Dmel\P{EP}twf^EP3701 Insertion

General Information
Symbol	Dmel\P{EP}twf^EP3701	Species	D. melanogaster
Name		FlyBase ID	FBti0011782
Feature type	transposable_element_insertion_site
Description
Inserted element	P{EP}	Expression data
Affected gene(s)	twf	Viability / fertility
Causes allele(s)	twf^EP3701	Stock availability	1 publicly available
LINE ID	EP(3)3701
Genomic Location
Chromosomal location	3R ( 88A9 )	Sequence location	3R:9,948,482..9,948,482 [-]
Map ( GBrowse )
Member of Large Scale Dataset(s)
Dataset	TI_set_P{EP}.EP A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity. Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank. (Rorth, 1996, Bellen et al., 2004)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
Chromosome (arm)
Sequence Location	3R:9,948,482..9,948,482 [-] (BDGP Project Members, 2000-)
Orientation
Cytological location (computed by FlyBase)	88A9 ( inferred by FlyBase from sequence location )
Cytological location (reported)	88A10-88A10 (reported as inferred from sequence location) (BDGP Project Members, 2000-)
Comments concerning location
Sequence Data
Flanking sequence	AQ073130 (FlyBase, 1992-,
Inserted Element
Construct	P{EP}
Location-dependent role	mobile activating element (UASG) (Rorth, 1996)
Size	7.987Kb
Associated alleles	Scer\UAS^14x.hs TrlBR^cRa w^+mC
Molecular map
Affected Gene(s)
Insertion may affect gene	twf (BDGP Project Members, 2000-, Stephan et al., 2011, Parrish et al., 2009, Wang et al., 2010)
Alleles and Phenotypes
Causes alleles	twf^EP3701 (Gene Disruption Project members, 2001-, Wahlstrom et al., 2001, Stephan et al., 2011, Parrish et al., 2009, Wang et al., 2010)

Lethality References viable (Wahlstrom et al., 2001)
Sterility References
Phenotype Manifest In
	adult mushroom body (Wang et al., 2010) border follicle cell \| heat sensitive (Wang et al., 2010) chaeta (Wahlstrom et al., 2001) interommatidial bristle (Wahlstrom et al., 2001) macrochaeta (Wahlstrom et al., 2001) microchaeta (Wahlstrom et al., 2001) mushroom body alpha-lobe (Wang et al., 2010) mushroom body beta-lobe (Wang et al., 2010) ommatidium (Wahlstrom et al., 2001) trichome (Wahlstrom et al., 2001)
Detailed Description
Statement Reference Expression of twf[Scer\UAS.cWa] under the control of Scer\GAL4[ey-OK107] rescues the crossing over and, in severe cases, fusion of the β lobe of the adult mushroom body in twf[EP3701] mutant animals. (Wang et al., 2010) β lobes of the mushroom body cross the midline in approximately 50% of twf[EP3701] and 30% twf[EP3701]/Df(3R)su(Hw)7 mutant animal brains. In severe cases, the two β lobes are completely fused. β loves are bifurcated and thickened. 10% of twf[EP3701] mutants display unilateral or bilateral loss of the adult mushroom body α lobe. At 29[o]C, twf[EP3701] and twf[EP3701]/twf[110] mutant animals display a moderate but significant delay in border cell migration. (Wang et al., 2010) Nearly all twf^EP3701 tsr^k05633 double heterozygotes have at least one macrochaeta with defects at the bristle tip. The posterior scutellar and anterior dorsocentral bristles are the most frequently affected. 66% of bristles are split, branched or have a rough surface in these animals. tsr^k05633/+ enhances the eye defects seen in twf^EP3701 homozygotes. (Wahlstrom et al., 2001) Homozygous flies are slightly smaller and less active than wild-type flies. They show reduced or completely lost flight ability. Hatching frequency of twf^EP3701 animals is slightly reduced, the larval period is significantly longer than normal, but the duration of the pupal period is wild type. No defects in border cell migration are seen in twf^EP3701 egg chambers. twf^EP3701 mutants have a rough eye phenotype. The interommatidial bristles are often tufted and the ommatidia are sometimes pitted and occasionally fused. All bristles show defects in morphology, with macrochaetae being more severely affected than microchaetae. The macrochaetae are shorter than normal and have a rough surface, with the ridges and grooves on the surface of the macrochaetae being highly irregular (in contrast to the completely straight ridges and grooves seen in wild-type bristles). The bristles often have a thicker portion somewhere along the shaft, and in this region the ridges are oriented perpendicular to the long axis. The tip of the bristle is not as thin as normal and has a smooth surface with only short, randomly oriented ridges. The macrochaetae, but not microchaetae, are often split, bent or branched. The hairs are also slightly shorter than normal. Mutant 48 hours old macrochaetae have a large number of small F-actin containing spots or tiny actin bundles. The spots are localised at the bristle surface between the main bundles and the tiny bundles appear to be oriented perpendicular to the long axis of the bristle. (Wahlstrom et al., 2001)
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Reporter construct used in assay
External Images
FlyView (LinkOut)
Data on Genetic Line
Line ID	EP(3)3701 (Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome

Related Aberration or Balancer
Aberration
Balancer
Stocks ( 1 )
Bloomington	17157 w¹¹¹⁸; P{EP}twf^EP3701/TM6B, Tb¹
Linkouts
Comments
	Location 3R:9948482-9948483 confirmed by FlyBase alignment of dbGSS accession AQ073130 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed. (FlyBase, 2005) insertion of mobile activating element (BDGP Project Members, 1994-1999)
Synonyms & Secondary IDs
Reported As
Symbol Synonym	EP(3)3701 (Wahlstrom et al., 2001, FlyBase, 1992-, Wang et al., 2010) P{EP}CG3172^EP3701 (FlyBase, 1992-) P{EP}EP3701 (FlyBase, 1992-) P{EP}twf^EP3701 (FlyBase, 2005, FlyBase, 1992-, ) unnamed (Parrish et al., 2009)
Secondary FlyBase IDs

References ( 12 )
Research paper	Stephan et al., 2011, Mol. Biol. Cell 22(21): 4079--4092 Membrane-targeted WAVE mediates photoreceptor axon targeting in the absence of the WAVE complex in Drosophila. [FBrf0216499] Wang et al., 2010, J. Cell Sci. 123(9): 1546--1556 Drosophila twinfilin is required for cell migration and synaptic endocytosis. [FBrf0210650] Parrish et al., 2009, Neuron 63(6): 788--802 The microRNA bantam functions in epithelial cells to regulate scaling growth of dendrite arbors in drosophila sensory neurons. [FBrf0208858] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Wahlstrom et al., 2001, J. Cell Biol. 155(5): 787--795 Twinfilin is required for actin-dependent developmental processes in Drosophila. [FBrf0141598] Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422 A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase	Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078] BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis	FlyBase Curators, 2013, Members of TE insertion collections. Members of TE insertion collections. [FBrf0220668] FlyBase, 2005, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0184339] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]

Dmel\P{EP}twfEP3701 Insertion

Dmel\P{EP}twf^EP3701 Insertion