A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Dmel\P{EP}aosEP3733 Insertion

General Information
Symbol Dmel\P{EP}aosEP3733 Species D. melanogaster
Name FlyBase ID FBti0011811
Feature type transposable_element_insertion_site
Description
Inserted element P{EP} Expression data
Affected gene(s) aos, CG33158 Viability / fertility
Causes allele(s) aosEP3733, CG33158EP3733 Stock availability none publicly available
LINE ID EP(3)3733
Genomic Location
Chromosomal location 3L ( 73A2 ) Sequence location 3L:16,476,568..16,476,568 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBti0143279 FBti0047449 FBti0125736 FBti0049703 FBti0067386 FBti0053971 FBti0011811 FBti0029617 FBti0057485 FBti0005546 FBti0145261 FBti0029723 FBti0110843 FBti0039727 FBti0103331 FBti0104805 FBti0009535 FBti0068512 FBti0072474 FBti0111247 FBti0054717 FBti0029130 FBti0016562 FBti0053266 FBti0033275 FBti0050640
Member of Large Scale Dataset(s)
Dataset

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EP}. The P{EP} construct construct carries a w[+mC] mini-white visible marker, Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator, and bacterial sequences that allow plasmid rescue. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were mapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
(Rorth, 1996, Bellen et al., 2004)
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:16,476,568..16,476,568 [+]
(BDGP Project Members, 2000-)
Orientation
Cytological location
(computed by FlyBase)
73A2 ( inferred by FlyBase from sequence location )
Cytological location
(reported)
73A1-73A2 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
73A3-73A3 (reported as inferred from sequence location)
(BDGP Project Members, 2000-)
Comments concerning
location
hide Sequence Data
Flanking sequence
AQ073155
(FlyBase, 1992-,  
hide Inserted Element
Construct P{EP}
Location-dependent
role
mobile activating element (UASG)
(Rorth, 1996)
Size 7.987Kb
Associated alleles
Scer\UAS14x.hs
TrlBRcRa
w+mC
Molecular map
hide Affected Gene(s)
Insertion may
affect gene
aos
(BDGP Project Members, 2000-)
CG33158
(FlyBase, 1996-)
hide Alleles and Phenotypes
Causes alleles
aosEP3733
(Gene Disruption Project members, 2001-, FlyBase, 1996-)
CG33158EP3733
(FlyBase, 1996-)
Lethality
References
Sterility
References
hide Phenotype Manifest In
hide Detailed Description
Statement
Reference
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects
expression of
Reporter construct
used in assay
hide External Images
FlyView (LinkOut)
hide Data on Genetic Line
Line ID
EP(3)3733
(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
hide Progenitor(s) within the Genome
hide Related Aberration or Balancer
Aberration
Balancer
hide Stocks ( 0 )
hide Linkouts
hide Comments
Location 3L:16448727-16448728 confirmed by FlyBase alignment of dbGSS accession AQ073155 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.
(FlyBase, 2005)
insertion of mobile activating element
(BDGP Project Members, 1994-1999)
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
EP(3)3733
(FlyBase, 1992-)
P{EP}aosEP3733
 
P{EP}argosEP3733
(FlyBase, 1992-, FlyBase, 2005)
P{EP}EP3733
(FlyBase, 1992-)
Secondary FlyBase IDs
hide References ( 9 )
Research paper
Bellen et al., 2004, Genetics 167(2): 761--781
The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132]
Rorth, 1996, Proc. Natl. Acad. Sci. U.S.A. 93(22): 12418--12422
A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. [FBrf0090768]
Personal communication to FlyBase
Gene Disruption Project members, 2001-, (Computer file)
(Computer file) [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file)
Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
BDGP Project Members, 1994-1999, BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file)
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project. (Computer file) [FBrf0067338]
FlyBase analysis
FlyBase Curators, 2013, Members of TE insertion collections.
Members of TE insertion collections. [FBrf0220668]
FlyBase, 2005, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0184339]
FlyBase, 1996-, FlyBase inference based on genome sequence analysis.
FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]