1354 Genetic and phenotypic analysis of alleles of the Drosophila chromosomal JIL-1 kinase reveals a functional requirement at multiple developmental stages. [FBrf0167636] Zhang et al., 2003, J. Biol. Chem. 278(13): 11696--11704 A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1. [FBrf0159022] Kraut et al., 2001, Curr. Biol. 11(6): 417--430 A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135708] Wang et al., 2001, Cell 105(4): 433--443 The JIL-1 tandem kinase mediates histone H3 phosphorylation and is required for maintenance of chromatin structure in Drosophila. [FBrf0136815] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Supplementary material	Kraut et al., 2001, Curr. Biol. 11(6): A gain-of-function screen for genes controlling motor axon guidance and synaptogenesis in Drosophila. [FBrf0135968]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}JIL-1EP3657 Insertion

Dmel\P{EP}JIL-1^EP3657 Insertion